[1]谢小娟,朱 娜,潘晶晶,等.miRNA-148a在膀胱癌组织中的表达及生物信息学分析[J].现代检验医学杂志,2015,30(04):6-9.[doi:10.3969/j.issn.1671-7414.2015.04.002]
　XIE Xiao-juan,ZHU Na,PAN Jing-jing,et al.Expression of miRNA-148a in Bladder Carcinoma Tissues and Its Bioinformatics Analysis[J].Journal of Modern Laboratory Medicine,2015,30(04):6-9.[doi:10.3969/j.issn.1671-7414.2015.04.002]
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miRNA-148a在膀胱癌组织中的表达及生物信息学分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
6-9
栏目:
论著
出版日期:
2015-08-10

文章信息/Info

Title:
Expression of miRNA-148a in Bladder Carcinoma Tissues and Its Bioinformatics Analysis
文章编号:
1671-7414(2015)04-006-05
作者:
谢小娟1; 2; 朱 娜3; 潘晶晶2; 魏力强1; 陈葳2
1.陕西省临床检验中心,西安 710068;
2.西安交通大学医学院第一附属医院检验科,西安 710061;
3.陕西省人民医院科研处,西安 710068
Author(s):
XIE Xiao-juan1; 2; ZHU Na3; PAN Jing-jing2; WEI Li-qiang1; CHEN Wei2
1.Shaanxi Center for Clinical Laboratory,Xi'an 710068,China;
2.Department of Clinical Laboratory,the First Affiliated Hospital,School of Medicine,Xi'an Jiaotong University,Xi'an 710061,China;
3.Scientific Research Division,Shaanxi Provincial
关键词:
膀胱癌;  miRNA-148a;  生物信息学
分类号:
R737.14; R730.43
DOI:
10.3969/j.issn.1671-7414.2015.04.002
文献标志码:
A
摘要:
目的 探索miRNA-148a在膀胱癌发生发展中的作用。方法 收集35例膀胱癌组织和16例非癌组织,采用荧光定量PCR方法检测miRNA-148a的相对表达量,并分析其与临床特征的相关性; 采用生物信息学分析,预测miRNA-148a的靶基因和转录因子,构建TF-miRNA-148a-靶基因调控网络图,并对其靶基因进行GO富集和KEGG Pathway分析。结果 miRNA-148a在膀胱癌中的相对表达量(0.0008±0.0002)明显低于非癌组织(0.0021±0.0005)(t=2.46,P＜0.05),其相对表达量在不同性别、年龄、病理分级、临床分期、淋巴结转移组的差异无统计学意义(P＞0.05)。3个软件都预测到的miRNA-148a靶基因268个; 预测到miRNA-148a的转录因子60个,结合评分＞80的结合位点657个; 对268个靶基因进行GO富集分析,发现其靶基因主要涉及神经细胞分化、发育、增殖、mRNA合成,蛋白连接等众多的生物学过程(P＜0.001,相对于背景具有统计意义); KEGG Pathway分析发现268个靶基因参与p53,恶性肿瘤、前列腺癌、PI3K-AKT等信号通路(P＜0.05,相对于背景具有统计意义); 根据构建的TF-miRNA-148a-靶基因调控网络图,挖掘出可能调控miRNA-148a的转录因子有SP1,ESR1,AP1,MYC,BRCA1等; 可能受miRNA-148a调控的基因有IGF1,P27kip1,NCOA1,PTEN,SERPINE1等。结论 miRNA-148a在膀胱癌中表达下调,可能参与膀胱癌的发生发展,生物信息学分析为后续研究提供一定的思路。
Abstract:
Objective To explore the role of miRNA-148a inbladder tumorous development and progression.Methods Expression of miRNA-148a was assessed in 35 bladder carcinoma tissues and 16 non-carcinoma tissues by fluorescence quantitative real time PCR,and correlationwith clinical features was evaluated.Target genes and transcription factors ofmiRNA-148a were predicted using bioinformatic analysis,then TF-miRNA-148a-target genes network diagram was built and the target genes was analyzed of geneontology enrichment and KEGG pathway.Results Expressionof miRNA-148a was lower in bladder carcinoma tissues than in non-carcinoma tissues(0.000 8±0.000 2 vs 0.002 1±0.000 5)(t=2.46,P＜0.05),but itsexpression was no statistical significance in different groups of gender,age,pathological classification,clinical stage,lymph node metastasis(P＞0.05).268 target genes of miRNA-148a were predicted by three softwares at the sametime,60 transcription factors were predicted and the binding sites with combination scroes above 80 was 657.The target genes of miRNA-148a was enriched in many biological processes,such as neuron differentiation,generation of neurons,neuron projection development,cytoplasmic mRNA processing body,cytoplasm(P＜0.001).They also participated in p53 signaling pathway,proteoglycans in cancer-homo sapiens,pathways in cancer,prostate cancer,protein processing inendoplasmic reticulum,focal adhesion and so on(P＜0.05).According to TF-miRNA-148a-target genes network diagram,miRNA-148a was regulated by SP1,ESR1,AP1,MYC and BRCA1,genes of IGF1,P27kip1,NCOA1,PTEN,SERPINE1 mightbe regulated by miRNA-148a.Conclusion miRNA-148a which was significantly down-regulation in bladder carcinoma tissues may be participate in bladder tumorous development and progression,bioinformatics analysis provides some ideas for further research.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81372151)。
作者简介:谢小娟(1981-),女,硕士,主要从事分子生物学研究。
通讯作者:陈 葳(1962-),女,博士生导师,E-mail:chenwei808@mail.xjtu.edu.cn。
更新日期/Last Update: 2015-08-10