[1]夏爱军,樊文昕,黄珊珊,等.孕妇血清高效价IgG 血型抗体亚型分析与胎儿新生儿溶血病及高胆红素血症发生率的相关性研究[J].现代检验医学杂志,2023,38(02):181-185.[doi:10.3969/j.issn.1671-7414.2023.02.034 ]
 XIAai-jun,FAN Wen-xin,HUANG Shan-shan,et al.Analysis of High Titer IgG Blood Group Antibody Subtype in Pregnant Women’s Serum and Its Correlation with the Incidence of Hemolytic Disease and Hyperbilirubinemia of the Fetus and Newborn[J].Journal of Modern Laboratory Medicine,2023,38(02):181-185.[doi:10.3969/j.issn.1671-7414.2023.02.034 ]
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孕妇血清高效价IgG 血型抗体亚型分析与胎儿新生儿溶血病及高胆红素血症发生率的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年02期
页码:
181-185
栏目:
检验与临床
出版日期:
2023-03-15

文章信息/Info

Title:
Analysis of High Titer IgG Blood Group Antibody Subtype in Pregnant Women’s Serum and Its Correlation with the Incidence of Hemolytic Disease and Hyperbilirubinemia of the Fetus and Newborn
文章编号:
1671-7414(2023)02-181-05
作者:
夏爱军1樊文昕2黄珊珊1郝 茜1赵雅琦1
(1.西安大兴医院输血科,西安 710016;2.西安市人民医院 /西安市第四医院皮肤激光治疗中心,西安 710005)
Author(s):
XIAai-jun1 FAN Wen-xin2 HUANG Shan-shan1HAO Xi1 ZHAO Ya-qi1
(1.Department of Blood Transfusion, Xi’an Daxing Hospital,Xi’an 710016, China; 2.Department of Dermatology, Xi’an People’s Hospital / the Fourth Hospital of Xi’an, Xi’an 710005,China)
关键词:
孕妇血型抗体IgG亚型胎儿新生儿溶血病高胆红素血症
分类号:
R722.18;R457.11
DOI:
10.3969/j.issn.1671-7414.2023.02.034
文献标志码:
A
摘要:
目的 研究高效价 IgG血型抗体孕妇血清中 IgG亚型与胎儿新生儿溶血病(hemolytic disease of the fetus and newborn, HDFN)及高胆红素血症发生率的相关性,评价 IgG亚型检测的临床价值。方法 对 2020年 1月~2021年 10月在西安大兴医院进行产前免疫血清学检测的 225例高效价 IgG抗体孕妇(其中 ABO血型系统 217例,Rh血型系统 8例),采用微柱凝胶技术检测其 IgG亚型,对新生儿脐血进行新生儿溶血系列检测,生化分析仪检测新生儿血清胆红素水平 ,分析孕妇血清 IgG亚型与 HDFN及高胆红素血症发生率的相关性。结果 217例高效价 IgG抗 -A(B)孕妇血清中 IgG1/IgG3亚型 134例(占比 61.75%), IgG1/IgG3混合型 18例(占比 8.29%),IgG2/IgG4亚型检出 65例(占比 29.95%);8例高效价 Rh系统 IgG抗体均为 IgG1和 /或 IgG3亚型,未检出 IgG2或 IgG4亚型。 IgG1/IgG3亚型组中 HDFN发生率为 79.29%(111/140),高胆红素血症发生率为 55.71%(78/140);IgG1/IgG3混合型组中 HDFN发生率为 90.00%(18/20),高胆红素血症发生率为 70.00%(14/20);IgG2/IgG4亚型组中 HDFN发生率为 15.38%(10/65),未发生高胆红素血症。 IgG1/IgG3亚型组与 IgG1/IgG3混合型组 HDFN及高胆红素血症发生率比较差异无统计学意义(χ2=0.692,1.461,均 P> 0.05);IgG抗体效价 1 ∶ 256及效价 1 ∶ 512,HDFN发生率分别为 64.28%,56.94%, IgG1/IgG3单独存在组、混合型组和 IgG2/IgG4组随抗体效价增高 HDFN发生率未增加,差异均无统计学意义(χ2=0.231, 2.813,均 P>0.05)。结论 血型抗体高效价孕妇血清中 IgG亚型类别与 HDFN及高胆红素血症发生率具有相关性,产前检测血型抗体 IgG亚型,对 HDFN及高胆红素血症的精准预防和治疗具有指导意义。
Abstract:
Objective To study the correlation between high titer IgG blood group antibody subtypes in pregnant women and postpartum hemolytic disease and hyperbilirubinemia of the newborn(HDFN),in order to evaluate the clinical value of IgG subtype detection.Methods Serum IgG subtypes were detected by microcolum gel technology assay in 225 pregnant women with high titer antibody [217 of ABO blood group system and 8 of Rh system] before delivery from January 2020 to October 2021 in Xi’an Daxing Hospital. Neonatal hemolysis tests and serum bilirubin levels in the umbilical cord blood of the new born were detected. The relationship between serum IgG subtype of pregnant women and HDFN and the incidence of hyperbilirubinemia were analyzed. Results There were 134 cases of IgG1/IgG3 subtype (61.75%), 18 cases of IgG1/IgG3 mixed subtype (8.29%) and 65 cases of IgG2/IgG4 subtype (29.95%) in the serum of 217 pregnant women with high titer ABO system IgG anti-A (B). The 8 cases of high titer Rh system IgG antibodies were all of IgG1 and/or IgG3 subtype, no IgG2 or IgG4 subtype was detected. In the IgG1/IgG3 subtype group, the incidence of HDFN was 79.29%(111/140), and the incidence of hyperbilirubinemia was 55.71%(78/140). In the mixed IgG1/IgG3 group, the incidence of HDFN was 90%(18/20), and the incidence of hyperbilirubinemia was 70%(14/20). In the IgG2/IgG4 subtype group, the incidence of HDFN was 15.38%(10/65), and no hyperbilirubinemia occurred. There were no significant differences in the incidence of HDFN and hyperbilirubinemia between IgG1/IgG3 subtype group and IgG1/IgG3 mixed type group (χ2=0.692, 1.461, all P>0.05). The incidence of HDFN for IgG anti-A(B) titer 1∶256 and 1∶512 groups were 64.28% and 56.94%, respectively. The incidence of HDFN did not increase with higher antibody titer in the IgG1/IgG3, IgG1/IgG3 mixed and IgG2/IgG4 subtype groups (χ2=0.231,2.813, all P>0.05). Conclusion The serum IgG subtype of pregnant women was closely related to the incidence of HDFN and hyperbilirubinemia. It is necessary to carry out prenatal detection of blood group antibody IgG subtype, which is of great significance for guiding accurate prevention and effective treatment in clinic.

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备注/Memo

备注/Memo:
收稿日期:2022-01-09修回日期:2022-11-09
作者简介:李涤蓉(1975-),女,大专,主管技师,研究方向:临床免疫, E-mail:di_lirong@163.com。
通讯作者:赵蓉(1988-),女,硕士,主管技师,研究方向:临床免疫, E-mail:13711281497@163.com。

更新日期/Last Update: 2023-03-15