[1]周朝琼,邓 劲,刘 翔,等.重症监护室患者耐碳青霉烯类肺炎克雷伯菌流行病学研究及同源性分析[J].现代检验医学杂志,2019,34(04):71-74.[doi:10.3969/j.issn.1671-7414.2019.04.017]
　ZHOU Chao-qiong,DENG Jin,LIU Xiang,et al.Epidemiological Study and Homology Analysis of Carbapenem-Resistant Klebsiella Pneumoniae in Intensive Care Unit Patients[J].Journal of Modern Laboratory Medicine,2019,34(04):71-74.[doi:10.3969/j.issn.1671-7414.2019.04.017]
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重症监护室患者耐碳青霉烯类肺炎克雷伯菌流行病学研究及同源性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
71-74
栏目:
论著
出版日期:
2019-08-20

文章信息/Info

Title:
Epidemiological Study and Homology Analysis of Carbapenem-Resistant Klebsiella Pneumoniae in Intensive Care Unit Patients
文章编号:
1671-7414(2019)04-071-04
作者:
周朝琼1; 邓 劲2; 刘 翔1; 孔丽蕊1
[1.成都市郫都区中医医院(成都中医药大学附属第三医院)检验科,成都 611730; 2.四川大学华西医院实验医学科,成都 610041]
Author(s):
ZHOU Chao-qiong1; DENG Jin2; LIU Xiang1; KONG Li-rui1
[1.Department of Clinical Laboratory,Chinese Medicine Hospital of Pidu(the Third Affiliated Hospital of Chengdu Univisity of Traditional Chinese Medicine),Chengdu 611730,China; 2.Department of Clinical Laboratory,West China Hospital of Sichuan University,Chengdu 610041,China]
关键词:
重症监护室患者;  耐碳青霉烯类肺炎克雷伯菌;  流行病学研究;  同源性分析
分类号:
R378.996; R181.32
DOI:
10.3969/j.issn.1671-7414.2019.04.017
文献标志码:
A
摘要:
目的 对重症监护室患者分离出的耐碳青霉烯类肺炎克雷伯菌进行流行病学研究,为临床治疗及监测提供参考依据。方法 收集2018年1月～2019年1月成都市郫都区中医医院重症监护室患者标本共24例,对分离到的耐碳青霉烯类肺炎克雷伯菌进行全自动细菌生化鉴定药敏分析,并通过脉冲场凝胶电泳DNA指纹图谱对菌株进行比对,确定菌株同源性,同时结合临床资料进行分析。结果 该研究耐碳青霉烯类肺炎克雷伯菌患者下呼吸道感染占58.3%,仅极少数感染者(小于30%)对多黏菌素B敏感,对其余药物均耐药。该菌院内获得感染者23例占95.83%。24例耐碳青霉烯类肺炎克雷伯菌感染者死亡13例占54.2%。通过脉冲场凝胶电泳DNA指纹图谱对菌株进行比对发现,24例耐碳青霉烯类肺炎克雷伯菌菌株亲缘关系较强,仅极少数菌株间无亲缘关系或亲缘关系较差。结论 该院耐碳青霉烯类肺炎克雷伯菌患者主要感染部位为下呼吸道感染,药物敏感性极差,菌株同源性较强,院内感染极为严重,治疗困难,死亡率较高。
Abstract:
Objective To investigate the epidemiological investigation of carbapenem-resistant Klebsiella pneumoniae(CRKP)isolated from intensive care unit patients,and provide reference for clinical treatment and monitoring.Methods A total of 24 specimens from the intensive care unit of the Chinese Medicine Hospital of Pidu in Chengdu,from January 2018 to January 2019,the isolated CRKP was subjected to a automatic bacterial biochemical identification drug sensitivity analysis,and the strains were compared by pulsed gel electrophoresis DNA fingerprinting to determine the homology of the strain.At the same time,combined with clinical data for analysis.Results In this study,patients with CRKP accounted for 58.3% of lower respiratory tract infections,and patients with CRKP only had a resistance rate of less than 30% for polymyxin B,and 100% for the remaining drugs.One case of CRKP was infected with infected patients,accounting for 4.17%.23 cases of infected patients in the hospital accounted for 95.83%,and 24 patients with CRKP infection died(54.2%).The strains were aligned by pulse gel electrophoresis DNA fingerprinting.24 cases of CRKP strains had strong genetic relationship.Only a few strains were unrelated or had poor genetic relationship.Conclusion The main infection site of patients with CRKPinfection was lower respiratory tract infection in this hospital,drug sensitivity was very poor,nosocomial infection was serious,strain homology was strong,treatment was difficult,and mortality was extremely high.

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相似文献/References:

[1]刘 云,万玉香,马 炜,等.VITEK 2 Compact检测耐碳青霉烯类肺炎克雷伯菌对阿米卡星药敏结果的准确性评价[J].现代检验医学杂志,2018,33(01):133.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　LIU Yun,WAN Yu-xiang,MA Wei,et al.Evaluation on the Accuracy of VITEK 2 Compact for the Susceptibility of Carbapenem-Resistant Klebsiella Peumoniae to Amikacin[J].Journal of Modern Laboratory Medicine,2018,33(04):133.[doi:10.3969/j.issn.1671-7414.2018.01.001]

备注/Memo

备注/Memo:
作者简介:周朝琼(1995-),女,本科,技师,主要研究方向:临床微生物学检验及临床生物化学检验,E-mail:1340709790@qq.com。收稿日期:2019-03-22 修回日期:2019-04-06
更新日期/Last Update: 2019-07-30