[1]李瑜珍,曾学辉,莫 莉,等.VITEK2 Compact全自动微生物分析仪对黏液型和非黏液型铜绿假单胞菌药敏检测评价[J].现代检验医学杂志,2016,31(04):121-124.[doi:10.3969/j.issn.1671-7414.2016.04.034]
　LI Yu-zhen,ZENG Xue-hui,Mo-li,et al.Evaluation of Drug Susceptibility Test to Mucoid Pseudomonas Aeruginosa and Non-Mucoid Pseudomonas Aeruginosa with VITEK2 Compact Automatic Microbiology Analyzer[J].Journal of Modern Laboratory Medicine,2016,31(04):121-124.[doi:10.3969/j.issn.1671-7414.2016.04.034]
点击复制

VITEK2 Compact全自动微生物分析仪对黏液型和非黏液型铜绿假单胞菌药敏检测评价()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年04期
页码:
121-124
栏目:
研究简报·实验技术
出版日期:
2016-08-10

文章信息/Info

Title:
Evaluation of Drug Susceptibility Test to Mucoid Pseudomonas Aeruginosa and Non-Mucoid Pseudomonas Aeruginosa with VITEK2 Compact Automatic Microbiology Analyzer
文章编号:
1671-7414(2016)04-121-04
作者:
李瑜珍1; 曾学辉1; 莫 莉2; 蓝志明1; 丁丽娜1
1.深圳市中医院检验科,广东深圳 518034;
2.深圳市福田区中医院检验科,广东深圳 518033
Author(s):
LI Yu-zhen1; ZENG Xue-hui1; Mo-li2; LAN Zhi-ming1; DING Li-na1
1.Department of Laboratory Medicine,Shenzhen TCM Hospital, Guangdong Shenzhen 518034,China;
2.Department of Laboratory Medicine, Shenzhen Futian District TCM Hospital,Guangdong Shenzhen 518033,China
关键词:
铜绿假单胞菌;  药敏试验;  准确性;  VITEK2 Compact
分类号:
R446.5
DOI:
10.3969/j.issn.1671-7414.2016.04.034
文献标志码:
A
摘要:
目的 评价VITEK2 Compact全自动微生物分析仪对黏液型和非黏液型铜绿假单胞菌药敏检测结果的准确性。方法 对某院分离的29株黏液型铜绿假单胞菌和30株非黏液型铜绿假单胞菌同时以VITEK2 Compact分析仪法及琼脂稀释法进行药物敏感性试验,以琼脂稀释法为参考方法进行统计学分析。结果黏液型铜绿假单胞菌VITEK2 Compact分析仪法药敏结果的标准符合率(CA)为84.69%,严重错误率(VME)为3.20%,重大错误率(ME)为2.13%,一般错误率(MIE)为9.96%; 非黏液型铜绿假单胞菌VITEK2 Compact分析仪法药敏结果的标准符合率(CA)为94.33%,严重错误率(VME)为0.67%,重大错误率(ME)为1.67%,一般错误率(MIE)为3.33%。两种铜绿假单胞菌VITEK2 Compact分析仪法药敏结果的VME,MIE,CA相比较,差异有统计学意义(χ2=5.02,10.44,14.55,P<0.05)。黏液型铜绿假单胞菌VITEK2 Compact分析仪法药敏结果的CA比非黏液型铜绿假单胞菌低,且<90%,而VME,MIE均比非黏液型铜绿假单胞菌高。结论 VITEK2Compact全自动微生物分析仪对非黏液型铜绿假单胞菌药敏检测结果准确可靠,对黏液型铜绿假单胞菌药敏检测结果准确性较低,黏液型铜绿假单胞菌建议用其它方法做药物敏感性试验。
Abstract:
Objective To evaluate the accuracy of VITEK 2Compact automatic microbiology analyzer of antimicrobial susceptibility test formucoid Pseudomonas aeruginosa and non-mucoid Pseudomonas aeruginosa.Methods There were 29 strains mucoid Pseudomonas aeruginosa and 30 strains non-mucoid Pseudomonas aeruginosa collected from ahospital.The antimicrobial susceptibility test were detected by AGAR-dilutionmethod and VITEK2 Compact method respectively,and the AGAR-dilution method was adopted as the reference method for statistical analysis.ResultsFor mucoid Pseudomonas aeruginosa,compared to AGAR-dilution method,the rates of the complete accordances(CA),the very major errors(VME),themajor errors(ME),and the minor errors(MIE)were 84.69%,3.20%,2.13% and9.96%,respectively.For non-mucoid Pseudomonas aeruginosa,compared to AGAR-dilution method,the rates of the complete accordances(CA),the very majorerrors(VME),the major errors(ME),the minor errors(MIE)were 94.33%,0.67%,1.67% and 3.33%,respectively.Compared to two kinds of Pseudomonas aeruginosa,the rates of VME,MIE,CA were definded as statistical significance(χ2=5.02,10.44,14.55,P<0.05).The CA of the mucoid Pseudomonas aeruginosa was lower than that of non-mucoid Pseudomonas aeruginosa,while the VME,MIE of the mucoid Pseudomonas were higher than those of non-mucoid Pseudomonas aeruginosa.Conclusion For non-mucoid Pseudomonas aeruginosa,the antimicrobial susceptibility test results of VITEK 2 Compact are accurate and reliable,and for mucoid Pseudomonas aeruginosa,the accuracy of VITEK 2 Compact is lower,so that for mucoid Pseudomonas aeruginosa,the antimicrobial susceptibility test is suggested by other methoes.

