[1]孙 林,胡三强,宋传涛.儿童感染性肺炎患者血液中炎症因子,ECP,EOS,IgE,VEGF及其受体检测与临床意义分析[J].现代检验医学杂志,2018,33(01):121-123,127.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　SUN Lin,HU San-qiang,SONG Chuan-tao.Detection of Inflammatory Factors,ECP,EOS,IgE,VEGF and Its Receptors in Children with Infectious Pneumonia[J].Journal of Modern Laboratory Medicine,2018,33(01):121-123,127.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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儿童感染性肺炎患者血液中炎症因子,ECP,EOS,IgE,VEGF及其受体检测与临床意义分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
121-123,127
栏目:
论著
出版日期:
2018-02-05

文章信息/Info

Title:
Detection of Inflammatory Factors,ECP,EOS,IgE,VEGF and Its Receptors in Children with Infectious Pneumonia
文章编号:
1671-7414(2018)01-121-04
作者:
孙 林; 胡三强; 宋传涛
连云港市妇幼保健院检验科,江苏连云港 222000
Author(s):
SUN Lin; HU San-qiang; SONG Chuan-tao
Department of Clinical Laboratory, Maternal and Child Health Hospital of Lianyungang,Jiangsu Liangyungang 222000,China
关键词:
感染性肺炎患儿;  炎症因子;  嗜酸性粒细胞;  免疫球蛋白E;  血管内皮生长因子
分类号:
R725.6; R392.11
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 分析肿瘤坏死因子-α(TNF-α)、白细胞介素-5(IL-5)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、嗜酸性细胞阳离子蛋白(ECP)、嗜酸性粒细胞(EOS)、免疫球蛋白E(IgE)、血管内皮生长因子(VEGF)及其受体在感染性肺炎患儿血清中检测的临床意义。方法 选择2016年3月～2017年3月来连云港市妇幼保健院就诊的80例感染性肺炎患儿,将其纳入观察组,按照病原体差异,分为病毒感染组、支原体感染组及细菌感染组。同时选择30例健康儿童作为对照组。采用全自动血细胞分析仪测定各组受试者血液中EOS数量,酶联免疫吸附测定法(ELISA)检测血清中TNF-α,IL-5,IL-6,IL-8,ECP,VEGF,VEGF-R1和VEGF-R2的水平,免疫散射比浊法测定血清中IgE的水平。结果 观察组患儿血液中TNF-α,IL-5,IL-6,IL-8,ECP,VEGF,VEGF-R1,VEGF-R2,EOS及IgE的水平显著高于对照组儿童,差异均有统计学意义(t=2.325～3.593,P<0.05)。细菌感染组患儿血清中的TNF-α,IL-5,IL-6和IL-8水平显著高于病毒感染组及支原体感染组,差异有统计学意义(P<0.05)。支原体感染组患儿血清中的VEGF,VEGF-R1和VEGF-R2水平则显著高于细菌感染组与病毒感染组,差异有统计学意义(P<0.05)。血清IgE水平及外周血中EOS数量在细菌感染组、病毒感染组及支原体感染组中的差异无统计学意义(P>0.05)。结论 血清TNF-α,IL-5,IL-6和IL-8可作为细菌感染性肺炎与病毒感染性肺炎及支原体感染性肺炎的鉴别指标。
Abstract:
Abstract:Objective To analyse the clinical significanceof(IL-5),interleukin-6(IL-6),interleukin-8(IL-8),eosinophil cationicprotein(ECP),eosinophil(EOS),immunoglobulin E(IgE),vascular endothelial growth factor(VEGF)and its receptor in serum of children with infectious pneumonia.Methods 80 children with infectious pneumonia were enrolled in Lianyungang Maternal and Child Health Hospital from March 2016 to March 2017,and were included in the observation group.According to the pathogen type,these children in the observation group were divided into virus group,mycoplasma infection group and bacterial infection group.At the same time,30 healthy children were selected as the control group.The levels of TNF-α,IL-5,IL-6,IL-8,ECP,VEGF,VEGF-R1 and VEGF-R2 in serum were measured by enzyme-linked immunosorbent assay(ELISA).The levels of EOS in blood were measured by automatic blood cell analyzer and the level of lgE in serum was detected by immunoturbidimetric turbidimetric assay.Results The levels ofTNF-α,IL-5,IL-6,IL-8,ECP,VEGF,VEGF-R1,VEGF-R2,EOS and IgE in theblood of the observation group were significantly higher than those in the control group(t=2.325～3.593,all P<0.05).The levels of TNF-α,IL-5,IL-6 and IL-8 in the serum of the bacterial infection group were significantlyhigher than those in the virus group and mycoplasma group(all P<0.05).The levels of VEGF,VEGF-1 and VEGF-R2 in serum of patients with mycoplasma infection were significantly higher than those in the group of bacterial infection and virus infection(all P<0.05).Serum IgE levels and the number of peripheral blood EOS in the bacterial infection group,viral infection group and mycoplasma infection group had no significant difference(P>0.05).Conclusion The serum levels of TNF-α,IL-5,IL-6 and IL-8 can beused as markers for bacterial infectious pneumonia and viral infectious pneumonia and mycoplasma infectious pneumonia.

