[1]金秀华,李艳冬.TIM-3基因启动子区-1516G/TRs153538和外显子区-882C/TRs474853,-574T/GRs151746位点多态性与蒙古族儿童哮喘的关系研究[J].现代检验医学杂志,2018,33(04):73-75/8.[doi:10.3969/j.issn.1671-7414.2018.04.019]
　JIN Xiu-hua,LI Yan-dong.Relationship between TIM-3 Gene Promoter Region -1516G/T (Rs10053538),Exon Region -882C/T(Rs4704853),-574T/G(Rs1051746) Polymorphisms and Asthma of Mongolian Nationality Children[J].Journal of Modern Laboratory Medicine,2018,33(04):73-75/8.[doi:10.3969/j.issn.1671-7414.2018.04.019]
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TIM-3基因启动子区-1516G/TRs153538和外显子区-882C/TRs474853,-574T/GRs151746位点多态性与蒙古族儿童哮喘的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
73-75/8
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Relationship between TIM-3 Gene Promoter Region -1516G/T (Rs10053538),Exon Region -882C/T(Rs4704853),-574T/G(Rs1051746) Polymorphisms and Asthma of Mongolian Nationality Children
文章编号:
1671-7414(2018)04-073-04
作者:
金秀华1; 李艳冬2
1.赤峰市蒙医中医医院检验科,内蒙古赤峰 024000; 2.赤峰市红山区妇幼保健所检验科,内蒙古赤峰 024000
Author(s):
JIN Xiu-hua1; LI Yan-dong2
1.Department of Clinical Laboratory, the Mongolian Medicine Hospital of Traditional Chinese Medicine of Chifeng City,Inner Mongolia Chifeng 024000,China; 2.Department of Clinical Laboratory,theHongshan District Health Center for Women and Children of Chif
关键词:
T细胞免疫球蛋白黏蛋白域3;  单核苷酸多态性;  儿童哮喘
分类号:
R725.6; Q786
DOI:
10.3969/j.issn.1671-7414.2018.04.019
文献标志码:
A
摘要:
目的 探讨哮喘免疫调节基因T细胞免疫球蛋白黏蛋白域3(Tim-3)启动子区多态性位点-1516G/T(Rs10053538)和外显子区多态性位点-882C/T(Rs4704853),-574T/G(Rs1051746)与内蒙古地区蒙古族儿童哮喘的遗传易感性。方法收集赤峰市蒙医中医医院和红山区妇幼保健所2014年6月～2016年12月哮喘组儿童129例,平均年龄6.2岁; 正常体检对照组儿童130例,平均年龄6.0岁。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测所有研究对象的三个多态性位点的基因型,进行病例对照研究分析。结果 哮喘组-574T/G(Rs1051746)位点基因多态性的T等位基因明显高于对照组,差异有统计学意义(OR=0.239,95%CI 0.067～0.858,P<0.05)。哮喘组Tim-3启动子区-1516G/T(Rs10053538)位点基因多态性与对照组比较OR=0.835,95%CI 0.371～1.883,P=0.664; 哮喘组外显子-882C/T(Rs4704853)位点基因多态性与对照组比较,差异无统计学意义(OR=0.326,95%CI0.33～3.172,P=0.310)。结论 Tim-3基因-574T/G(Rs1051746)位点与蒙古族儿童哮喘易感性相关,为内蒙古地区儿童哮喘的发病机制研究提供参考。
Abstract:
Abstract:Objective To elucidate whether the polymorphisms within the asthma immune regulator gene T cell immunoglobulin domain and mucindomain 3 two polymorphism loci,-882C/T(Rs4704853),-574T/G(Rs1051746)at exon region and one polymorphism loci,-1516G/T(Rs10053538)at promoter region were associated with the risk of Mongolian nationality childhood asthma.Methods Polymerase chain reaction(PCR)was used to test the genotypes of three polymorphism loci among 129 cases(average 6.2y)of asthma and 130 controls(average 6.0y)in a case-control study collected from June of 2014to June of 2016.Results The results of PCR-RFLP showedthat the three polymorphisms in TIM-3 gene were present in the study population.TIM-3 -574T/G(Rs1051746)polymorphism was associated with Mongolian nationality childhood asthma(OR=0.239,95%CI 0.067～0.858,P<0.05),but at promoter region -1516G/T(Rs10053538)polymorphism(OR=0.835,95%CI 0.371～1.883,P=0.664)and -882C/T(Rs4704853)at exon region(OR=0.326,95%CI 0.33～3.172,P=0.310)were not.Conclusion These results indicated that -574T/G(Rs1051746)polymorphism of the TIM-3 gene was associated with Mongolian nationality childhood asthma,providing a better understanding of the pathogenesis of the allergic asthma among children in Inner Mongolia.

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备注/Memo

备注/Memo:
作者简介:金秀华(1972-),女,本科,副主任检验师,检验科主任,主要从事哮喘基因多态性基础研究,E-mail:1813214327@qq.com。
更新日期/Last Update: 2018-08-16