[1]肖小平,张 熊sub>a,秦光明b.食管癌和良性食管疾病患者血浆miRNA-21和miRNA-143检测的临床应用研究[J].现代检验医学杂志,2017,32(04):72-75,139.[doi:10.3969/j.issn.1671-7414.2017.04.020]
　XIAO Xiao-ping,ZHANG Xionga,QIN Guang-mingb.Clinical Research of Detecting Plasma MiRNA-21 and MiRNA-143 for Identifying Early Esophageal Cancer and Benign Esophageal Diseases[J].Journal of Modern Laboratory Medicine,2017,32(04):72-75,139.[doi:10.3969/j.issn.1671-7414.2017.04.020]
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食管癌和良性食管疾病患者血浆miRNA-21和miRNA-143检测的临床应用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
72-75,139
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Clinical Research of Detecting Plasma MiRNA-21 and MiRNA-143 for Identifying Early Esophageal Cancer and Benign Esophageal Diseases
文章编号:
1671-7414(2017)04-072-05
作者:
肖小平1; 张 熊<sub>2a; 秦光明2b
1.西安市第八医院检验科,西安 710061; 2.咸阳市第一人民医院 a.检验科; b.消化内科,陕西咸阳 712000
Author(s):
XIAO Xiao-ping1; ZHANG Xiong2a; QIN Guang-ming2b
1.Department of Clinical Laboratory,the Eighth Hospital of Xi'an City,Xi'an 710061,China; 2a.Department of Clinical Laboratory,2b.Department of Gastroenterology, the First People's Hospital of Xianyang City,Shaanxi Xianyang 712000,China
关键词:
食管癌;  良性食管疾病;  实时荧光定量PCR(RT-PCR);  miRNA-21;  miRNA-143
分类号:
R735.1; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.04.020
文献标志码:
A
摘要:
目的 探讨血浆微小RNA(microRNA,miRNA)-21,miRNA-143水平在鉴别早期食管癌和良性食管疾病中的临床应用。方法 收集27例早期食管癌患者(食管癌组)和25例良性食管疾病患者(良性食管疾病组)的血液及临床资料,并设立健康对照组,采用实时荧光定量PCR(real-time-PCR,RT-PCR)技术检测研究对象血浆miRNA-21,miRNA-143水平的表达,采用电化学发光技术检测血浆CEA和 CA72-4水平,探讨两标志物的变化与食管癌和良性食管疾病之间的关系。结果 食管癌组血浆miRNA-21,miRNA-143的表达量分别为0.93±0.17和0.27±0.05; 两标志物在良性食管疾病组的表达量分别为0.25±0.03和0.99±0.15; 两标志物在对照组的表达量分别为0.23±0.03和1.02±0.15。食管癌组miRNA-21,miRNA-143表达量分别与良性食管疾病组比较呈现上调、下调,差异有统计学意义(t=10.87,11.55,均P<0.01),与对照组比较呈现上调、下调,差异有统计学意义(t=9.20,9.07,均P<0.01)。良性食管疾病组血浆miRNA-21,miRNA-143表达量与对照组比较差异则无统计学意义(t=1.39,1.19,均P>0.05)。血浆miRNA-21,miRNA-143分别在食管癌组、良性食管疾病组和对照组的阳性率分别为81.5%(22/27),4.0%(1/25),0(0/24); 85.2%(23/27),4.0%(1/25),0(0/24)。食管癌组miRNA-21,miRNA-143阳性率分别与良性食管疾病组和对照组比较增高,差异有统计学意义(χ2=31.59,34.39,均P<0.01; χ2=34.42,37.23,均P<0.01 ),良性食管疾病组两标志物水平与对照组比较差异则无统计学意义(χ2=0.980,0.980,均P<0.01)。血浆miRNA-21,miRNA-143诊断早期食管癌的敏感度及特异度分别为81.4%,97.9%; 85.1%,97.9%。食管癌组miRNA-21,miRNA-143的灵敏度分别与CEA和 CA72-4比较明显增高,差异有统计学意义(χ2=12.79,P<0.01; χ2=5.33,P<0.05; χ2=15.03,P<0.01; χ2=6.95,P<0.05)。食管癌组miRNA-21,miRNA-143的特异度分别与CEA和CA72-4比较,差异无统计学意义(χ2=1.043,0.000,均P>0.05,χ2=1.043,0.000,均P>0.05)。采用Spearman相关性分析显示,在食管癌组中,血浆miRNA-21,miRNA-143表达量呈负相关(r=-0.658,P<0.01)。在该组中miRNA-21,miRNA-143 表达量分别与CEA和CA72-4水平也具有相关性(r=0.607,-0.623,均P<0.01; r=0.579,-0.610,均P<0.01)。结论 通过检测miRNA-21,miRNA-143水平在早期食管癌和良性食管疾病患者血浆中的表达,为早期食管癌的病理诊断提供新的思路。
Abstract:
