[1]刘冲,唐浩,邓霖,等.血清miRNA-186,miRNA-30c在肝细胞性癌中诊断价值的研究[J].现代检验医学杂志,2016,31(06):44-47.[doi:10.3969/j.issn.1671-7414.2016.06.012]
　LIU Chong,TANG Hao,DENG Lin,et al.Research of Diagnostic Application of Serum miRNA-186 and miRNA-30c in Patients with Hepatocellular Carcinoma[J].Journal of Modern Laboratory Medicine,2016,31(06):44-47.[doi:10.3969/j.issn.1671-7414.2016.06.012]
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血清miRNA-186,miRNA-30c在肝细胞性癌中诊断价值的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
44-47
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Research of Diagnostic Application of Serum miRNA-186 and miRNA-30c in Patients with Hepatocellular Carcinoma
作者:
刘冲; 唐浩; 邓霖; 张静; 邓建平
黄石市爱康医院有限责任公司检验科,湖北黄石 435000
Author(s):
LIU Chong; TANG Hao; DENG Lin; ZHANG Jing; DENG Jian-ping
Department of Clinical Laboratory, Huangshi City Love-Health Hospital Co.LTD,Hubei Huangshi 435000,China
关键词:
肝癌;  miR-186;  miR-30c;  甲胎蛋白;  诊断
分类号:
R735.3; R730.43
DOI:
10.3969/j.issn.1671-7414.2016.06.012
文献标志码:
A
摘要:
目的 分析miRNA-186,miRNA-30c在肝细胞性肝癌(hepatocellular carcinoma,HCC)诊断方面潜在的应用价值。方法 收集肝细胞性肝癌、慢性肝炎及健康体检者各55例; 以逆转录PCR方法检测miRNA-186,miRNA-30c在血清中的相对表达情况,以受试者工作曲线分析两者在肝癌诊断中的应用价值并与血清甲胎蛋白(alpha-fetoprotein,AFP)检测相比较。结果 肝癌患者血清中的miRNA-186相对表达量高于慢性肝炎及健康对照组(P<0.01,P=0.02),miRNA-30c的相对表达量低于慢性肝炎及健康对照组(P=0.034,P<0.01),血清miRNA-186,miRNA-30c ROC曲线下面积分别为0.927和0.932,miRNA-186诊断肝癌的灵敏度和特异度分别为78.18%和63.64%,miRNA-30c诊断肝癌的灵敏度和特异度分别为81.82%,71.82%,分别以miR-186+miR-30c,miR-186+AFP,miR-30c+AFP和miR-186+miR-30c+AFP作为联合诊断组合时,诊断灵敏度分别为89.09%,85.45%,90.9%和94.54%。特异度分别为66.36%,67.27%,82.73%和53.64%。结论 miR-30c与AFP联合检测时在显著提升诊断敏感度的同时,特异度仍维持在较好水平,且准确度也相对最高,是较为理想的肝癌诊断组合。
Abstract:
Objective To explore the diagnostic value of miRNA-186 and miRNA-30c in patients with hepatocellular carcinoma(HCC).Methods Gene expression of miRNA-186 and miRNA-30c were examined in 55 serum samples from hepatocellular carcinoma(HCC),liver cirrhosis(LC)and health volunteers by quantitative real-time reverse transcription polymerasechain reaction(RT-qPCR).The diagnostic efficiency of miRNA-186 and miRNA-30con HCC were analyzed by receiver operating characteristiccurve(ROC)and compared with alpha-fetoprotein(AFP).Results Expression levels of miR-186 in HCC sera were significant higher than those in other groups(P<0.01 and P=0.02)and miR-30c in HCC sera were significant lower thanthose in other groups(P=0.034 and P<0.01).The areas under ROC of miRNA-186 and miRNA-30c in sera were 0.927 and 0.932 respectively.miRNA-186 had a sensitivity of 78.18% and a specificity of 63.64% and miR-30c had a sensitivity of 81.82% and a specificity of 71.82% in discriminating HCC from CHB,respectively.The HCC diagnostic sensitivity was up to 89.09%,85.45%,90.9% and 94.54% with the specificity of 66.36%,67.27%,82.73% and 53.64% in the combination of miR-186+miR-30c,miR-186+AFP,miR-30c+AFP and miR-186+miR-30c+AFPrespectively.Conclusion Combination of miR-30c and AFPimproved the sensitivity while maintain a relative high specificity,so this combination is worth to be used in clinical practice.

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备注/Memo

备注/Memo:
基金项目:湖北省黄石市科技攻关项目,项目编号:HWJ2016-11-057。 作者简介:刘 冲(1987-),男,硕士,从事分子诊断研究,E-mail:LC19870715@sina.com。 通讯作者:邓建平(1967-),男,硕士,从事细胞生物学研究,E-mail:djp226255@163.com。
更新日期/Last Update: 2016-12-20