[1]侯 沪,李爱敏,唐曙明.临床嗜水气单胞菌耐药性及Aer气胞溶素基因研究[J].现代检验医学杂志,2015,30(02):49-51.[doi:10.3969/j.issn.1671-7414.2015.02.015]
　HOU Hu,LI Ai-min,TANG Shu-ming.Research on Clinical Resistance and Aerolysin Genes of Aeromonas Hydrophila[J].Journal of Modern Laboratory Medicine,2015,30(02):49-51.[doi:10.3969/j.issn.1671-7414.2015.02.015]
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临床嗜水气单胞菌耐药性及Aer气胞溶素基因研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
49-51
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Research on Clinical Resistance and Aerolysin Genes of Aeromonas Hydrophila
作者:
侯 沪; 李爱敏; 唐曙明
深圳市人民医院龙华分院,广东深圳 518000
Author(s):
HOU Hu; LI Ai-min; TANG Shu-ming
People's Hospital of Shenzhen Longhua Branch,Guangdong Shenzhen 518000,China
关键词:
嗜水气单胞菌;  耐药性;  气胞溶素基因Aer
分类号:
R378.99; Q781
DOI:
10.3969/j.issn.1671-7414.2015.02.015
文献标志码:
A
摘要:
目的 研究嗜水气单胞菌临床感染现状及其主要致病因子Aer气胞溶素基因的存在形式。方法 收集深圳市人民医院龙华分院2012年～2013年间临床分离到的嗜水气单胞菌,VITEK 2 compact对其进行鉴定与抗生素药物敏感性试验。数据录入WHONET5.6软件进行临床分布与耐药率分析。提取染色体与质粒基因组,采用聚合酶链反应(PCR)法检测毒力基因Aer气胞溶素的存在形式。结果 临床共分离到48株嗜水气单胞菌,分布于临床标本痰液16株、血液11株、分泌物10株、尿液7株与粪便4株。病区分布分散化,无集中趋势。耐药率显示对氨苄西林、氨苄西林/舒巴坦与头孢唑啉耐药率达80%以上,而对阿米卡星、头孢吡肟、左旋氧氟沙星、哌拉西林/他唑巴坦与亚胺培南处于10%以下的低水平。43.7%菌株携带Aer基因,位于染色体基因组。结论 嗜水气单胞菌临床感染散发,多数染色体基因组携带毒力因子Aer气胞溶素基因。对青霉素类,一代头孢类耐药严重,对广谱类药物保持敏感性。嗜水气单胞菌作为地区性重要条件致病菌,防范其耐药菌株扩散具有一定意义。
Abstract:
Objective To study Aeromonas hydrophila infection and the clinical status of the major causative factor Aerolysin gene.Methods Clinical isolates of Aeromonas hydrophila was collected from 2012 to 2013 year in People's Hospital of Shenzhen Longhua Branch.Its identification and antibiotic susceptibility testing was analyzed by VITEK2 compact.Clinical resistance rates and distribution was analyzed by WHONET5.6 software.Polymerase chain reaction(PCR)was used to detect the Aerolysin gene form in chromosomes and plasmids extracted genome.Results The clinical total of 48 isolated Aeromonas hydrophila,distributed in 16 clinical samples of sputum,blood 11,10 secretions,urine 7 and stool 4.Distribution in ward decentralized,the central tendency was not detected.Drug resistance rates to ampicillin,ampicillin/sulbactam and cefazolin reached more than80%,while amikacin,cefepime,levofloxacin,piperacillin/tazobactam and imipenem was low 10% or less.43.7% strains carried the Aerolysin gene.39.5% of Aerolysin gene was found on chromosome genome.Conclusion Aeromonas hydrophila clinical infection existed in dispersed form,most of which carried Aerolysin gene in chromosome genome.Aeromonas hydrophila had serious resistance to penicillins and first generation cephalosporin,but to broad-spectrum drugs maintaining high sensitivity.Precaution of Aeromonas hydrophila,as an important condition pathogenic bacteria,is some significant for preventing it's proliferation of drug-resistant strains.

参考文献/References:

[1] 葛樯樯,白永凤,陈 晓,等.腹泻患者粪便来源的气单胞菌分布与耐药性[J].中国老年学杂志,2013,33(21):5228-5230. Ge QQ,Bai YF,Chen X,et al.Aeromonas distribution and drug resistance of patients with diarrhea faecal sources[J].Chinese Journal of Gerontology,2013,33(21):5228-5230.
[2] 费 怡,孙 乔,傅益飞,等.上海市浦东新区5岁以下儿童腹泻病原谱监测分析[J].上海预防医学,2013,25(11):602-605. Fei Y,Sun Q,Fu YF,et al.Surveillance and analysis on diarrheal disease pathogen spectrum among children under 5 years old in Pudong New Area ofShanghai City[J].Shanghai Journal of Preventive Medicine,2013,25(11):602-605.
[3] Wang G,Clark CG,Liu C,et al.Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR[J].Journal of Clinical Microbiology,2003,41(3):1048-1054.
[4] 任超杰,扈会整,汪建军,等.医院感染嗜水气单胞菌的临床治疗分析[J].现代检验医学杂志,2014,29(4):128-129,131. Ren CJ,Hu HZ,Wang JJ,et al.Clinical treatment analysis of Aeromonas hydrophila for hospital infection[J].Journal of Modern Laboratory Medicine,2014,29(4):128-129,131.
[5] Nawaz M,Khan SA,Khan AA,et al.Detection and characterization of virulence genes and integrons in Aeromonas veronii isolated from catfish[J].Food Microbiology,2010,27(3):327-331.
[6] 陈素明,鲍春梅,崔恩博,等.北京地区气单胞的流行状况及耐药性分析[J].中国抗生素杂志,2011,36(5):374-376,S2. Chen SM,Bao CM,Cui EB,et al.Prevalence and antimicrobial resistance analysis of Aeromonas in Beijing[J].Chinese Journal of Antibiotics,2011,36(5):374-376,S2.
[7] Yu HB,Zhang YL,Lan YL,et al.Identification and characterization of putative virulence of genes and gene clusters in Aeromonas hydrophila PPD134/91[J].Appl Environ Microbiol,2005,71(8):4469-4477.
[8] 付乔芳,邱军强,胡 鲲,等.嗜水气单胞菌毒力基因在传代过程中的稳定性研究[J].生物技术通报,2011(9):130-135. Fu QF,Qiu JQ,Hu K,et al.Stability of virulence genes of Aeromonas hydrophila strains during subculture[J].Biotechnology Bulletin,2011(9):130-135.
[9] Han JE,Kim JH,Choresca BCH,et al.A small lncQ-type plasmid carryingthe quinolone resistance(qnrS2)gene from Aeromonas hydrophila[J].Lett Appl Microbiol,2012,54(4):374-376.
[10] 曲 芬,崔恩博,鲍春梅,等.气单胞菌的耐药基因研究[J].中华医院感染学杂志,2005,15(3):249-252. Qu F,Cui EB,Bao CM,et al.Resistant of genes of Aeromonas[J].Chin J Nosocomiol,2005,15(3):249-252.

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备注/Memo

备注/Memo:
作者简介:侯 沪(1979-),女,本科,主管技师,研究方向:临床微生物检验,E-mail:cd wan@163.com。
更新日期/Last Update: 2015-03-20