Objective To explore the clinical significanceof detection of plasma microRNA-21,-143 in identifying early esophageal cancer and esophageal non-tumor diseases.Methods The expression of plasma microRNA-21,-143 in 27 cases of patients with early esophagealcancer(esophagus cancer group),25 cases of patients with non-esophageal tumor(non-esophageal tumor group)and in the healthy controls were detected by RT-PCR,and detected the levels of plasma CEA and CA72-4 by the electrochemical luminescence technology,which of changes were analysed to observe the relationship between the changes and the esophageal cancer,the benign esophageal diseasesfor the two markers.Results The expression of plasma microRNA-21,-143 in the esophagus cancer group were 0.93±0.17,0.27±0.05,which of ones in the non-esophagus cancer group were 0.25±0.03,0.99±0.15,and with those in the control group were 0.23±0.03,1.02±0.15.Comparedwith those in the non-esophagus cancer group,the expression of plasma microRNA-21,-143 were obviously up or down-regulated with significant differences(t=10.87,11.55,P<0.01).Compared with those in the control group,which of ones were obviously up or down-regulated with significant differences(t=9.20,9.07,P<0.01),and with no statistical significances in comparisonbetween the esophagus cancer group and the control group(t=1.39,1.19,P>0.05).The positiverate of plasma microRNA-21,-143 in the esophageal cancer,non-esophagus cancer group and the control group were,81.4%(22/27),4.0%(1/25)and 0(0/24); 85.1%(23/27),4.0%(1/25),and 0(0/24),respectively.The positive rate of microRNA-21,-143 in the esophageal group respectively incomparison with those in the non-esophagus cancer group and the control group were significantly higher,the differences had statistical significances(χ2=31.59,34.39,P<0.01; χ2=34.42,37.23,P<0.01).The expression of two markers in the esophagus cancer group were no statistically significant differences compared with control group(χ2=0.980,0.980,P>0.05).The sensitivity and specificity of microRNA-21,-143 in early diagnosis on the esophageal cancer were 81.4%,97.9% and 85.1%,97.9%.The sensitivity of microRNA-21,-143 in the esophageal group were significantly highercompared with those of CEA and CA72-4,the differences were statistically significant(χ2=12.79,P<0.01; χ2=5.33,P<0.05; χ2=15.03,P<0.01; χ2=6.95,P<0.05).The specificity of microRNA-21,-143 in the esophageal cancer group were no statistically significantdifferences in comparison with those of CEA and CA72-4(χ2=1.043,0.000,P>0.05)and(χ2=1.043,0.000,P>0.05),respectively.Theanalysis results from the spearman correlation test showed that in the esophageal cancer group,the expression of plasma microRNA-21,-143 had a negative correlation(r=0.658,P<0.01).Which of ones respectively associated with the levels of CEA and CA72-4(r= 0.607,0.623,P<0.01 and r=0.579,0.610,P<0.01).Conclution The detection of expression of plasma miRNA-21,miRNA-143 in the patients with the early esophageal cancerand non-esophageal tumor can provide a new train of thought for pathologic diagnosis of early esophageal cancer.

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备注/Memo

备注/Memo:
作者简介:肖小平(1968-),男,本科学历,主管检验师,研究方向:肿瘤标志物的检测,E-mail:418079040@qq.com。 通讯作者:张 熊(1968-),男,本科学历,副主任检验师,研究方向:食管疾病的诊断,E-mail:407205950@qq.com。
更新日期/Last Update: 1900-01-01