[1]赵 佳a,左 林b,姚创利a,等.锰超氧化物歧化酶基因变异 与血脂和同型半胱氨酸水平的关系研究[J].现代检验医学杂志,2016,31(03):12-15.[doi:10.3969/j.issn.1671-7414.2016.03.004]
　ZHAO Jiaa,ZUO Linb,YAO Chuang-lia,et al.Study on the Relationship between Manganese Superoxide Dismutase Gene Variants and the Levels of Blood Lipid and Homocysteine[J].Journal of Modern Laboratory Medicine,2016,31(03):12-15.[doi:10.3969/j.issn.1671-7414.2016.03.004]
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锰超氧化物歧化酶基因变异 与血脂和同型半胱氨酸水平的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
12-15
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Study on the Relationship between Manganese Superoxide Dismutase Gene Variants and the Levels of Blood Lipid and Homocysteine
文章编号:
1671-7414(2016)03-012-04
作者:
赵 佳1a; 左 林1b; 姚创利1a; 黎 阳1a; 姜小建1a; 杨军乐2
1.西安市中心医院 a.检验科;
b.放射科,西安 710003;
2.第四军医大学唐都医院放射科,西安 710038
Author(s):
ZHAO Jia1a; ZUO Lin1b; YAO Chuang-li1a; LI Yang1a; JIANG Xiao-jian1a;  YANG Jun-le2
1a.Department of Chinical Laboratory;
1b.Depatment ofRadiology, Xi'an Central Hospital,Xi'an 710003,China;
2.Department of Radiology, Tangdu Hospital of the Fourth Military Medical University,Xi'an 710038,China
关键词:
锰超氧化物歧化酶;  基因多态性;  血脂;  同型半胱氨酸;  冠心病
分类号:
Q754; R446.12
DOI:
10.3969/j.issn.1671-7414.2016.03.004
文献标志码:
A
摘要:
目的 探讨锰超氧化物歧化酶(Mn-SOD)9 Ala/Val基因多态性与血脂和同型半胱氨酸(HCY)水平的关系。方法 应用测序法检测137例冠心病患者和85例对照组的Mn-SOD 9 Ala/Val基因多态性的基因型,应用比色法检测血清总超氧化物歧化酶(T-SOD)和Mn-SOD活性,应用终点法测定血清胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平,应用酶法测定血清HCY水平。结果 冠心病组的Mn-SOD 9 VV基因型和V等位基因频率显著高于对照组。冠心病组的T-SOD和Mn-SOD活性显著低于对照组。Mn-SOD 9 VV基因型的T-SOD和Mn-SOD活性显著低于Mn-SOD 9 AA基因型。Mn-SOD 9 VV基因型的血清TC,TG,LDL-C和HCY水平显著高于Mn-SOD 9 AA基因型,HDL-C水平显著低于Mn-SOD 9 AA基因型。Mn-SOD活性与血清TC,TG,LDL-C和HCY水平呈负相关,与血清HDL-C水平呈正相关。结论冠心病患者机体的抗氧化能力降低。Mn-SOD 9 Ala/Val基因多态性通过影响Mn-SOD活性进而导致血脂代谢紊乱,促进冠心病的发生发展。HCY通过自身氧化和抑制Mn-SOD活性,致使体内氧化物质增多,增加冠心病的发病风险。
Abstract:
Objective To investigate the relationship between manganese superoxide dismutase(Mn-SOD)9 Ala/Val genetic polymorphisms andthe levels of blood lipid and homocysteine(HCY).Methods The genotypes of Mn-SOD 9 Ala/Val genetic polymorphisms were identified by sequencing method,the serum activities of T-SOD and Mn-SOD were detected by colorimetric method,the serum level of HCY was detected by enzymatic method,and the serum levels of cholesterol(TC),triglyceride(TG),high density lipoproteincholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)were detected by end-point method in 137 patients with coronary heart disease(CHD)and85 controls.Results Compared with the control group,theVV genotype and V allele of Mn-SOD 9 Ala/Val genetic polymorphisms in the CHDgroup were higher,while the serum activities of T-SOD and Mn-SOD in the CHD group was significantly lower.The serum activities of T-SOD and Mn-SOD of theMn-SOD 9 VV genotype was significantly lower than the Mn-SOD 9 AA genotype.Compared with the Mn-SOD 9 AA genotype,the serum levels of TC,TG,LDL-C and HCY of the Mn-SOD 9 VV genotype were significantly higher,while the serum levelof HDL-C was significantly lower.The serum activity of Mn-SOD was negativelycorrelated with the serum levels of TC,TG,LDL-C and HCY and positively correlated with the serum level of HDL-C.Conclusion The antioxidative ability in patients with CHD was decreased.Mn-SOD 9 Ala/Val genetic polymorphisms led to lipid metabolism disorders by affecting the Mn-SOD activity,promoting the development of CHD.HCY resulted in increased oxidative substances by self-oxidation and inhibition of the Mn-SOD activity,increasing the risk of CHD.

