[1]李 洪,唐 浏,郑 淋,等.结肠癌组织中microRNA-126表达及临床意义[J].现代检验医学杂志,2017,32(04):60-63.[doi:10.3969/j.issn.1671-7414.2017.04.017]
 LI Hong,TANG Liu,ZHENG Lin,et al.Expression of microRNA-126 in Colon Cancer and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(04):60-63.[doi:10.3969/j.issn.1671-7414.2017.04.017]
点击复制

结肠癌组织中microRNA-126表达及临床意义()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
60-63
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Expression of microRNA-126 in Colon Cancer and Its Clinical Significance
文章编号:
1671-7414(2017)04-060-04
作者:
李 洪唐 浏郑 淋刘 娴
琼海市人民医院检验科,海南琼海 571400
Author(s):
LI HongTANG LiuZHENG LinLIU Xian
Department of Clinical Laboratory,Qionghai People's Hospital,Hainan Qionghai 571400,China
关键词:
结肠癌 microRNA-126 生物学功能
分类号:
R735.35; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.04.017
文献标志码:
A
摘要:
目的 探讨microRNA-126在结肠癌组织中的表达及生物学意义。方法 收集104例结肠癌手术患者,实时定量聚合酶链反应(RT-PCR)检测结肠癌患者的结肠癌组织以及癌旁组织中microRNA-126的表达(共104对)。应用慢病毒表达载体构建microRNA-126细胞系,并进一步通过体外实验研究microRNA-126对结肠癌细胞增殖、迁移和侵袭能力的影响。结果 结肠癌组织中microRNA-126相对表达水平(0.63±0.11)明显低于癌旁组织(1.08±0.15),差异有统计学意义(t=14.561,P<0.01)。结肠癌组织中microRNA-126表达阳性率(61.5%)显著低于癌旁组织(86.5%),差异有统计学意义(χ2=16.908,P<0.05)。microRNA-126表达与结肠癌Dukes分期、浸润深度、有无淋巴结转移及远处转移明显相关(P<0.05)。在有基质胶的Transwell实验中,转染组细胞迁移数(11.26±4.85个)明显低于空白对照组(264.37±32.15个),差异有统计学意义(t=23.418,P<0.01)。无基质胶的Transwell实验中,转染组细胞迁移数(83.75±13.74个)也明显低于空白对照组(339.64±26.38个),差异有统计学意义(t=12.682,P<0.01)。microRNA-126可以抑制SW480细胞的增殖及其侵袭转移。结论 microRNA-126可明显抑制结肠癌细胞的发生发展,影响结肠癌细胞的生物学行为。
Abstract:
Objective To investigate the expression and biological significance of microRNA-126 in colon cancer tissue.MethodsThe expression of microRNA-126 in colon cancer tissues and adjacent tissues of colon cancer patients was detected by real-time quantitative polymerase chain reaction(RT-PCR)in 104 patients with colon cancer surgery(totalof 104 pairs).The lentiviral vector was used to construct microRNA-126 cell line,and the effects of microRNA-126 on proliferation,migration and invasion ofcolon cancer cells were further studied in vitro.Results The relative expression of microRNA-126 in colon cancer tissues(0.63±0.11)was significantly lower than that in adjacent tissues(1.08±0.15),the difference was statistically significant(t=14.561,P<0.01).The positive rateof microRNA-126 expression in colon cancer tissues(61.5%)was significantly lower than that in adjacent cancer tissues(86.5%)the difference was statistically significant(χ2=16.908,P<0.05).The expression of microRNA-126 wassignificantly correlated with Dukes stage,depth of invasion,lymph node metastasis and distant metastasis(P<0.05).In the Transwell experiment with matrix glue,the number of cell migration in transfection group(11.26±4.85)wassignificantly lower than that in blank control group(264.37±32.15),the difference was statistically significant(t=23.418,P<0.01).In the Transwellexperiment without matrix glue,the number of cell migration in transfection group(83.75±13.74)was significantly lower than that in blank control group(339.64±26.38),the difference was statistically significant(t=12.682,P<0.01).MicroRNA-126 could inhibit the proliferation and invasion of SW480 cells.Conclusion MicroRNA-126 can significantly inhibit thedevelopment of colon cancer cells and affect the biological behavior of colon cancer cells.

参考文献/References:

