[1]许有才,赵世元.IL-3在肺炎支原体感染小鼠体内的表达及相关机制[J].现代检验医学杂志,2015,30(04):102-104.[doi:10.3969/j.issn.1671-7414.2015.04.029]
　XU You-cai,ZHAO Shi-yuan.Expressions of Interleukin-3 and Their Mechanisms in Mice Infected by Mycoplasma Pneumoniae[J].Journal of Modern Laboratory Medicine,2015,30(04):102-104.[doi:10.3969/j.issn.1671-7414.2015.04.029]
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IL-3在肺炎支原体感染小鼠体内的表达及相关机制()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
102-104
栏目:
研究简报·实验技术
出版日期:
2015-08-10

文章信息/Info

Title:
Expressions of Interleukin-3 and Their Mechanisms in Mice Infected by Mycoplasma Pneumoniae
文章编号:
1671-7414(2015)04-102-03
作者:
许有才1; 赵世元2
1.广西大新县人民医院检验科,广西大新 532300;
2.广西医科大学附属民族医院检验科,南宁 530001
Author(s):
XU You-cai1; ZHAO Shi-yuan2
1.Department of Clinical Laboratory,Daxin County People's Hospital in Guangxi,Guangxi Daxin 532300,China;
2.Department of Clinical Laboratory,the Affiliated National Hospital of Guangxi Medical University National Hospital,Nanning530001,China
关键词:
肺炎支原体;  白细胞介素IL-3;  克拉霉素
分类号:
R375.2; R-332
DOI:
10.3969/j.issn.1671-7414.2015.04.029
文献标志码:
A
摘要:
目的 研究IL-3在BALB/c小鼠肺炎支原体(MP)感染模型肺泡灌洗液和血清中的表达,阐明其与MP感染的关系。方法 36只BALB/c裸鼠,随机分为3组,分别为对照组、MP感染组和克拉霉素组,每组12只。用PPLO肉汤培养基MP,将成功培养的MP鼻腔滴入裸鼠建立MP感染模型。治疗组用克拉霉素连续治疗8天,收集并分离血清,取其肺组织,收集肺泡灌洗液,采用ELISA法测定小鼠血清和肺泡灌洗液中IL-3水平,肺部组织用中性甲醛固定HE染色,观察MP感染小鼠肺的病变程度。结果 成功建立MP感染小鼠模型,病理组织学观察结果显示,MP感染BALB/c小鼠后炎症明显,克拉霉素治疗BALB/c小鼠炎症逐渐减轻。模型组血清和肺泡灌洗液中IL-3分别为96.4±8.3 ng/L和60.9±6.5 ng/L,与对照组比较(203.1±14.5 ng/L,96.3±12.6 ng/L),MP感染组小鼠血清和肺泡灌洗液中IL-3水平明显降低(t血清=19.50,t肺泡= 17.20,P值均＜0.01),用克拉霉素治疗后,小鼠血清和肺泡灌洗液中IL-3明显升高,分别为175.6±12.5 ng/L和85.6±10.2 ng/L,差异有统计学意义,t血清=17.41,t肺泡= 14.62,P值均＜0.01)。结论 肺炎支原体感染使小鼠IL-3水平降低,克拉霉素治疗有一定疗效,IL-3表达可能与MP肺炎严重程度有关。
Abstract:
Objective To investigate the expressions of interluekin-3 in BALB/c mice infected by mycoplasma pneumonia(MP)and to clarify the relationship between IL-3 and MP infection.Methods Total 36 BALB/c mice were randomly divided into control group,MP infection group and clarithromycin treatment group.MP infection models were established with the method of dripping cultured MP into nasal cavity of mice.ELISA method was used to assay the levels of IL-3 in the the serum of the mice and bronchoalveolar lavage fluid in various groups.The lung tissue was fixed in formalin for sections,HE staining was performed for the observation of histological changes.Results The MP-infected mouse models were established successfully and MP strains were dripped into the respiratory system of mice.As showed in histopathological observasion results,significantly increased after MPinfection and then gradually decreased after clarithromycin treatment.The ELISA results showed that the level of serum and bronchoalveolar lavage fluid of IL-3 of the mice in MP infection group were 96.4±8.3 ng/L and 60.9 ±6.5 ng/L.Compared with the control group(203.1±14.5 ng/L,96.3±12.6ng/L),serum andbronchoalveolar lavage fluid of mice infected with MP in IL-3 levels were significantly lower(tserum= 19.50,tbalf=17.20,P＜0.01),and after treatment with clarithromycin,serum and bronchoalveolar lavage fluid of IL-3significantly increased,respectively(175.6±12.5 ng/L and 85.6±10.2 ng/L,tserum=17.41,tbalf=14.62,P＜0.01),the difference was statistically significant.Conclusion IL-3 levels were decreased in mycoplasma pneumonia mice.Clarithromycin treatment had effect on mycoplasma pneumonia infecttion.The expression level IL-3 may be related to the severity degree of MP infection.

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备注/Memo

备注/Memo:
基金项目:广西医疗卫生科研课题(Z2014029)。
作者简介:许有才(1968-),男,主管检验师,主要从事医学免疫研究及检验工作,Tel:13768810313,E-mail:dxwsj2008@163.com。
通讯作者:赵世元(1965-),男,主任技师,主要从事药物开发研究,Tel:13978647518,E-mail:zhaoshiyuan 1105@163.com。
更新日期/Last Update: 2015-08-10