[1]周仕群,王　聪,江　强,等.糖尿病肾病患者血清miR-188-5p及miR-379-5p的表达及其临床意义[J].现代检验医学杂志,2022,37(03):91-95.[doi:10.3969/j.issn.1671-7414.2022.03.019]
　ZHOU Shi-qun,WANG Cong,JIANG Qiang,et al.Expression and Clinical Significance of Serum miR-188-5p and miR-379-5p in Patients with Diabetic Nephropathy[J].Journal of Modern Laboratory Medicine,2022,37(03):91-95.[doi:10.3969/j.issn.1671-7414.2022.03.019]
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糖尿病肾病患者血清miR-188-5p及miR-379-5p的表达及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
91-95
栏目:
论　著
出版日期:
2022-05-15

文章信息/Info

Title:
Expression and Clinical Significance of Serum miR-188-5p and miR-379-5p in Patients with Diabetic Nephropathy
文章编号:
1671-7414（2022）03-091-05
作者:
周仕群; 王　聪; 江　强; 陈岳尧; 符妹丽
（儋州市人民医院肾内科，海南儋州 571179）
Author(s):
ZHOU Shi-qun;  WANG Cong;  JIANG Qiang;  CHEN Yue-yao;  FU Mei-li
（Department of Nephrology，Danzhou People’s Hospital，Hainan Danzhou 571799，China）
关键词:
2 型糖尿病; 糖尿病肾病; 微小核糖核酸-188-5p; 微小核糖核酸-379-5p
分类号:
R587.2；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.03.019
文献标志码:
A
摘要:
目的　探讨糖尿病肾病（ diabetic nephropathy，DN）患者血清微小核糖核酸（ micro RNA, miR）-188-5p及微小核糖核酸（micro RNA, miR）-379-5p的表达及其临床意义。方法　选取 2018年 1月～ 2020年 12月儋州市人民医院收治的 2型糖尿病患者 162例和健康体检正常者 50例（对照组）。根据尿清蛋白 /尿肌酐 (urinary albumin /creatinine， UACR)比值，将 162例 2型糖尿病患者分为 54例 2型糖尿病组（ UACR<30mg/g）和 108例 DN组（UACR ≥ 30mg/g）。 108例 DN患者分为微量清蛋白尿组 50例（UACR 30～ 300mg/g）和大量清蛋白尿组 58例（UACR＞ 300mg/g）。比较各组血清 miR-188-5p及 miR-379-5p水平，应用多因素 Logistic回归分析影响 DN发生的危险因素。绘制受试者工作特征（ ROC）曲线分析 miR-188-5p，miR-379-5p及尿微量清蛋白（ Microalbumin，mALB）水平诊断 DN的价值。结果　 DN组血清 miR-188-5p（0.20±0.08）及 miR-379-5p（0.57±0.16）水平均明显低于 2型糖尿病组（ 0.71±0.20，1.15±0.35）和对照组（ 1.06±0.35，1.84±0.71），差异均有统计学意义（ t=10.316～ 16.218，均 P＜ 0.05）。大量清蛋白尿组血清 miR-188-5p及 miR-379-5p水平均明显低于微量清蛋白尿组，差异均有统计学意义（ t=10.926，12.714，均 P＜ 0.05）。多因素 Logistic回归分析显示， miR-188-5p，miR-379-5p及 mALB是影响 2型糖尿病患者发生 DN的独立危险因素（ P＜ 0.05）。ROC曲线显示，miR-188-5p，miR-379-5p及 mALB三项联合诊断 DN的曲线下面积（0.952，95%CI：0.890～ 0.997）最大，其敏感度和特异度分别为 97.4%和 85.3%。结论　DN患者血清 miR-188-5p，miR-379-5p水平明显降低，是 DN发生的独立危险因素，联合 mALB在 DN诊断中具有较好的价值。
Abstract:
Objective　To investigate the expression and clinical significance of serum miR-188-5p and miR-379-5p in patients with diabetic nephropathy (DN). Methods　From January 2018 to December 2020, 162 patients with type 2 diabetes and 50 healthy subjects (control group) in Danzhou People’s Hospital were selected. According to the ratio of urinary albumin to urinary creatinine (UACR), 162 patients with type 2 diabetes were divided into 54 patients with type 2 diabetes mellitus（UACR<30mg/g） and 108 patients with DN (UACR ≥ 30 mg/g). The 108 patients with DN were divided into 50 cases in the microalbuminuria group (UACR 30 ～ 300mg/g) and 58 cases in the massive albuminuria group (UACR ＞ 300mg/g). The levels of serum miR- 188-5p and miR-379-5p in each group were compared, and the risk factors affecting the occurrence of DN were analyzed by multivariate Logistic regression analysis. Receiver operating characteristic (ROC) curve was drawn to analyze the diagnostic value of miR-188-5p，miR-379-5p and mALB level in patients with DN. Results　The serum miR-188-5p（0.20±0.08） and miR-379-5p （0.57±0.16）levels in DN group were significantly lower than those in type 2 diabetes group（0.71±0.20， 1.15±0.35）and control group（1.06±0.35，1.84±0.71）, the differences were statistically significant (t=10.316 ～ 16.218， all P ＜ 0.05). The levels of serum miR-188-5p and miR-379-5p in macroalbuminuria group were significantly lower than those in microalbuminuria group, and the differences were statistically significant (t=10.926, 12.714, all P < 0.05). Multivariate logistic regression analysis showed that miR-188-5p, miR-379-5p and mAlb were independent risk factors for DN in patients with type 2 diabetes mellitus (P < 0.05). ROC curve showed that the area under the curve (0.938, 95%CI: 0.877 ～ 0.993) of miR-188-5p, miR-379-5p and mAlb in the joint diagnosis of DN was the largest, and their sensitivity and specificity were 97.4% and 85.3%， respectively. Conclusion　The levels of serum miR-188-5p and miR-379-5p were significantly lower in patients with DN, which were independent risk factors for the occurrence of DN, and combined with mAlb has a good value in the diagnosis of DN.

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备注/Memo

备注/Memo:
作者简介：周仕群（1981-）, 女，主治医师，本科，主要从事肾内疾病诊治研究，E-mail：shiqunzhou1127@163.com。
更新日期/Last Update: 1900-01-01