[1]张治洋,李功娟.10株宫颈癌细胞系中MALAT1的表达及其对顺铂敏感性的影响[J].现代检验医学杂志,2021,36(05):110-113.[doi:10.3969/j.issn.1671-7414.2021.05.024]
 ZHANG Zhi-yang,LI Gong-juan.Expression of MALAT1 in 10 Cervical Cancer Cell Lines and Its Effect onCisplatin Sensitivity[J].Journal of Modern Laboratory Medicine,2021,36(05):110-113.[doi:10.3969/j.issn.1671-7414.2021.05.024]
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10株宫颈癌细胞系中MALAT1的表达及其对顺铂敏感性的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年05期
页码:
110-113
栏目:
研究简报·实验技术
出版日期:
2021-10-14

文章信息/Info

Title:
Expression of MALAT1 in 10 Cervical Cancer Cell Lines and Its Effect onCisplatin Sensitivity
文章编号:
1671-7414(2021)05-110-05
作者:
张治洋1李功娟2
(1. 安康市人民医院妇科,陕西安康 725000 ;2. 安康市汉滨区第一医院妇科,陕西安康 725000)
Author(s):
ZHANG Zhi-yang1 LI Gong-juan2
(1. Department of Gynecology, Ankang People’s Hospital,Shaanxi Ankang 725000,China;2. Department ofGynecology, the First Hospital of Hanbin District, Shaanxi Ankang 725000,China)
关键词:
宫颈癌肺腺癌转录相关转录本1顺铂
分类号:
R737.33;R730.43
DOI:
10.3969/j.issn.1671-7414.2021.05.024
文献标志码:
A
摘要:
目的 探究肺腺癌转录相关转录本1(metastasis associated lung adenocarcinoma transcript 1, MALAT1)在10株宫颈癌(cervical cancer, CC)细胞中的表达及其对顺铂敏感性的影响。方法 选取10株CC细胞系(BT-B,C-33A,CaSKi,MEG-01,Hela,HELA-S3,Ishikawa,RL95-2,HCC94和MS751),通过CCK-8实验验证MALAT1对10株宫颈癌细胞增殖的影响;通过化疗敏感性实验验证MALAT1对10种宫颈癌细胞化疗敏感性的影响;通过荧光素酶报告实验检测MALAT1野生质粒的荧光素酶活性。结果 miR-126-5p在宫颈癌细胞株中的表达与MALAT1呈负相关(R=-0.673, P=0.033)。干扰MALAT1能够抑制宫颈癌细胞增殖(均P<0.05),干扰MALAT1能够增加宫颈癌细胞系对顺铂的敏感性(均P<0.05)。过表达miR-126-5p能够抑制宫颈癌细胞增殖(P<0.05),过表达miR-126-5p能够增加宫颈癌细胞系对顺铂的敏感性(P<0.05)。结论 MALAT1在宫颈癌中能够通过靶向调控miR-126-5p的表达,进而调节宫颈癌细胞对顺铂的敏感性,进而导致宫颈癌患者对顺铂产生耐药。
Abstract:
Objective To explore the expression and effect on the chemoresistance. Methods Detected the effect of metastasisassociated lung adenocarcinoma transcript 1 (MALAT1) on proliferation on 10 cervical cancer cells using CCK-8 assay,and deteced the effect of MALAT1 on sensitivity to chemotherapy on 10 cervical cancer cells using improved CCK-8 assay,Luciferase activity of MALAT1 wild plasmid was detected by luciferase reporter assay. Results The expression of miR-126-5pin cervical cancer cell line was negatively correlated with MALAT1 (r=-0.673, P=0.033). Knockdown MALAT1 could inhibitthe proliferation of cervical cancer cells (all P<0.05). Knockdown MALAT1 increased the sensitivity of cervical cancer celllines to cisplatin (all P<0.05). Overexpression of miR-126-5p could inhibit the proliferation of cervical cancer cells (P<0.05).Over-expression of miR-126-5p could increase the sensitivity of cervical cancer cell lines to cisplatin (P<0.05). Conclusion MALAT1 can regulate the expression of miR-126-5p in cervical cancer, thereby regulating the sensitivity of cervical cancer cellsto cisplatin, and thereby leading to cisplatin resistance in patients with cervical cancer.

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备注/Memo

备注/Memo:
作者简介:张治洋(1976-),女,本科,副主任医师,研究方向:宫颈癌、子宫内膜癌、卵巢癌,E-mail: shanzhangzhiyang@163.com。
通讯作者:李功娟(1986-),女,本科,主治医师,研究方向:滋养细胞肿瘤、妇科恶性肿瘤,E-mail:398658431@qq.com。
更新日期/Last Update: 1900-01-01