[1]邱 巍,胡 威,赵建江,等.江苏宿迁地区汉族人群DNMT3B基因启动子区-149C>T和-579G>T多态性与食管癌易感性的分析[J].现代检验医学杂志,2018,33(02):28-30.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　QIU Wei,HU Wei,ZHAO Jian-jiang,et al.Study on the Promoter Region -149C>T and -579G>T Polymorphism of DNMT3B with Genetic Susceptibility to Esophageal Cancer of Han Population in Jiangsu Suqian[J].Journal of Modern Laboratory Medicine,2018,33(02):28-30.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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江苏宿迁地区汉族人群DNMT3B基因启动子区-149C>T和-579G>T多态性与食管癌易感性的分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
28-30
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Study on the Promoter Region -149C>T and -579G>T Polymorphism of DNMT3B with Genetic Susceptibility to Esophageal Cancer of Han Population in Jiangsu Suqian
文章编号:
1671-7414(2018)02-028-03
作者:
邱 巍; 胡 威; 赵建江; 陈素梅; 刘东声
宿迁市人民医院检验科,江苏宿迁 223800
Author(s):
QIU Wei; HU Wei; ZHAO Jian-jiang; CHEN Su-mei; LIU Dong-sheng
Department of Clinical Laboratory,Suqian People's Hospital,Jiangsu Suqian 223800,China
关键词:
食管癌;  DNA甲基转移酶3B;  单核苷酸多态性
分类号:
R735.1; R730.43
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 分析江苏宿迁地区汉族人群DNA甲基转移酶3B(DNMT3B)基因启动子区-149C>T和-579G>T单核苷酸多态性(SNP)与食管癌(EC)易感性的关系。方法 利用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术分析246例食管癌患者及240例健康对照者的DNMT3B启动子区-149C>T和-579G>T位点的基因型。结果 在-149C>T位点中,食管癌组和对照组的基因型TT与CT比较差异无统计学意义(χ2=0.089,P>0.05)。在-579G>T位点中,食管癌组和对照组的基因型TT与GT+GG比较差异无统计学意义(χ2=0.649,P>0.05)。在年龄和性别分组中,两位点在食管癌组和对照组间基因型频率差异均无统计学意义(χ2=0.044～0.876,P值均>0.05)。结论 DNMT3B基因启动子区-149C>T和-579G>T与食管癌遗传易感性无相关性。
Abstract:
Abstract:Objective To investigate the association of DNAmethyltransferase 3B(DNMT3B)promoter region single nucleotide polymorphism(SNP)-149C>T and -579G>T with genetic susceptibility of esophageal cancer(EC)ofHan population in Jiangsu Suqian.Methods Genotypes of the -149C>T and -579G>T locus in DNMT3B promoter region were examined by polymerase chain reaction with restriction fragment length polymorphism(PCR-RFLP)in246 esophageal cancer patients and 240 healthy controls.Results In -149C>T site,the distributions of genotypes TT compared with CT inthe EC group and the controls were no significant difference(χ2=0.089,P>0.05).In -579G>T site,the distributions of genotypes TT compared with GT+GG in the EC group and the controls were no significant difference(χ2=0.649,P>0.05).When stratified by age and gender,there was no significant difference in the EC group and the controls in both two sites(χ2=0.044～0.876,all P>0.05).Conclusion The DNMT3B promoter region -149C>T and -579G>T were not associated with the genetic susceptibilityof esophageal cancer.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:江苏省宿迁市检验医学重点实验室项目(M201603)。 作者简介:邱 巍(1985-),男,硕士,主管检验师,主要从事生物化学与分子生物学检验研究,E-mail:qiuwei1117@163.com。 通讯作者:刘东声(1973-),男,硕士,主任检验师,主要从事分子遗传检验研究,E-mail:445781722@qq.com。
更新日期/Last Update: 2018-04-16