参考文献/References:

[1] Matsushima S,Kuroda J,Ago T,et al.Increased oxidative stressin the nucleus caused by Nox4 mediates oxidation of HDAC4 and cardiac hypertrophy[J].Circ Res,2013,112(4):651-663.
[2] Altenh(¨overo)fer S,Kleikers PW,Radermacher KA,et al.The NOX toolbox:validating the role of NADPH oxidases in physiology and disease[J].Cell Mol Life Sci,2012,69(14):2327-2343.
[3] Lee R,Margaritis M,Channon KM,et al.Evaluating oxidative stress in human cardiovascular disease:methodological aspects and considerations[J].CurrMed Chem,2012,19(16):2504-2520.
[4] 姚创利,赵 佳,黎 阳,等.锰超氧化物歧化酶9 Ala/Val基因多态性对冠心病的影响[J].现代检验医学杂志,2015,30(2):1-2,6. Yao CL,Zhao J,Li Y,et al.Effects of manganese superoxide dismutase 9 Ala/Val genetic polymorphisms on coronary heart disease[J].Journal of Modern Laboratory Medicine,2015,30(2):1-2,6.
[5] 杨茂平,叶林秀,邱 红,等.锰超氧化物歧化酶9 Ala/Val基因多态性与冠心病的关系[J].武汉大学学报(医学版),2008,29(6):775-779. Yang MP,Ye LX,Qiu H,et al.Association between manganese superoxide dismutase 9 Ala/Val genetic polymorphisms and coronary artery disease[J].Medical Journal of Wuhan University,2008,29(6):775-779.
[6] Fujimoto H,Taguchi J,Imai Y,et al.Manganese superoxide dismutase polymorphism affects the oxidized low-density lipoprotein-induced apoptosis of macrophages and coronary artery disease[J].Eur Heart J,2008,29(10):1267-1274.
[7] Erhardt LR,Leiter LA,Hobbs FD.Lipid management in cardiovascular disease prevention guidelines:strategies and tactics for implementation[J].Atherosclerosis,2008,196(2):532-541.
[8] Deedwania P,Singh V,Davidson MH.Low high-density lipoprotein cholesterol and increased cardiovascular disease risk:an analysis of statin clinical trials[J].Am J Cardiol,2009,104(10 Suppl):3E-9E.
[9] Smith JD.Myeloperoxidase,inflammation,and dysfunctional high-density lipoprotein[J].J Clin Lipidol,2010,4(5):382-388.
[10] Ohashi M,Runge MS,Faraci FM,et al.MnSOD deficiency increasesendothelial dysftinction in ApoE-deficient mice[J].Arterioscler Thromb VaseBiol,2006,26(10):2331-2336.
[11] Hazen S.HDL structure,function,therapeutics,and imaging[J].Arterioscler Thromb Vase Biol,2010,30(2):138.
[12] Thomas MJ,Chen Q,Zabalawi M,et al.Is the oxidation of high-densitylipoprotein lipids different than the oxidation of low-density lipoprotein lipids[J]. Biochemistry,2001,40(6):1719-1724.
[13] 赵 佳,姚创利,左 林,等.冠心病患者血清同型半胱氨酸对血脂和锰超氧化物歧化酶的影响[J].现代检验医学杂志,2015,30(5):44-45,49. Zhao J,Yao CL,Zuo L,et al.Effects of homocysteine on blood lipid and manganese superoxide dismutase in patients with coronary heart disease[J].Journal of Modern Laboratory Medicine,2015,30(5):44-45,49.
[14] Ignashkova TI,Mesitov MV,Rybakov AS,et al.De-position of von willebrand factor in human endothelial cells HUVEC in the endoplasmic reticulum stressinduced by an excess of homocysteine in vitro[J].Patol Fiziol Eksp Ter,2012(3):81-86.
[15] Caylak E,Aytekin M,Halifeoglu I.Antioxidant effe-cts of methionine,alpha-lipoic acid,N-acetylcysteine and homocysteine on lead-induced oxidative stress to erythrocytes in rats[J].Exp Toxicol Pathol,2008,60(4/5):289-294.

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备注/Memo

备注/Memo:
基金项目:陕西省科学技术研究发展计划项目(项目编号:2013K12-02-10)。 作者简介:赵 佳(1984-),女,博士,主管检验师,主要从事临床生物化学检验工作,E-mail:84911zj@163.com。 通讯作者:姜小建(1968-),男,本科,主任检验师,主要从事临床医学检验工作,E-mail:jiangxiaojian1005@126.com。
更新日期/Last Update: 2016-06-25