[1]张阳东,周建荣,楚瑞雪,等.血管紧张素Ⅱ1型受体3个单核苷酸多态性与冠心病的相关性分析[J].现代检验医学杂志,2017,32(02):57-59.[doi:10.3969/j.issn.1671-7414.2017.02.015]
　ZHANG Yang-dong,ZHOU Jian-rong,CHU Rui-xue,et al.Correlation between the Three Single Nucleotide Polymorphisms in Angiotensin Ⅱ Type one Receptor Gene and Coronary Artery Disease[J].Journal of Modern Laboratory Medicine,2017,32(02):57-59.[doi:10.3969/j.issn.1671-7414.2017.02.015]
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血管紧张素Ⅱ1型受体3个单核苷酸多态性与冠心病的相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
57-59
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Correlation between the Three Single Nucleotide Polymorphisms in Angiotensin Ⅱ Type one Receptor Gene and Coronary Artery Disease
文章编号:
1671-7414(2017)02-057-03
作者:
张阳东1; 周建荣2; 楚瑞雪3; 王利营1; 方 靓1
1.火箭军总医院特勤医学科,北京 100088;
2.解放军第532医院,安徽黄山 245000;
3.火箭军礼士路门诊部,北京 100820
Author(s):
ZHANG Yang-dong1; ZHOU Jian-rong2; CHU Rui-xue3; WANG Li-ying1; FANG Liang1
1.Department of Special Service Medicine,the General Hospitalof the PLA Rocket Force, Beijing 100088,China;
2.No.532 Hospital of the PLA,Anhui Huangshan 245000,China;
3.Lishilu Clinic of the PLA Rocket Force,Beijing 100820,China
关键词:
血管紧张素Ⅱ1型受体;  单核苷酸多态性;  冠心病;  单倍型
分类号:
R541.4; Q786
DOI:
10.3969/j.issn.1671-7414.2017.02.015
文献标志码:
A
摘要:
目的 观察中国汉族人群血管紧张素II 1型受体(AT1R)基因3个单核苷酸多态性(SNP)与冠心病(CAD)的相关性。方法 提取2011年1月～2013年10月就诊于火箭军总医院的192例CAD患者和189例健康对照者外周血白细胞DNA和RNA,设计引物,应用荧光标记单碱基延伸及寡核苷酸微阵列芯片杂交技术检测AT1R基因rs6801836,rs2675511和rs5182的基因型。结果 对照组和CAD组间3个SNP的基因型频率和等位基因频率差异无统计学意义(χ2=0.047～2.226,P值均>0.05); 由相互连锁的rs6801836和rs5182构成的单倍型以TT型为主,各单倍型频率在两组间差异无统计学意义(χ2=0.025～1.020,P值均>0.05)。结论 研究中AT1R基因的3个SNP与CAD的易感性无关。
Abstract:
Objective To investigate the association between the three single nucleotide polymorphisms(SNP)in angiotensin II type one receptor(AT1R)gene with coronary artery disease(CAD)in Chinese Han nationality.Methods Extracted DNA and RNA samples of peripheral blood white cells from 192 CAD patients and 189 healthy individuals in Jan 2011 toOct 2013 from the general hospital of the PLA Rocket Force.Designed primes andthe three SNPs as rs6801836,rs2675511 and rs5182 of AT1R gene were analyzed with allele-specific fluorogenic oligonucleotide probes in an assay combining extension and hybridization.Results The genotype and allelefrequencies of the three SNPs were not significantly different between the control group and CAD group(χ2=0.047～2.226,all P>0.05).The major haplotype constructed with linkaged rs6801836 and rs5182 was TT.The frequency of every haplotypes showed no significantly difference between the two groups(χ2=0.025～1.020,all P>0.05).Conclusion The three polymorphisms of AT1R gene studied in this work showed no association with CAD susceptibility.

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备注/Memo

备注/Memo:

作者简介:张阳东(1970-),男,医学博士,主任技师,研究方向:临床生物化学与分子生物学诊断,Tel:13671041699,E-mail: zhangyd262@sohu.com。
更新日期/Last Update: 2017-04-10