[1]杜龙庭,勿坡巫且,许春梅,等.缺血性脑卒中患者血清miR-155和PDCD4mRNA表达水平与颈动脉支架成形术后再狭窄的相关性研究[J].现代检验医学杂志,2022,37(02):105-109.[doi:10.3969/j.issn.1671-7414.2022.02.022]
　DU Long-ting,WUPO Wu-qie,XU Chun-mei,et al.Correlation between the Expression of miR-155, PDCD4 mRNA and Restenosis after Carotid Artery Stenting in Patients with Cerebral Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2022,37(02):105-109.[doi:10.3969/j.issn.1671-7414.2022.02.022]
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缺血性脑卒中患者血清miR-155和PDCD4mRNA表达水平与颈动脉支架成形术后再狭窄的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年02期
页码:
105-109
栏目:
论　著
出版日期:
2022-03-15

文章信息/Info

Title:
Correlation between the Expression of miR-155, PDCD4 mRNA and Restenosis after Carotid Artery Stenting in Patients with Cerebral Ischemic Stroke
文章编号:
1671-7414（2022）02-105-06
作者:
杜龙庭1; 勿坡巫且1; 许春梅1; 李　炜2
（1. 凉山彝族自治州第二人民医院神经内科，四川凉山 615000；2. 陆军特色医学中心神经内科，重庆 400042）
Author(s):
DU Long-ting1;  WUPO Wu-qie1;  XU Chun-mei 1;  LI Wei2
（1. Department of Neurology,the Second People’s Hospital of Liangshan Yi Autonomous Prefecture, Sichuan Liangshan 615000,China; 2. Department of Neurology,Army Characteristic Medical Center, Chongqing 400042, China）
关键词:
缺血性脑卒中; 微小RNA-155; 程序性细胞死亡因子4; 术后再狭窄
分类号:
R743.33；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.02.022
文献标志码:
A
摘要:
目的　了解缺血性脑卒中（cerebral ischemic stroke，CIS）患者血清微小RNA-155（microRNA-155，miR-155）、程序性细胞死亡因子4（programmed cell death factor 4，PDCD4）信使RNA（messenger RNA，mRNA）表达水平与颈动脉支架成形术（carotid artery stenting，CAS）后再狭窄的相关性。方法　选择2016 年2 月～ 2020 年7 月在凉山彝族自治州第二人民医院确诊的CIS 患者120 例作为CIS 组，同期选取健康体检者125 例为对照组。实时荧光定量PCR 法测定CIS 组术前和术后24 h，对照组体检时血清miR-155 和PDCD4 mRNA 表达水平；术后180 天对CIS 患者进行脑多普勒超声检查和颈动脉彩超检查，观察CIS 患者颈动脉狭窄程度、不稳定斑块数量、斑块长度及斑块厚度，测定患者血浆粘度、全血黏度、红细胞比容和纤维蛋白原水平；采用受试者工作特征（receiver operating characteristic，ROC）曲线分析血清miR-155 和PDCD4 mRNA 表达水平对CIS 患者行CAS 术后再狭窄的诊断价值。结果　与对照组相比，CIS 组术前血清miR-155 表达水平显著降低（0.31±0.06 vs 1.01±0.20）（q=45.189，P=0.000），CIS 组术前（2.23±0.45 vs 1.03±0.20）和术后24 h（1.19±0.29 vs 1.03±0.20）血清PDCD4 mRNA 表达水平均显著升高（q=40.422，5.390，均P=0.000），差异均有统计学意义；与CIS 组术前相比，CIS 组术后24 h 血清miR-155 表达水平显著升高（0.97±0.21 vs 0.31±0.06）（q=42.179，P=0.000），血清PDCD4 mRNA 表达水平显著降低（1.19±0.23 vs2.23±0.45）（q=34.680，P=0.000），差异均有统计学意义；与未狭窄组相比，再狭窄组颈动脉狭窄程度（21.31±5.33vs 9.11±2.52）、术后24 h 血清PDCD4 mRNA 表达水平（2.18±0.54 vs 1.06±0.26）、不稳定斑块数量（5.51±1.27 vs2.87±0.71）、斑块长度（7.60±1.90mm vs 3.16±0.79mm）、斑块厚度（4.60±1.15mm vs 2.84±0.71mm）、血浆黏度（15.03±3.85mPa·s vs 11.01±2.75mPa·s）、全血粘度(5.84±1.46mPa·s vs 3.71±0.92mPa·s）、红细胞比容（58.98%±17.74%vs 46.51%±11.62%）和纤维蛋白原（4.93±1.23g/L vs 3.01±0.75g/L）显著升高，差异均有统计学意义（t=4.277 ～ 17.874，均P<0.05），术后24 h 血清miR-155 表达水平显著降低（0.36±0.09 vs 1.03±0.25），差异有统计学意义（t=9.074，P=0.000）。ROC曲线结果表明，术后24 h血清miR-155和PDCD4 mRNA表达水平诊断CAS术后再狭窄的AUC分别为0.778（95% CI 为0.692~0.864）和0.838（95% CI 为0.749~0.926）。结论　血清miR-155 和PDCD4 mRNA 的异常表达与CIS病情及CAS 术后再狭窄联系密切，对CAS 术后再狭窄具有一定的诊断价值。
Abstract:
