[1]谷钰峰,李 昱,张晓录,等.基于MALDI-TOFMS与FCM联合应用对临床血流感染病原体鉴定和药敏试验新方法的建立和应用[J].现代检验医学杂志,2018,33(04):2-24.[doi:10.3969/j.issn.1671-7414.2018.04.006]
 GU Yu-feng,LI Yu,ZHANG Xiao-lu,et al.A New Method Aimed to Quickly Identify Pathogen and Drug Susceptibility Test Based on MALDI-TOF MS Combined with FCM[J].Journal of Modern Laboratory Medicine,2018,33(04):2-24.[doi:10.3969/j.issn.1671-7414.2018.04.006]
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基于MALDI-TOFMS与FCM联合应用对临床血流感染病原体鉴定和药敏试验新方法的建立和应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
2-24
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
A New Method Aimed to Quickly Identify Pathogen and Drug Susceptibility Test Based on MALDI-TOF MS Combined with FCM
文章编号:
1671-7414(2018)04-020-05
作者:
谷钰峰李 昱张晓录黄葆华孙成铭
烟台毓璜顶医院检验中心,山东烟台 264000
Author(s):
GU Yu-fengLI YuZHANG Xiao-luHUANG Bao-huaSUNCheng-ming
Center of Clinical Laboratory, Yantai Yuhuangding Hospital,Shandong Yantai 264000,China
关键词:
血流感染 基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF MS) 流式细胞学(FCM) 快速鉴定 药物敏感实验
分类号:
R378; Q503
DOI:
10.3969/j.issn.1671-7414.2018.04.006
文献标志码:
A
摘要:
目的 探讨基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF MS)与流式细胞学(FCM)联合应用在临床血流感染(BSI)病原体快速鉴定和药敏试验中的意义及应用价值。方法 采用分离胶-吸附法菌体分离系统直接将菌体从血培养阳性瓶中分离,通过流式细胞学(FCM)与基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF MS)联合应用,快速对病原体进行鉴定和药敏试验。结果MALDI-TOF MS法和VITEK2系统对常见菌都有超过90%的鉴定到种的能力(t=12.70,P=0.213)。MALDI-TOF MS较VITEK2有更高的鉴定到群和属的能力(t=6.31,P=0.027; t=8.64,P=0.031)。FCM法的药敏结果与VITEK2系统结果几乎完全一致(t=4.30,P=0.127),且FDA的加入可以很好地发现感染菌群中的耐药异质性。结论 联合应用MALDI-TOF MS与FCM对临床BSI病原体进行鉴定和药敏试验的结果与传统方法间有很好的一致性,且报告时间可由传统的36,72 h缩短至3 h以内。该方法对BSI的快速诊断有较大意义和较好的临床应用价值。
Abstract:
Abstract:Objective To discuss the significance and applied value in the rapid identification and drug susceptibility test for bloodstream infection(BSI)using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS)combined with flow cytometry(FCM).Methods The bacteria were separated from the positive blood culture bottle using separation gel-adsorption method system,and then applyed MALDI-TOF MS combined with FCM to identify pathogen and drug susceptibility testquickly.Results The efficiency of the separation gel-adsorption method for gram-negative bacterium,gram-positive bacteria and fungiwere 71%,74% and 88% separately.The results of identifying pathogen using MALDI-TOF MS accord with results using VITEK2,both methods could identify 90% bacteria to species(t=12.70,P=0.213).The MALDI-TOF MS had higher ability to identify groups and genera than VITEK2(t=6.31,P=0.027; t=8.64,P=0.031).The results of drug susceptibility test using FCM was practically identical to VITEK2(t=4.30,P=0.127),besides,the addition of FDA could findthe heterogenic drug-resistant strains well.Conclusion They could quickly identify pathogen and drug susceptibility test based on MALDI-TOF MS combined with FCM,which was consistent with the traditional methodsand could shorten the report time from 36,72 h to 3 h.More importantly,this methods are of great significance and clinical importance for the rapid identification of bloodstream infection.

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备注/Memo

备注/Memo:
作者简介:谷钰峰(1987-),男,硕士,技师,主要从事微生物致病机制研究,E-mail:guyufeng87@hotmail.com。 通讯作者:孙成铭(1972—),男,博士,主任技师,主要从事临床分子生物学诊断研究,E-mail:18953569897@163.com。
更新日期/Last Update: 2018-08-16