[1]段宝生.耐甲氧西林金黄色葡萄球菌的SPA基因分型研究与耐药性分析[J].现代检验医学杂志,2016,31(02):80-82.[doi:10.3969/j.issn.1671-7414.2016.02.024]
　DUAN Bao-sheng.Resistance Drug Analysis and the Detection of PVL Gene of Methicillin-Resistant Staphylococcus Aureus[J].Journal of Modern Laboratory Medicine,2016,31(02):80-82.[doi:10.3969/j.issn.1671-7414.2016.02.024]
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耐甲氧西林金黄色葡萄球菌的SPA基因分型研究与耐药性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
80-82
栏目:
论著
出版日期:
2016-06-01

文章信息/Info

Title:
Resistance Drug Analysis and the Detection of PVL Gene of Methicillin-Resistant Staphylococcus Aureus
作者:
段宝生
内蒙古医科大学鄂尔多斯临床医学院,内蒙古鄂尔多斯 017000
Author(s):
DUAN Bao-sheng
Medical College of Ordos, Inner Mongolia Medical University,Inner Mongolia Ordos,017000,China
关键词:
耐甲氧西林金黄色葡萄球菌;  杀白细胞毒素;  聚合酶链反应;  琼脂稀释法
分类号:
R378.11; Q786
DOI:
10.3969/j.issn.1671-7414.2016.02.024
文献标志码:
A
摘要:
目的 探讨鄂尔多斯地区耐甲氧西林金黄色葡萄球菌(MRSA)分离株杀白细胞毒素(PVL)基因分布特点及药物敏感性。方法 收集2012年6月～2014年12月临床分离的SCCmecⅡ型和Ⅲ型MRSA菌株66株,应用聚合酶链反应对菌株进行PVL基因型检测,用琼脂稀释法测定PVL阳性菌株对常用抗菌药物的敏感性。结果 66例MRSA有6例PVL阳性,阳性率9.09%,其中Ⅱ型4株,Ⅲ型2株; 6株PVL阳性菌均对万古霉素、替考拉宁、利奈唑胺和对多种非β内酞胺类抗菌药敏感,对β内酞胺类、大环内酯类和克林霉素高度耐药。结论 在鄂尔多斯地区MRSA菌株中PVL阳性菌株较低,4株为CA-MRSA,2株为HA-MRSA,若以PVL阳性作为区分MRSA类型的标志有待进一步研究。
Abstract:
Objective To investigate the distribution of Panton-Valentine leukocidin gene Methicillin-resistant Staphylococcus aureus(MRSA)from Mongolian in Ordos,and the antimicrobial susceptibility of clinical isolates of MRSA strains.Methods A total of 66 MRSA isolates were collected from July 2012 to December 2014 of SCCmec Ⅱand SCCmec Ⅲ.The polymerase chain reaction(PCR)were used to determine PVL gene.The minimal inhibitory concentrations(MICs)of antibioties for all isolates were determined by agar dilution method.Results Among 66 MRSA strains,the PVL genes were found in 9.09%,4 were identified as SCCmec Ⅱ and 2 asSCCmec Ⅲ.All PVL positive strains were susceptible to vancomycin,teicoplanin,linezolid and non-β-lactam antibiotics,all were resistant to β-lactam antibiotics,macrolides and clindamycin.Conclusion The PVLgene positive rate of MRSA isolates was low in Ordos.Among 6 PVL gene positiveisolates of MRSA,four belonged to CA-MRSA,two belonged to HA-MRSA.However,a considerable sign of MRSA strains might be undecided by PVL gene.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:内蒙古自然科学基金(2013MS1116)。 作者简介:段宝生(1969-),男,汉族,硕士,主任检验师,主要从事微生物耐药机制研究,Tel:15049456590。
更新日期/Last Update: 2016-02-20