[1]张 悦a,段海霞,暴 蕾a,等.microRNA-125b在葛根素诱导人卵巢癌细胞SKOV3凋亡中的作用研究[J].现代检验医学杂志,2017,32(05):13-15,117.[doi:10.3969/j.issn.1671-7414.2017.05.004]
　ZHANG Yuea,DUAN Hai-xia,BAO Leia,et al.Role of MicroRNA-125b in Human Ovarian Cancer Cell SKOV3 Induced Apoptosis by Puerarin[J].Journal of Modern Laboratory Medicine,2017,32(05):13-15,117.[doi:10.3969/j.issn.1671-7414.2017.05.004]
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microRNA-125b在葛根素诱导人卵巢癌细胞SKOV3凋亡中的作用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
13-15,117
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Role of MicroRNA-125b in Human Ovarian Cancer Cell SKOV3 Induced Apoptosis by Puerarin
文章编号:
1671-7414(2017)05-013-04
作者:
张 悦1a; 段海霞2; 暴 蕾1a; 符乔珊1b
西安市第四医院a.妇产科; b.生殖科,西安 710004; 2.西北妇女儿童医院生殖妇科,西安 710056
Author(s):
ZHANG Yue1a; DUAN Hai-xia2; BAO Lei1a; FU Qiao-shan1b
1a.Department of Obstetrics and Gynecology; 1b.Department of Gynaecology,Xi'an Fourth Hospital,Xi'an 710004 China; 2.Department of Gynaecology,Northwest of Reproductive Women Children's Hospital,Xi'an710056,China
关键词:
卵巢癌;  葛根素;  microRNA-125b;  凋亡
分类号:
R737.31; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.05.004
文献标志码:
A
摘要:
目的 探讨micRNA-125b在葛根素诱导卵巢癌细胞SKOV3凋亡中的重要作用。方法 运用qRT-PCR技术检测葛根素处理卵巢癌细胞SKOV3中microRNA-125b的改变。运用干涉RNA技术抑制SKOV3细胞中microRNA-125b表达。运用Western blot技术检测SKOV3细胞microRNA-125b低表达后,葛根素处理组与对照组中凋亡相关蛋白的改变。结果 葛根素能够显著抑制卵巢癌细胞SKOV3的增殖活力以及促进其凋亡相关蛋白Cleaved Caspase-3,Bax和Bcl-2的表达,并且初步揭示了葛根素处理卵巢癌细胞SKOV3后能够促进microRNA-125b的表达; 抑制卵巢癌细胞SKOV3中microRNA-125b的表达能够降低葛根素对SKOV3细胞凋亡蛋白Cleaved Caspase-3,Bax和Bcl-2的表达。结论 microRNA-125b参与了葛根素对卵巢癌细胞SKOV3的促凋亡作用,提示microRNA-125b是卵巢癌细胞SKOV3耐药机制中的关键分子。
Abstract:
Abstract:Objective To explore whether puerarin induced apoptosis of SKOV3 ovarian cancer cells by microRNA-125b.Methods Using the qRT-PCR technique to detect the change of microRNA-125b afterpuerarin pretreated SKOV3.Using RNA interference technology to inhibit microRNA-125b expression in SKOV3 cells.Using Western blot technique to detect apoptosis related proteins after microRNA-125b lower expression.Results Puerarin could significantly inhibit ovarian cancer cell SKOV3 proliferation activity and promote its apoptosis related proteins expression.And puerarin can promote the expression of microRNA-125b.Inhibition of microRNA-125b expression in ovarian cancer cell SKOV3 could reduce apoptosis protein expression in SKOV3 cell.Conclusion MicroRNA-125b was involved in puerarin induced SKOV3 cell apoptosis,and prompt microRNA-125b is key molecular of the drug resistance in SKOV3.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年项目:Notch信号通路介导的神经干细胞自噬在铅神经毒性中的重要作用(81602815)。 作者简介:张 悦(1984-),女,硕士,主治医师,专业:妇科肿瘤,E-mail:304079774@qq.com, 通讯作者:符乔珊(1974-),女,硕士,主任医师,专业:妇科肿瘤,E-mail:157886964@qq.com。
更新日期/Last Update: 1900-01-01