[1] Peng F,Xiong L,Tang H,et al.Regulation of epithelial-mesenchymal transition through microRNAs:clinical and biological significance of microRNAs in breast cancer[J].Tumour Biol,2016,37(11):14463-14477.
[2] Pereira TC,Lopes-Cendes I.Emerging RNA-based drugs:siRNAs,microRNAs and derivates[J].Cent Nerv Syst Agents Med Chem,2012,12(3):217-232.
[3] Wu K,Zhao Z,Xiao Y,et al.Roles of mitochondrial transcription factorA and microRNA-590-3p in the development of colon cancer[J].Mol Med Rep,2016,14(6):5475-5480.
[4] Arora H,Qureshi R,Rizvi MA,et al.Study of apoptosis-related interactions in colorectal cancer[J].Tumour Biol,2016,37(11):14415-14425.
[5] Yuan W,Guo YQ,Li XY,et al.MicroRNA-126 inhibits colon cancer cell proliferation and invasion by targeting the chemokine(C-X-C motif)receptor 4 and Ras homolog gene family,member A,signaling pathway[J].Oncotarget,2016,7(37):60230-60244.
[6] Mohammadi A,Mansoori B,Baradaran B.The role of microRNAs in colorectalcancer[J].Biomed Pharmacother,2016,84(5):705-713.
[7] Zhang X,Shi H,Tang H,et al.miR-218 inhibits the invasion and migration of colon cancer cells by targeting the PI3K/Akt/mTOR signaling pathway[J].Int J Mol Med,2015,35(5):1301-1308.
[8] Zhang XF,Li KK,Gao L,et al.miR-191 promotes tumorigenesis of human colorectal cancer through targeting C/EBP β[J].Oncotarget,2015,6(6):4144-4158.
[9] Guo C,Sah JF,Beard L,et al.The noncoding RNA,miR-126,suppresses the growth of neoplastic cells by targeting phosphatidylinositol 3-kinase signaling and is frequently lost in colon cancers[J].Genes Chromosomes Cancer,2008,47(11):939-946.
[10] Osaki M,Oshimura M,Ito H.PI3K-Akt pathway:its functions andalterations in human cancer[J].Apoptosis,2004,9(6):667-676.
[11] Nie ZC,Weng WH,Shang YS,et al.MicroRNA-126 is down-regulated in human esophageal squamous cell carcinoma and inhibits the proliferation and migration in EC109 cell via PI3K/AKT signaling pathway[J].Int J Clin Exp Pathol,2015,8(5):4745-4754.
[12] Li N,Li X,Huang S,et al.miR-126 inhibits colon cancer proliferationand invasion through targeting IRS1,SLC7A5 and TOM1 gene[J].Journal of Central South University(Medical Science),2013,38(8):809-817.
[13] Ebrahimi F,Gopalan V,Wahab R,et al.Deregulation of miR-126 expression in colorectal cancer pathogenesis and its clinical significance[J].Exp Cell Res,2015,339(2):333-341.
[14] Li XM,Wang AM,Zhang J,et al.Down-regulation of miR-126 expressionin colorectal cancer and its clinical significance[J].Med Oncol,2011,28(4):1054-1057.
[15] Li Z,Li N,Wu M,et al.Expression of miR-126 suppresses migration andinvasion of colon cancer cells by targeting CXCR4[J].Mol Cell Biochem,2013,381(1/2):233-242.
[16] Li N,Tang A,Huang S,et al.MiR-126 suppresses colon cancer cell proliferation and invasion via inhibiting RhoA/ROCK signaling pathway[J].Mol CellBiochem,2013,380(1/2):107-119.
[17] Wang XY,Wu MH,Liu F,et al.Differential miRNA expression and their target genes between NGX6-positive and negative colon cancer cells[J].Mol Cell Biochem,2010,345(1/2):283-290.
[18] Hansen TF,S(¨overo)rensen FB,Lindebjerg J,et al.The predictive value of microRNA-126 in relation to first line treatment with capecitabine and oxaliplatin in patients with metastatic colorectal cancer[J].BMC Cancer,2012,8(3):1471-1477.

相似文献/References:

[1]芦 嘉,史伟峰.血清miRNA-720和miRNA-484在结肠癌分期及早期诊断中的应用[J].现代检验医学杂志,2017,32(01):77.[doi:10.3969/j.issn.1671-7414.2017.01.021]
 LU Jia,SHI Wei-feng.Application of Serum miRNA-720 and miRNA-484 in the Staging and Early Diagnosis of Colon Cancer[J].Journal of Modern Laboratory Medicine,2017,32(04):77.[doi:10.3969/j.issn.1671-7414.2017.01.021]
[2]吴 奇,申高飞,孙丽娜,等.miR-181d在结肠癌中表达失调并抑制癌细胞增殖及凋亡[J].现代检验医学杂志,2017,32(02):15.[doi:10.3969/j.issn.1671-7414.2017.02.004]
 WU Qi,SHEN Gao-fei,SUN Li-na,et al.miR-181d Inhibits Proliferation and Promotes Apoptosis in Colon Cancer Cells[J].Journal of Modern Laboratory Medicine,2017,32(04):15.[doi:10.3969/j.issn.1671-7414.2017.02.004]
[3]柏希慧,刘诗雨,孙媛媛.拉罗替尼通过AMPK/mTOR信号通路调控结肠癌细胞自噬和抑制增殖与迁移的实验研究[J].现代检验医学杂志,2024,39(06):29.[doi:10.3969/j.issn.1671-7414.2024.06.005]
 BAI Xihui,LIU Shiyu,SUN Yuanyuan.Experimental Study of Larotrectinib Regulating Autophagy and Inhibiting Proliferation and Migration of Colon Cancer Cells Through AMPK/mTOR Signaling Pathway[J].Journal of Modern Laboratory Medicine,2024,39(04):29.[doi:10.3969/j.issn.1671-7414.2024.06.005]

备注/Memo

备注/Memo:
作者简介:李 洪(1979-),男,本科,主管检验师,从事临床医学检验研究,E-mail:lihong668@126.com。
更新日期/Last Update: 1900-01-01