[1]刘涵轩,伍 波,赵正春,等.布鲁氏菌血清 IgM, IgG 抗体胶体金免疫层析检测方法的建立及初步临床应用[J].现代检验医学杂志,2020,35(06):102-105.[doi:doi:10.3969/j.issn.1671-7414.2020.06.025]
 LIU Han-xuan,WU Bo,ZHAO Zheng-chun,et al.Preliminary Clinical Application of Colloidal Gold ImmunochromatographicDetection of Brucella IgM, IgG Antibodies[J].Journal of Modern Laboratory Medicine,2020,35(06):102-105.[doi:doi:10.3969/j.issn.1671-7414.2020.06.025]
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布鲁氏菌血清 IgM, IgG 抗体胶体金免疫层析检测方法的建立及初步临床应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年06期
页码:
102-105
栏目:
论著
出版日期:
2020-12-30

文章信息/Info

Title:
Preliminary Clinical Application of Colloidal Gold ImmunochromatographicDetection of Brucella IgM, IgG Antibodies
文章编号:
1671-7414(2020)06-102-05
作者:
刘涵轩1 伍 波1 赵正春1 陈 超1 韩 露1 梁 辰2 薛 辉2 冯 杰2 颜光涛2
(1. 北京中检安泰诊断科技有限公司,北京 102600;2. 解放军总医院第一医学中心医学检验中心,北京 100853)
Author(s):
LIU Han-xuan 1 WU Bo 1 ZHAO Zheng-chun 1 CHEN Chao 1 HAN Lu 1 LIANG Chen 2 XUE Hui 2 FENG Jie 2 YAN Guang-tao 2
(1.Beijing Zhongjian Antai Diagnostic Technology Co.Ltd, Beijing 102600,China;2.Center for Clinical LaboratoryMedicine, the First Medical Center, Chinese PLA General Hospital, Beijing 100853, China)
关键词:
胶体金布鲁氏菌快速检测性能验证
分类号:
R378.5;R446.61
DOI:
doi:10.3969/j.issn.1671-7414.2020.06.025
文献标志码:
A
摘要:
目的 应用胶体金免疫层析技术建立一种快速检测布鲁氏菌 IgM,IgG 抗体的方法,并进行初步的性能验证。方法 采用枸椽酸三钠还原法制备胶体金颗粒,标记纯化的抗重组布鲁氏菌抗原,将鼠抗人 IgG 抗体、鼠抗人 IgM 抗体和兔抗布鲁氏菌抗体喷在硝酸纤维素膜上作为检测线和质控线,对 pH 值和抗体浓度等条件优化;评估试剂盒灵敏度、特异度、交叉反应、准确度、精密度和干扰实验;用该方法对临床的 400 份血清进行检测,试管凝集试验验证其准确性。结果 该试纸条最佳胶体金标记的 pH 值为 7.5,标记重组抗原质量浓度为 30?g/ml。方法的灵敏度和稳定性较好且与其他病原菌无交叉反应。血清总胆红素、血脂和血红蛋白浓度的增高会使试纸条显色结果减弱。对400份临床样本进行检测,其结果与试管凝集试验的符合率为 93.75%。结论 该试验建立的布鲁氏菌 IgM/IgG 抗体的胶体金免疫层析检测方法具有较好的特异度和稳定性,整个检测过程可在 15min 内完成,能够快速检测样品血清,适用于现场快速检测。

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更新日期/Last Update: 2020-12-20