[1]谢国艳,肖 敏.铜绿假单胞菌D-试验阳性及阴性菌株产β-内酰胺酶的差异性分析[J].现代检验医学杂志,2018,33(03):130-132.[doi:10.3969/j.issn.1671-7414.2018.03.033]
　XIE Guo-yan,XIAO Min.Study on the Difference of β-Lactamases-Producing in D-Test Positive and D-Test Negative Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2018,33(03):130-132.[doi:10.3969/j.issn.1671-7414.2018.03.033]
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铜绿假单胞菌D-试验阳性及阴性菌株产β-内酰胺酶的差异性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年03期
页码:
130-132
栏目:
研究简报·实验技术
出版日期:
2018-07-17

文章信息/Info

Title:
Study on the Difference of β-Lactamases-Producing in D-Test Positive and D-Test Negative Pseudomonas Aeruginosa
文章编号:
1671-7414(2018)03-130-03
作者:
谢国艳1; 肖 敏2
1.上海交通大学医学院附属新华医院崇明分院检验科,上海 202150; 2.解放军第306医院医学检验科,北京 100101
Author(s):
XIE Guo-yan1; XIAO Min2
1.Department of Clinical Laboratory,Chongming Branch of Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 202150,China; 2.Department of Clinical Laboratory,the 306th Hospital of PLA,Beijing 100101,China
关键词:
铜绿假单胞菌;  β-内酰胺酶;  D试验
分类号:
R378.991; R446.5
DOI:
10.3969/j.issn.1671-7414.2018.03.033
文献标志码:
A
摘要:
目的 探讨D-试验阳性现象的铜绿假单胞菌(PA)的发生率及其现象的发生机制。方法 使用纸片扩散法(K-B法)检测386株PA对亚胺培南和头孢他啶的耐药性,对D-试验阳性及阴性株分别检测AmpC和金属β-内酰胺酶(MBLs)。结果 386株PA分离株中,有132株为D-试验阳性株,D-试验阳性的PA发生率为34.2%,总产酶率为73.5%,其中MBLs占40.1%,AmpC酶占30.3%,混合酶占3.0%。D-试验阴性株总产酶率为9.0%,其中MBLs占3.0%,AmpC酶占6.0%。两者在总产酶率、产MBLs和产AmpC酶方面的差异具有统计学意义(χ2=39.9,26.4,14.7,均P<0.01)。其中98株(74.2%)菌株为D1型(亚胺培南耐药),34株(25.8%)菌株为D2型(亚胺培南敏感),D1型与D2型相比较,在产MBLs和产AmpC酶方面的差异具有统计学意义(χ2=6.1,3.9,均P<0.05)。结论 实验室应高度重视D-试验阳性的PA株的检测。推测PA株D-试验阳性现象的产生可能与MBLs和AmpC酶的大量产生有关。
Abstract:
Abstract:Objective To investigate the incidence rate ofD-test-positive phenomenon and the mechanism of the phenomenon-producing inPseudomonas aeruginosa(PA).Methods 386 PA clinicalisolates were dectected to drug susceptibility of imipenem and ceftazidime by K-B method,and D-test positive and negative trains were dectected to AmpC enzymes and MBLs,respectively.Results There were 132 D-test-positive trains in 386 PA clinical isolates,the incidence rate of D-test-positive trains was 34.2%.In D-test-positive trains,total enzymes-producingrate was 73.5%,and MBLs,AmpC enzymes and MBLs+AmpC enzymeswas were 40.1%,30.3% and 3.0%,respectively.In D-test-negative trains,total enzymes-producing rate was 9.0%,and MBLs,AmpC enzymes were 3.0% and 6.0%,respectively.Compared withtotal enzymes-producing rate,AmpC enzymes and MBLs,D-test-positive trains had significant difference with negative ones(χ2=39.9,26.4,14.7,all P<0.01).D1-type trains were 74.2% and D2-type trains were25.8%.Compared with MBLs and AmpC enzymes,D1-type trainshad significant difference with D2-type trains(χ2=6.1,3.9,all P<0.05).Conclusion Clinical microbiological laboratory should highly pay attention to the detection in D-test-positive-PA strains.The production of MBLs and AmpC enzymes in great amount induced are probablely related to the phenomenon-producing in D-test-positive PA.

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备注/Memo

备注/Memo:
作者简介:谢国艳(1974-),女,硕士,副主任技师,研究方向:主要从事医学检验与耐药基因分子诊断研究,E-mail:xieguoyan93@163.com。 通讯作者:肖 敏,女,博士,副主任技师,E-mail:gaozhishen93@163.com。
更新日期/Last Update: 2018-04-16