[1]张 咪,杨春利,成 军,等.应用海藻糖脂核酸免提取技术对宫颈分泌物前处理进行HPV核酸检测的实验评价[J].现代检验医学杂志,2025,40(02):191-194.[doi:10.3969/j.issn.1671-7414.2025.02.036]
　ZHANG Mi,YANG Chunli,CHENG Jun,et al.Application Evaluation of Nucleic Acid Detection of HPV in Cervical Secretions Based on Algal Glycolipid Nucleic Acid-free Extraction Technology[J].Journal of Modern Laboratory Medicine,2025,40(02):191-194.[doi:10.3969/j.issn.1671-7414.2025.02.036]
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应用海藻糖脂核酸免提取技术对宫颈分泌物前处理进行HPV核酸检测的实验评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
191-194
栏目:
研究简报·实验技术
出版日期:
2025-03-15

文章信息/Info

Title:
Application Evaluation of Nucleic Acid Detection of HPV in Cervical Secretions Based on Algal Glycolipid Nucleic Acid-free Extraction Technology
文章编号:
1671-7414（2025）02-191-04
作者:
张 咪; 杨春利; 成 军; 戴玉柱
（中国人民解放军联勤保障部队第九〇三医院检验科，杭州 310013）
Author(s):
ZHANG Mi;  YANG Chunli;  CHENG Jun;  DAI Yuzhu
（Department of Clinical Laboratory, the 903rd Hospital of the People’s Liberation Army, Hangzhou 310013, China）
关键词:
人乳头瘤病毒; 前处理; 海藻糖脂核酸免提取技术
分类号:
R446；R373
DOI:
10.3969/j.issn.1671-7414.2025.02.036
文献标志码:
A
摘要:
目的 采用海藻糖脂核酸免提取技术在宫颈分泌物人乳头瘤病毒（HPV）基因分型检测前处理，评价该技术在HPV 检测中的应用价值。方法 收集2023 年7 月1 日～ 9 月30 日联勤保障部队第九〇三医院50 例HPV 检测样本，并选取12 份HPV 核酸检测质控品（不同亚型），采用海藻糖脂免提取技术前处理作为实验组，临床常规前处理作为对照组，对该免提取技术用于检测HPV 的准确度、灵敏度和精密度进行评价，并分析两组方法的相关性。结果 实验组中临床样本（27 例阳性样本和23 例阴性样本）和质控品HPV 的分型结果与对照组一致，两组符合率100%，准确度良好；实验组检出限达到了100 copies/ml，灵敏度较高；精密度评价结果显示，高中低三个水平的临床样本和质控品的检测CV 均＜ 5%，精密度良好；两组检测结果（Ct 值）的相关性评价显示，回归方程为Y=2.524+0.901X，r=0.963，实验组与对照组间HPV 核酸检测Ct 值具有良好的相关性。结论 海藻糖脂核酸免提取技术可用于临床宫颈分泌物HPV 核酸检测前处理，适合临床应用推广。
Abstract:
Objective To evaluate the application value of algal glycolipid nucleic acid-free extraction technology in human papillomaviruses (HPV) nucleic acid of cervical secretions. Methods From July 1 to September 30, 2023, 50 HPV samples were collected from the 903rd Hospital of PLA, and 12 quality control products of HPV nucleic acid were selected. Samples treated with algal glycolipid nucleic acid-free extraction technology were tested as the experimental group, samples treated with clinical routine detection kit were tested as the control group. The nucleic acid-free extraction technology for HPV detection was assessed for accuracy, sensitivity, precision and the correlation between the two methods. Results The HPV typing results of clinical samples (27 positive samples and 23 negative samples) and quality control products in the experimental group were consistent with those in the control group, with a 100% coincidence rate and high accuracy. The experimental group’s detection limit reached 100 copies/ml, demonstrating great sensitivity. The intra-assay coefficient of variations were less than 5%, indicating good precision. The detection results (Ct values) of the two groups showed a strong correlation (P<0.05), and the linear regression equation was Y=2.524+0.901X, r=0.963. There was a good correlation between the Ct values of HPV nucleic acid testing between the experimental group and the control group. Conclusion Algal glycolipid nucleic acid-free extraction technology can be used in clinical cervical secretion HPV nucleic acid detection before treatment, which is suitable for clinical application and promotion.

参考文献/References:

[1] OYOUNI A A A. Human papillomavirus in cancer: infection, disease transmission, and progress in vaccines[J]. Journal of Infection and Public Health, 2023, 16(4): 626-631.
[2] WILLIAMSON A L. Recent developments in Human papillomavirus (HPV) vaccinology[J]. Viruses, 2023, 15(7): 1440.
[3] GAMBOA-HOIL S I. Human papillomavirus in men[J]. Revista Internacional de Andrologia, 2023, 21(1): 100325.
[4] NGO-METZGER Q, ADSUL P. Screening for cervical cancer[J]. American Family Physician, 2019, 99(4): 253-254.
[5] 沈华, 盛晓鹃. HPV 阳性宫颈癌组织中IGF2BP2 和RPRD1B 的表达水平及临床价值研究[J]. 现代检验医学杂志, 2023, 38(5): 121-126. SHEN Hua, SHENG Xiaojuan. Expression and clinical value of IGF2BP2 and RPRD1B in HPV positive cervical cancer tissues[J]. Journal of Modern Laboratory Medicine, 2023, 38(5): 121-126.
[6] BRUNI L, SERRANO B, ROURA E, et al. Cervical cancer screening programmes and age-specific coverage estimates for 202 countries and territories worldwide: a review and synthetic analysis[J]. the Lancet Global Health, 2022, 10(8): e1115-e1127.
[7] YANG Yueming, XU Lijiang, YUAN Songhua, et al. Optimal screening and detection strategies for cervical lesions: a retrospective study[J]. Journal of Cancer, 2024, 15(11): 3612-3624.
[8] DERBIE A, AMARE B, MISGAN E, et al. Histopathological profile of cervical punch biopsies and risk factors associated with high-grade cervical precancerous lesions and cancer in northwest Ethiopia[J]. PLoS One, 2022, 17(9): e0274466.
[9] 田美月, 王源, 陈迎弟, 等. HR-HPV 持续感染患者阴道微生态与阴道灌洗液CCL28 水平表达的相关性研究[J]. 现代检验医学杂志, 2024, 39(4): 155-160. TIAN Meiyue, WANG Yuan, CHEN Yingdi, et al. Study on the correlation between the vaginal microecology and CCL28 levels expression in the vaginal lavage fluid of patients with persistent HR-HPV infection[J]. Journal of Modern Laboratory Medicine, 2024, 39(4): 155-160.
[10] VO D T, STORY M D. Facile and direct detection of human papillomavirus (HPV) DNA in cells using loop-mediated isothermal amplification (LAMP)[J].Molecular and Cellular Probes, 2021, 59: 101760.
[11] 中国人民解放军联勤保障部队第九〇三医院. 一种通用型鼻咽拭子核酸免提取试剂及其使用方法?应用: 中国, CN116426608A [P/OL]. 2023-03-07 (2023-07-14). http://static.tian yancha.com/patent/content PDF/ooace936ef769c14fcaf33757d4dd6c8.pdf. The 903rd Hospital of People’s Liberation Army. The invention relates to a general nasopharyngeal swab nucleic acid free extraction reagent, a method for use and application there of: China, CN116426608A [P/OL]. 2023-03-07 (2023-07-14). http://static.tian yancha.com/patent/content PDF/ooac e936ef769c14fcaf33757d4dd6c8.pdf.
[12] 惠海英, 宫艳艳, 弥鹏. 西安地区女性尖锐湿疣患者人乳头瘤病毒分型及与复发关系分析[J]. 现代检验医学杂志, 2017, 32(4): 140-142. HUI Haiying, GONG Yanyan, MI Peng. Study on relationship of human papillomavirus genotyping and recrudescence for female patients with condyloma acuminatum in Xi’an[J]. Journal of Modern Laboratory Medicine, 2017, 32(4): 140-142.
[13] SHEN Ye, HUANG Yanru, WANG Wenbo, et al. Prevalence and genotype distribution of HPV infection among women in Xiamen, China[J]. Frontiers in Microbiology, 2023, 14: 1130226.
[14] MARKOWITZ L E, SCHILLER J T. Human papillomavirus vaccines[J]. the Journal of Infectious Diseases, 2021, 224(12 Suppl 2): S367-S378.
[15] WANG Renjie, PAN Wei, JIN Lei, et al. Human papillomavirus vaccine against cervical cancer: opportunity and challenge[J]. Cancer Letters, 2020, 471: 88-102.
[16] LI Mingzhu, ZHAO Chao, ZHAO Yun, et al. Immunogenicity, efficacy, and safety of human papillomavirus vaccine: data from China[J]. Frontiers in Immunology, 2023, 14: 1112750.
[17] 黄媛媛, 王宇, 周承星, 等. 呼吸道病毒核酸快速检测方案的建立与应用[J]. 生物工程学报, 2023,39(9): 3838-3848. HUANG Yuanyuan, WANG Yu, ZHOU Chengxing, et al. Development and application of a rapid scheme for detection of respiratory virus nucleic acid[J]. Chinese Journal of Biotechnology, 2023, 39(9): 3838-3848.
[18] 刘丽. 乙型肝炎病毒核酸免提取荧光定量PCR 方法应用价值分析[J]. 中国医疗器械信息, 2021, 27(16): 60, 137. LIU Li. Application value of quantitative PCR method for non-extraction of hepatitis B virus nucleic acid[J]. China Medical Device Information, 2021, 27(16): 60, 137.
[19] 郭鹏波, 章波, 王彦红, 等. 免提取冻干式百日咳鲍特菌核酸检测方法建立及应用[J]. 实验与检验医学,2021, 39(4): 789-791, 801. GUO Pengbo, ZHANG Bo, WANG Yanhong, et al. Establishment of freeze-dried and direct amplification Q-PCR method for B. pertussis[J]. Experimental and Laboratory Medicine, 2021, 39(4): 789-791, 801.

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备注/Memo

备注/Memo:
基金项目：浙江省自然科学基金（批准号：LGF20H200009）；第九〇三医院自主科研项目（课题编号：ZJ202301）。
作者简介：张咪（1995-），女，学士，初级检验技师，研究方向：分子生物学，E-mail：597617938@qq.com。
通讯作者： 戴玉柱（1985-），男，硕士，硕士生导师，副主任技师，研究方向：临床感染性疾病的分子诊断，E-mail：dyz5895@qq.com。
成军（1967-），男，硕士，硕士生导师，主任医师，研究方向：病原诊断学，E-mail：cj1171967@163.com。
更新日期/Last Update: 2025-03-15