[1]苏显都a,范长玲a,于 莉a,等.血浆及单核细胞中microRNA表达水平与精神分裂症患者的相关性研究[J].现代检验医学杂志,2017,32(02):45-48，52.[doi:10.3969/j.issn.1671-7414.2017.02.012]
　SU Xian-dua,FAN Chang-linga,YU Lia,et al.Association of Epression with the Level of MicroRNA in Plasma and Mononuclear Cells in Patients with Schizophrenia[J].Journal of Modern Laboratory Medicine,2017,32(02):45-48，52.[doi:10.3969/j.issn.1671-7414.2017.02.012]
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血浆及单核细胞中microRNA表达水平与精神分裂症患者的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
45-48，52
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Association of Epression with the Level of MicroRNA in Plasma and Mononuclear Cells in Patients with Schizophrenia
文章编号:
1671-7414(2017)02-045-05
作者:
苏显都a; 范长玲a; 于 莉a; 李德芬b; 谢晋军b; 张素领a; 张小华a; 黄钰花b
儋州市人民医院a.检验科; b.精神科,海南那大 571799
Author(s):
SU Xian-dua; FAN Chang-linga; YU Lia; LI De-fenb; XIE Jin-junb; ZHANG Su-linga;  ZHANG Xiao-huaa; HUANG Yu-huab
a.Department of Clinical Laboratory; b.Department of Psychiatry,Danzhou People's Hospital,Hainan Nada 571799,China
关键词:
microRNA;  精神分裂症;  相关性
分类号:
R749.3; Q503
DOI:
10.3969/j.issn.1671-7414.2017.02.012
文献标志码:
A
摘要:
目的 探讨血浆及外周血单核细胞microRNA表达水平在精神分裂症患者中的变化及临床意义。方法 选取儋州市人民医院收治的174例精神分裂症患者作为病例组和80例健康体检者作为对照组。采用实时定量荧光PCR检测两组血浆和外周血单核细胞内8种microRNA(MiR-195,MiR-346,MiR-181b,MiR-212,MiR-30e,MiR-432,MiR-7和MiR-34a)的相对表达水平,并比较血浆和外周血单核细胞内microRNA的差异性。应用ROC曲线分析microRNA作为精神分裂症诊断标准的敏感度和特异度及Logistic回归分析microRNA判别精神分裂症的相对危险度。结果 病例组血浆MiR-195,MiR-181b,MiR-30e及MiR-7(3.11±1.05,2.18±0.72,1.85±0.74和9.61±1.87)表达水平较对照组(4.48±1.07,2.92±0.86,3.53±1.07和11.96±2.73)显著上调(P<0.05或P<0.01); 病例组外周血单核细胞MiR-181b,MiR-212,MiR-30e及MiR-34a(-4.20±1.16,0.27±0.55,-4.83±1.05和2.64±1.08)表达水平较对照组(-3.56±0.81,0.91±0.68,-3.49±1.22和3.95±1.03)显著上调(P<0.05或P<0.01)。Logistic回归分析显示,血浆MiR-181b及MiR-30e均具有显著相对危险度(OR=2.357,95%CI:1.361～4.093; OR=2.064,95%CI:1.147～3.815),外周血单核细胞MiR-30e也具有显著相对危险度(OR=1.628,95%CI:0.914～2.926)。ROC曲线分析显示,血浆及外周血单核细胞MiR-181b的AUC及95%CI分别为0.702(0.632～0.784),0.658(0.593～0.736); 血浆及外周血单核细胞MiR-30e的AUC及95%CI分别为0.775(0.706～0.857),0.758(0.686～0.839)。Spearman相关性分析显示,血浆MiR-181b与血浆MiR-30e呈明显相关(r=0.547,P=0.043)。结论microRNA在精神分裂症患者中异常表达,血浆及外周血单核细胞MiR-181b,MiR-30e对精神分裂症患者具有较好的诊断价值。
Abstract:
Objective To investigate the changes and clinical significance of the expression of microRNA in plasma and peripheral blood mononuclear cells in patients with schizophrenia.Methods 174 patients with schizophrenia in Danzhou People's Hospital were selected as thecase group and the other 80 healthy persons as control group.The relative expression levels of 8 microRNA in two groups of plasma and peripheral blood mononuclear cells were detected by real-time quantitative fluorescent PCR(MiR-195,MiR-346,MiR-181b,MiR-212,MiR-30e,MiR-432,MiR-7,MiR-34a),and the differences of microRNA in the plasma and peripheral blood mononuclear cells were compared between the two groups.ROC curve was used to analyze the sensitivity andspecificity of microRNA as diagnostic criteria for schizophrenia and Logistic regression analysis of the relative risk of microRNA in schizophrenia.Results The expression levels of MiR-195,MiR-181b,MiR-132,MiR-30e,MiR-7 and in the patients of the case group(3.11±1.05,2.18±0.72,1.85±0.74 and 9.61±1.87)were significantly higher than those in the control group(4.48±1.07,2.92±0.86,3.53±1.07 and 11.96±2.73,P<0.05or P<0.01).The expression levels of MiR-181b,MiR-212,MiR-30e and MiR-34a in peripheral blood mononuclear cells of patients in the case group(-4.20±1.16,0.27±0.55,-4.83±1.05 and 2.64±1.08)were significantly higher than those in control group(-3.56±0.81,0.91±0.68,-3.49±1.22 and 3.95±1.03,P<0.05 or P<0.01).Logistic regression analysis showed that plasma MiR-181b and MiR-30e were significantly relative risk(OR=2.357,95%CI:1.361～4.093; OR=2.064,95%CI:1.147～3.815),and peripheral blood mononuclear cells MiR-30e also had significant relative risk(OR=1.628,95%CI:0.914～2.926).ROC curve analysis showed that 95%CI and AUC in plasma and peripheral blood mononuclear cells of MiR-181b were 0.702(0.784～0.632),0.658(0.593 to 0.736),and plasma and peripheral blood mononuclear cell of MiR-30e were 0.775(0.706～0.857),0.758(0.686～0.839),respectively.Spearmancorrelation analysis showed that plasma MiR-181b and plasma MiR-30e were significantly correlated(r=0.547,P=0.043).Conclusion Abnormal expression of microRNA in patients with schizophrenia,and plasma andperipheral blood mononuclear cells MiR-181b and MiR-30e had good diagnostic value for schizophrenia patients.

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备注/Memo

备注/Memo:
基金项目:海南省医药卫生科研基金项目(15A200002)。
作者简介:苏显都(1988-),男,本科,检验师,从事临床检验诊断学研究,E-mial:adgbn129@126.com。
更新日期/Last Update: 2017-04-10