[1]李 勇,高春彪,盛伟武,等.儿童支气管哮喘患者血浆中miRNA-125b及miRNA-133b的表达及临床意义[J].现代检验医学杂志,2017,32(03):59-62.[doi:10.3969/j.issn.1671-7414.2017.03.016]
　LI Yong,GAO Chun-biao,SHENG Wei-wu,et al.Expression and Its Significance of microRNA-125b and microRNA-133b in Plasma in Children with Asthma[J].Journal of Modern Laboratory Medicine,2017,32(03):59-62.[doi:10.3969/j.issn.1671-7414.2017.03.016]
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儿童支气管哮喘患者血浆中miRNA-125b及miRNA-133b的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
59-62
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Expression and Its Significance of microRNA-125b and microRNA-133b in Plasma in Children with Asthma
文章编号:
1671-7 414(2017)03-059-04
作者:
李 勇1; 高春彪2; 盛伟武1; 管华玲3
1 .高邮市中医医院检验科，江苏高邮 225600；
2.常州市儿童医院检验科，江苏常州 213000；
3.盐城市第一人民医院检验科，江 苏盐城，224005
Author(s):
LI Yong1; GAO Chun-biao2; SHENG Wei-wu1; GUAN H ua-ling3
1．Department of Clinical Laboratory，Gaoyou Hosiptal of Traditional Chinese Medicine， Jiangsu Gaoyou 225600，China；
2.Department of Clinical Laboratory，Children’s Hospital of Changzhou，Jiangsu Changzhou 213000，China；
3.Department of Clinical Laborat
关键词:
支气管哮喘; miRNA-125b; miRNA-133b
分类号:
R725.7；R392.11
DOI:
10.3969/j.issn.1671-7414.2017.03.016
文献标志码:
A
摘要:
目的 探讨miRNA-125b和miRNA-133b在支气管哮喘患儿中 表达水平及其辅助诊断价值。方法 纳入2016年1月～11月常州市儿 童医院、高邮市中医医院30例哮喘患儿，分别收集其急性发作期和稳定期血液标本(A P组，SP组)。同期匹配30例变应性鼻炎患儿(AR组)及30例健康儿童为对照(NC组)。应用实时 荧光定量PCR检测miRNA在各组中的表达水平并相互比较。应用ROC曲线及AUC(95%CI)评估其 作为哮喘诊断指标的诊断效能。结果 miRNA-125b在AP组及SP组中 的表达量较AR组升高，差异有统计学意义(t=3.913，3.120，P值均＜0. 01)。miRNA -133b在AP组及AR组中的表达量较高，与NC组相比差异有统计学意义(t=4.426，4.720 ，P值均＜0.01)。miRNA-125b在哮喘诊断中最佳临界值为1.998，敏感度为64.52%， 特异度为92.67%，AUC为0.798 9，95%CI为0.711 1～0.886 4；miRNA-133b在哮喘急性 期及变应性鼻炎等气道炎症性疾病发作期中诊断最佳临界值为1.667，敏感度为58.06%， 特异度为86.67%，AUC为0.727 4，95%CI为0.586 5～0.863 0。结论 〖HT5 ”K〗哮喘患儿中miRNA-125b表达量较NC组及AR组升高，miRNA-125b可作为哮喘辅助诊断 指标，miRNA-133b可用于气道炎症性疾病发作期的辅助诊断。
Abstract:
Objective To explore the diagnostic value of th e quantitative detection of plasma miRNA-125b and miRNA-133b in children with asthma．Methods Thirty asthmatic patients were enrolled i n this study and collected the blood specimens during acute phase and stable pha se respectively (AP group and SP group)．Thirty allergic rhinitis children (AR g roup) and thirty healthy children were recruited to the control group (NC group) ．The levels of miRNA in different groups were detected by qRT-PCR．The perform ance of miRNA-125b and miRNA-133b were evaluated by receiver operating charact eristic curves (ROC) and the area under the curve (AUC) (95%CI)．Resu lts The relative expression of miRNA-125b in AP group and SP group were significantly higher than AR group (t=3.913，3.120，P＜0.01)，miRNA -133b．In AP group and AR group the expression of miRNA-133b were significantl y higher than control group (t=4.426，4.720，P＜0.01)．The detection of m iRNA-125b yielded an area under the curve of ROC of 0.7989，(95%CI：0.7111～ 0.8864) in discriminating asthmatic patients from healthy group． And the miRNA -133b was 0.7274 (95%CI：0.586 5～0.863 0) in discriminating asthmatic patie nts during the acute phase from healthy group．Conclusion 〖WT5”BZ 〗The relative expression of miRNA-125b in children with asthma was significant ly higher than that in AR group and NC group，miRNA-125b may prove to be a non -invasive biomarker for the auxiliary diagnosis of asthma especially when the r elative expression up to 1.998．

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备注/Memo

备注/Memo:

作者简介：李 勇(1973-)，男，大学本科，学士，副主任 技师，主要从事临床检验与研究，E-mail：59979133@qq．com。 高春彪(1971-)，男，大学本科，学士，副主任技师，主要从事临床免疫学检验与研究，E-mail：gcb2007@sina.com，共同第一作者。
通讯作者：盛伟武(1962-)，男，大学本科，副主任检验 师，主要从事临床检验与研究，E-mail：gysww@163.com。 管华玲(1974-)，女，学士，副
更新日期/Last Update: 2017-06-25