参考文献/References:

[1] Clinical and Laboratory Standards Insitute.Perform-ance standards for antimicrobial susceptibity testing; Twentieth informational supplyment[S].Wayne:PA,CLSI document M100-S20,2014:61.
[2] Gherardi G,Angeletti S,Panitti M,et al.Comparati-ve evaluation of the Vitek-2 Compact and Phoenix systems for rapid identification and antibiotic susceptibility testing directly from blood cultures of Gram-negative and Gram-positive isolates[J].Diagn Microbiol Infect Dis,2012,72(1):20-31.
[3] 张保荣,臧志梅.黏液型铜绿假单胞菌感染实验鉴定与病例回顾分析[J].现代检验医学杂志,2015,30(2):114-116. Zhang BR,Zang ZM.Experimental identification of mucoid Pseudomonas aeruginosa strains causing infections and cases review[J].J Mod Lab Med,2015,30(2):114-116.
[4] 李瑜珍,曾学辉,李忠新,等.痰黏液型铜绿假单胞菌的分离及耐药性分析[J].中华医院感染学杂志,2014,24(5):1059-1060,1066. Li YZ,Zeng XH,Li ZX,et al.Isolation and resistance analysis of mucoid Pseudomonas aeruginosa from Sputum[J].Clin J Nosocomiol,2014,24(5):1059-1060,1066.
[5] 毕玲玲,孙成春,公衍文,等.黏液型和非黏液型铜绿假单胞菌的临床分布和耐药表型分析[J].解放军医学院学报,2015,36(2):140-143. Bi LL,Sun CC,Gong YW,et al.Clincal distribution and drug-resistant phenotype of mucoid Pseudomonas aeruginosa and non-mucoid Pseudomonas aeruginosa[J].Acad J Chin PLA Med Sch,2015,36(2):140-143.
[6] 朱伟斌,胡艳立,龙跃兵.黏液型与非黏液型铜绿假单胞菌的耐药性比较[J].中国感染控制杂志,2014,13(2):96-98. Zhu WB,Hu YL,Long YB.Comparison in antimicrobial resistance between mucoid and non-mucoid Pseudomonas aeruginosa[J].China J Infect Control,2014,13(2):96-98.
[7] 李晓燕,赵 杰,赵丽雅,等.黏液型铜绿假单胞菌耐药性分析[J].河北医药,2015,37(8):1247-1249. Li XY,Zhao J,Zhao LY,et al.The resistance analysis of mucoid Pseudomonas aeruginosa[J].Hebei Medical Journal,2015,37(8):1247-1249.
[8] 乔 宁,喻 华,殷 琳,等.VITEK 2 Compact全自动微生物分析仪性能分析[J].淮海医药,2012,30(3):211-213. Qiao N,Yu H,Yin L,et al.Performance analysis of VITEK 2 Compactantomatic microbiology analyzer[J].J Huaihai Med,2012,30(3):211-213.
[9] 周林涛,朱柏珍,刘晓云,等.侏儒型铜绿假单胞菌的鉴定[J].检验医学与临床,2012,9(14):1818-1819. Zhou LT,Zhu BZ,Liu XY,et al.Indentification of dwarf type Pseudomonas aeruginosa[J]. Lab Med Clin,2012,9(14):1818-1819.
[10] 蓝 锴,张伟铮,罗 强,等.不同方法检测黏液型铜绿假单胞菌药物敏感性结果准确性探讨[J].检验医学,2012,27(7):544-548. Lan K,Zhang WZ,Luo Q,et al.Accuracy evaluation of different antibioticsusceptibility testing of mucold isolates of Pseudomonas aeruginosa[J].Laboratary Medicine,2012,27(7):544-548.

相似文献/References:

