[1]陈晓露.白细胞介素4基因多态性与结直肠癌的易感性[J].现代检验医学杂志,2015,30(05):105-106.[doi:10.3969/j.issn.1671-7414.2015.05.032]
　CHEN Xiao-lu.Interleukin-4 Gene Polymorphisms and Colorectal Cancer Susceptibility[J].Journal of Modern Laboratory Medicine,2015,30(05):105-106.[doi:10.3969/j.issn.1671-7414.2015.05.032]
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白细胞介素4基因多态性与结直肠癌的易感性()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
105-106
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Interleukin-4 Gene Polymorphisms and Colorectal Cancer Susceptibility
作者:
陈晓露
西安市中心医院消化科，西安710003
Author(s):
CHEN Xiao-lu
Department of Gastroenterology，Xi’an Central Hospital，Xi’an 710003，China
关键词:
白细胞介素4; 基因多态性; 结直肠肿瘤
分类号:
R730.43；R735.3
DOI:
10.3969/j.issn.1671-7414.2015.05.032
文献标志码:
A
摘要:
目的研究白细胞介素-4 (interleukin-4，IL-4)基因多态性与结直肠癌的相关性。方法采用聚合酶链式反应-序列特异性引物(PCR-SSP)方法及聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测93例结直肠癌患者及107例健康对照者的IL-4内含子3及启动子-589位点基因型。结果结直肠癌组IL-4内含子3的RP1基因频率明显低于对照组(69.9% vs 86.4%，χ2＝16.300，P<0.01)，RP2基因频率明显高于对照组(30.1% vs 13.6%，χ2＝16.300，P<0.01)。RP1.1，RP1.2基因型的优势比值分别为0.28(95%CI，0.15～0.50)和3.28(95%CI，1.77～6.07)。IL-4-589位点在结直肠癌组和对照组中基因型分布和等位基因频率与内含子3位点频率分布一致，连锁不平衡。结论结直肠癌与IL-4基因多态性相关联。
Abstract:
ObjectiveTo investigate the association between the interleukin-4(IL-4) gene polymorphisms and colorectal cancer．MethodsGenotypes of the IL-4 intron 3 and -589 loci of the IL-4 promoter were examined by the polymerase chain reaction with sequence-specific primers (PCR-SSP) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) respectively in 93 colorectal cancer patients and 107 healthy controls．ResultsThe gene frequency of allele RP1 of interleukin-4 intron 3 in colorectal cancer patients was significantly lower than that in healthy controls (69.9% vs 86.4%，χ2＝16.300，P<0.01)，but the gene frequency of RP2 was significantly higher than that in healthy controls (30.1% vs 13.6%，χ2＝16.300，P<0.01)．OR of the genotype RP1.1 and RP1.2 were 0.28 (95%CI，0.15～0.50) and 3.28 (95%CI，1.77～6.07) respectively．The distributing frequencies of genotypes and alleles of IL-4-589 locus in healthy controls and in colorectal cancer patients were consistent with those of IL-4 intron 3 locus．There was a linkage disequilibrium between 2 locus．ConclusionThere is correlation between the colorectal cancer and the gene polymorphisms of IL-4．

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备注/Memo

备注/Memo:
作者简介：陈晓露(1980-)，女，医学硕士，主治医师，消化内科专业，Tel：029-87268355-3066，E-mail：ccwhite@163.com。
更新日期/Last Update: 1900-01-01