参考文献/References:

[1] 许姜姜,舒林华,钟海琴,等.不同感染类型的儿童社区获得性肺炎流行病学特征研究[J].现代预防医学,2016,43(16):2909-2912. Xu JJ,Shu LH,Zhong HQ,et al.An epidemiological study of different types of infection among children with community-acquired pneumonia[J].Modern Preventive Medicine,2016,43(16):2909-2912.
[2] 金 姐,王立波,张明智,等.378例儿童支原体肺炎的临床特点及纤维支气管镜治疗[J].重庆医学,2015,44(34):4815-4817. Jin J,Wang LB,Zhang MZ,et al.Clinical features and bronchoscopy treatment for 378 patients of mycoplasma pneuoniae pneumonia in children[J].Chongqing Medicine,2015,44(34):4815-4817.
[3] 尹玉东,曲久鑫,杨春霞,等.国内外社区获得性肺炎中肺炎支原体检出率及耐药性不同原因分析[J].中国实用内科杂志,2016,36(8):721-723. Yin YD,Qu JX,Yang CX,et al.Analysis of the di-fference of the detection rete and resistance of Mycoplasma pneumoniae in our country and other countries[J].Chinese Journal of Practical Internal Medicine,2016,36(8):721-723.
[4] 许有才,赵世元.IL-3在肺炎支原体感染小鼠体内的表达及相关机制[J].现代检验医学杂志,2015,30(4):102-104. Xu YC,Zhao SY.Expression of interleukin-3 and their mechanisms inmice infected by Mycoplasma pneumoniae[J].Journal of Modern Laboratory Medicine,2015,30(4):102-104.
[5] Zhou J,Ren L,Chen D,et al.IL-17B is elevated in patients with pneumonia and mediates IL-8 production in bronchial epithelial cells[J].Clinical Immunology,2017(175):91-98.
[6] 马志超,黄白丽,阮和球,等.降钙素原联合呼吸道病原体抗体检测在小儿肺炎的诊断价值[J].现代检验医学杂志,2015,30(2):144-145,148. Ma ZC,Huang BL,Ruan HQ,et al.Diagnostic value of PCT combined with respiratorypathogens detection in pneumonia in children[J].Jo-urnal of Modern Laboratory Medicine,2015,30(2):144-145,148.
[7] 徐祖龙,董苏荣,张亚明.WBC,CRP和ESR联合检测在肺炎支原体肺炎中的意义[J].现代检验医学杂志,2015,30(1):153-155,158. Xu ZL,Dong SR,Zhang YM,et al.Significance of combining with WBC,CRP and ESR detection in patients with Mycoplasma pneumoniae pneumonia[J].Journal of Modern Laboratory Medicine,2015,30(1):153-155,158.
[8] 宋 明,赵芝娜,徐慰倬.肺炎支原体感染实验室检测的研究进展[J].中国感染控制杂志,2016,15(11):887-893. Song M,Zhao ZN,Xu WZ.Advances in laboratory detection of Mycoplasma pneumoniae infection[J].Chinese Journal of Infection Control,2016,15(11):887-893.
[9] 方慧英,周均华,张连丰,等.感染性肺炎新生儿体液与细胞免疫指标的变化分析[J].中华医院感染学杂志,2016,26(17):4058-4060. Fang HY,Zhou JH,Zhang LF,et al.Changes of humoral immunity and cellular immunity indexes of neonates with infectious pneumonia[J].Chinese Journal of Nosocomiology,2016,26(17):4058-4060.
[10] Wang L,Chen Q,Shi C,et al.Changes of serum TN F-α,IL-5 and IgE levels in the patients of Mycoplasma pneumonia infection with orwithout bronchial asthma[J].International Journal of Clinical & ExperimentalMedicine,2015,8(3):3901-3906.
[11] 温建英.感染性肺炎患儿血清IL-5和免疫球蛋白检测结果分析[J].医学理论与实践,2016,29(16):2247-2248. Wen JY.The detection of serum IL-5 and immunoglobulin in children with infectious pneumonia[J].Journal of Medical Theory and Practice,2016,29(16):2247-2248.
[12] 李新玲,吴振国,朱颀峰.慢性阻塞性肺疾病急性加重期血清白介素-6表达变化与肺炎衣原体感染的相关性[J].中华医院感染学杂志,2015,25(2):247-249. Li XL,Wu ZG,Zhu QF.Correlation between change of expression of serum interleukin-6 and Chlamydia pneumoniae infections in patients with acute exacerbation of chronic obstructive pulmonary disease[J].Chinese Journal of Nosocomiology,2015,25(2):247-249.
[13] 李 梅,陈正立,徐茜茹,等.肺炎支原体肺炎患儿血清中IL-5,IL-18和TNF-α检测及与病情严重程度的研究[J].现代预防医学,2015,42(14):2661-2662,2674. Li M,Chen ZL,Xu QR,et al.The detection of serum IL-5,IL-18 and TNF-α and disease severity in children with Mycoplasma pneumonia infection[J].Modern Preventive Medicine,2015,42(14):2661-2662,2674.
[14] Cheng HJ,Qiao HM,Liu L,et al.Clinical significance of IL-5 and VEGFin BALF with Mycoplasma pneumoniae pneumonia[J].Paediatric Respiratory Reviews,2012,13(Suppl 1):S61.
[15] 朱湘芸,王 洵,赵弘卿.支气管哮喘患儿血清TNF-α,IgE水平变化及其与肺炎支原体感染的关系[J].山东医药,2017,57(1):79-80. Zhu XY,Wang X,Zhao HQ.Changes of serum TN F-α and IgE levels in children with bronchial asthma and its relationship with Mycoplasma pneumoniae infection[J].Shandong Medicine,2017,57(1):79-80. 收稿日期:2017-07-19 修回日期:2017-09-12

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备注/Memo

备注/Memo:
作者简介:孙 林(1973-),女,本科,副主任技师,主要从事医学生物化学检验工作,E-mail:sunwzx0730@163.com。
更新日期/Last Update: 2018-02-07