[1]侯 欣a,张 丽a,徐英春a,等.念珠菌耐药机制研究进展[J].现代检验医学杂志,2016,31(01):60.[doi:10.3969/j.issn.1671-7414.2016.01.017]
　HOU Xina,ZHANG Lia,XU Ying-chuna,et al.Research Review on Antifungal Drug Resistance Mechanisms in Candida Spp[J].Journal of Modern Laboratory Medicine,2016,31(04):60.[doi:10.3969/j.issn.1671-7414.2016.01.017]
[2]吴红光,杨文东.围产期孕妇生殖道B族链球菌感染与耐药性分析[J].现代检验医学杂志,2016,31(01):104.[doi:10.3969/j.issn.1671-7414.2016.01.030]
　WU Hong-guang,YANG Wen-dong.Group B Streptococcus Infection in Vaginal Tract of Perinatal Pregnant Women and Drug Resistance Analysis[J].Journal of Modern Laboratory Medicine,2016,31(04):104.[doi:10.3969/j.issn.1671-7414.2016.01.030]
[3]邱晓明,林锦骠,黄 尔,等.黏液型与非黏液型铜绿假单胞菌Cif基因表达研究[J].现代检验医学杂志,2016,31(03):19.[doi:10.3969/j.issn.1671-7414.2016.03.006]
　QIU Xiao-ming,LIN Jin-piao,HUANG Er,et al.Investigation of Cif Gene Expression between Mucoid and Non-mucoid Pseudonmonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2016,31(04):19.[doi:10.3969/j.issn.1671-7414.2016.03.006]
[4]陈乔彬,刘爱胜,文 艳.深圳地区三级区属医院铜绿假单胞菌感染分布及耐药性分析[J].现代检验医学杂志,2015,30(03):147.[doi:10.3969/j.issn.1671-7414.2015.03.045]
　CHEN Qiao-bin,LIU Ai-sheng,WEN Yan.Infection Distribution and Drug Resistance Analysis of Pseudomonas Aeruginosa in Third District Hospital in Shenzhen Area[J].Journal of Modern Laboratory Medicine,2015,30(04):147.[doi:10.3969/j.issn.1671-7414.2015.03.045]
[5]多丽波,李桂玲,陈淑娟,等.铜绿假单胞菌金属酶及整合酶的检测[J].现代检验医学杂志,2015,30(01):34.[doi:10.3969/j.issn.1671-7414.2015.01.010]
　DUO Li-bo,LI Gui-ling,CHEN Shu-Juan,et al.Detection of Metallo-β-Lactamases and Integrasesin from Pseudomonas Aeruginosa Isolates[J].Journal of Modern Laboratory Medicine,2015,30(04):34.[doi:10.3969/j.issn.1671-7414.2015.01.010]
[6]罗史科,刘鲜花,朱平安,等.铜绿假单胞菌对耐喹诺酮药物质粒基因的研究[J].现代检验医学杂志,2015,30(01):39.[doi:10.3969/j.issn.1671-7414.2015.01.011]
　LUO Shi-ke,LIU Xian-hua,ZHU Ping-an,et al.Research on Pseudomonas Aeruginosa Quinolone-resistant Plasmid Genes[J].Journal of Modern Laboratory Medicine,2015,30(04):39.[doi:10.3969/j.issn.1671-7414.2015.01.011]
[7]谢国艳,高志生,许 俊,等.Cica-Beta Test试剂盒检测 耐亚胺培南铜绿假单胞菌金属β-内酰胺酶的评估[J].现代检验医学杂志,2015,30(01):123.[doi:10.3969/j.issn.1671-7414.2015.01.036]
　XIE Guo-yan,GAO Zhi-sheng,XU Jun,et al.Evaluation of Cica-Beta Test Kit for Detection of Metallo-β-Lactamase-Producing Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2015,30(04):123.[doi:10.3969/j.issn.1671-7414.2015.01.036]
[8]张河林,赵飞俊,何凤屏,等.2 527株临床分离病原菌的耐药性分析[J].现代检验医学杂志,2015,30(02):87.[doi:10.3969/j.issn.1671-7414.2015.02.027]
　ZHANG He-lin,ZHAO Fei-jun,HE Feng-ping,et al.Drug Resistance Spectrum Analysis of 2 527 Clinical Isolates of Bacteria[J].Journal of Modern Laboratory Medicine,2015,30(04):87.[doi:10.3969/j.issn.1671-7414.2015.02.027]
[9]吴友伟,石红,刘万静,等.腹膜透析液微生物培养病原学分析[J].现代检验医学杂志,2016,31(06):92.[doi:10.3969/j.issn.1671-7414.2016.06.026]
　WU You-wei,SHI Hong,LIU Wan-jing,et al.Bacteria Distribution and Drug Resistance Analysis of Peritoneal Dialysis Fluid[J].Journal of Modern Laboratory Medicine,2016,31(04):92.[doi:10.3969/j.issn.1671-7414.2016.06.026]
[10]谈 昀a,刘 萍,白永梅b.2011～2015年鲍曼不动杆菌耐药相关基因及耐药性变迁研究[J].现代检验医学杂志,2017,32(01):53.[doi:10.3969/j.issn.1671-7414.2017.01.015]
　TAN Yuna,LIU Ping,BAI Yong-meib.Study on the Drug Resistance Related Genes and Drug Resistance of Acinetobacter Baumannii from 2011 to 2015[J].Journal of Modern Laboratory Medicine,2017,32(04):53.[doi:10.3969/j.issn.1671-7414.2017.01.015]

备注/Memo

备注/Memo:

作者简介:李瑜珍(1972-),女,大学本科,学士学位,副主任技师,主要研究方向:临床微生物学,Tel:13828700966,E-mail:liyuzhen0803@163.com。
更新日期/Last Update: 2016-08-10