Objective　To detect the expression levels of microRNA-155 (miR-155) and programmed cell death factor 4 (PDCD4) messenger RNA (mRNA) in patients with cerebral ischemic stroke (CIS) and analyze the relationship between them and restenosis after carotid artery stenting (CAS) for CIS patients. Methods　A total of 120 cases of CIS patients diagnosed in the Second People’s Hospital of Liangshan Yi Autonomous Prefecture from February 2016 to July 2020 were selected as the CIS group, and 125 cases of healthy people were selected as the control group. The expression levels of miR-155 and PDCD4 mRNA in serum of CIS group were measured by real-time quantitative PCR before and 24 hours after operation, and in control group at physical examination. 180 days after operation, the patients with CIS were examined by cerebral Doppler ultrasound and carotid artery color Doppler ultrasound, the degree of carotid stenosis, the number of unstable plaques, the length and thickness of plaques were observed. The plasma viscosity, whole blood viscosity, hematocrit and fibrinogen levels were measured. Receiver operating characteristic(ROC) curve was used to analyze the diagnostic value of serum miR-155 and PDCD4 mRNA expression levels in the diagnosis of restenosis after CAS in patients with CIS. Results　Compared with those in the control group, the expression level of serum miR-155 of CIS group before operation was significantly lower(0.31±0.06 vs 1.01±0.20) (q=45.189, P=0.000),and the expression level of serum PDCD4 mRNA in CIS group before operation (2.23±0.45 vs 1.03±0.20) and 24 hours after operation (1.19±0.29 vs 1.03±0.20) was significantly higher (q=40.422, 5.390, all P=0.000)，the differences were statistically significant，respectively. Compared with the CIS group before operation, the expression level of serum miR-155 of CIS group at 24 h after operation was significantly increased (0.97±0.21 vs 0.31±0.06) (q=42.179, P=0.000), and the expression level of serum PDCD4 mRNA was significantly decreased (1.19±0.23 vs 2.23±0.45) (q=34.680, P=0.000), the differences were statistically significant, respectively. And compared with the non stenosis group, the degree of carotid artery stenosis(21.31%±5.33% vs 9.11%±2.52%), the expression level of serum PDCD4 mRNA 24 hours after operation(2.18±0.54 vs 1.06±0.26), the number of unstable plaques(5.51±1.27 vs 2.87±0.71), plaque length(7.60±1.90mm vs 3.16±0.79mm), plaque thickness(4.60±1.15mm vs 2.84±0.71mm), plasma viscosity(15.03±3.85mPa·s vs 11.01±2.75mPa·s), whole blood viscosity(5.84±1.46mPa·s vs 3.71±0.92mPa·s), hematocrit(58.98%±17.74% vs 46.51%±11.62%) and fibrinogen(4.93±1.23g/L vs 3.01±0.75g/L) in the restenosis group were significantly increased ，the differences were statistically significant (t=4.277~17.874, all P<0.05), and the expression level of serum miR-155 24 hours after operation was significantly decreased (0.36±0.09 vs 1.03±0.25), the difference was statistically significant (t=9.074, P=0.000). ROC curve results showed that the AUC of serum miR-155 and PDCD4 mRNA expression levels 24 hours after operation in the detection of restenosis after CAS was 0.778 (95% CI: 0.692~0.864) and 0.838 (95% CI: 0.749~0.926) respectively. Conclusion　The abnormal expression of serum miR-155 and PDCD4 mRNA was closely related to the condition of CIS and restenosis after CAS, which has certain diagnostic value for restenosis after CAS.

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备注/Memo

备注/Memo:
基金项目：四川省医学科研青年创新课题（Q18010）。
作者简介：杜龙庭（1985-），男，本科，主治医师，研究方向：脑血管疾病诊治，E-mail：dulongting582@sina.com。
通讯作者：李炜（1991-），男，硕士，主治医师，研究方向：脑血管介入，E-mail：294251048@qq.com。
更新日期/Last Update: 1900-01-01