[1]彭　琦a,程少鹏b,陈辅萍a.非小细胞肺癌组织中NSE 的基因表达及与患者临床特征的相关性研究[J].现代检验医学杂志,2020,35(03):36-38.[doi:10.3969/j.issn.1671-7414.2020.03.008]
　PENG Qia,CHENG Shao-pengb,CHEN Fu-pinga.Correlation between NSE Gene Expression and Serum NSE Level in Tumor Tissues and Clinical Characteristics of Patients with Non-Small Cell Lung Cancer[J].Journal of Modern Laboratory Medicine,2020,35(03):36-38.[doi:10.3969/j.issn.1671-7414.2020.03.008]
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非小细胞肺癌组织中NSE 的基因表达及与患者临床特征的相关性研究 ()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
36-38
栏目:
论著
出版日期:
2020-07-20

文章信息/Info

Title:
Correlation between NSE Gene Expression and Serum NSE Level in Tumor Tissues and Clinical Characteristics of Patients with Non-Small Cell Lung Cancer
文章编号:
1671-7414（2020）03-036-03
作者:
彭　琦a; 程少鹏b; 陈辅萍a
（商洛市中心医院a. 呼吸内科；b. 检验中心，陕西商洛　726000）
Author(s):
PENG Qia;  CHENG Shao-pengb;  CHEN Fu-pinga
（a. Department of Respiratory; b. Department of Clinical Laboratory,Shangluo Central Hospital, Shaanxi Shangluo 726000, China）
关键词:
非小细胞肺癌; 神经元特异性烯醇化酶; 临床特征
分类号:
R734.2；R730.43
DOI:
10.3969/j.issn.1671-7414.2020.03.008
摘要:
目的　探讨非小细胞肺癌(non-small cell lung cancer, NSCLC) 患者肿瘤组织中神经元特异性烯醇化酶 (neuron-specific enolase, NSE) 的基因表达及与患者临床特征的相关性。方法　选取2013 年1 月～ 2017 年12 月商 洛市中心医院157 例NSCLC 患者为研究对象，采用电化学发光法检测患者血清中NSE 的水平，采用PCR 法比较 NSCLC 组织和相应癌旁正常组织中NSE 的相对表达量，并且探究NSE 的表达水平与患者临床特征间( 年龄、性别、 是否吸烟、是否饮酒、临床分期、是否原位癌、有无淋巴结和远端转移) 的相关性。结果　NSE 在NSCLC 组织中的 相对表达量显著高于相应癌旁正常组织，其差异具有统计学意义 (P=0.0182)。NSE 表达水平与NSCLC 患者是否吸烟 (χ2 ＝ 9.959，P=0.002)、是否饮酒(χ2 ＝ 4.893，P=0.032)、临床分期(χ2 ＝ 11.070，P=0.001)、是否原位癌(P<0.001)、 有无淋巴结(P<0.001) 和远端转移显著相关(P=0.017)。结论　NSE 在NSCLC 组织中高表达，血清NSE 水平可作为 NSCLC 诊断和预后的生物指标。
Abstract:
Objective　To investigate the correlation between neuron-specific enolase (NSE) gene expression and serum NSE level in tumor tissues of non-small cell lung cancer (NSCLC) patients and the clinical characteristics of patients. Methods　A total of 157 NSCLC patients collected in Shangluo Central Hospital from January 2013 to December 2017 were selected as the study subjects. The serum level of NSE was tested by the electrochemical luminescence method and the PCR method was adopted to compare relative expression of NSE in NSCLC tissues and adjacent normal tissue, and the correlation of NSE levels and the clinical features of patients (age, sex, smoking, drinking, clinical stage, carcinoma in situ, lymph nodes and distal metastasis) was explored. Results　The relative expression level of NSE in NSCLC tissues was significantly higher than that in adjacent normal tissues, and the difference was statistically significant (P=0.0182). NSE expression level was significantly correlated with smoking (χ2 ＝ 9.959，P=0.002), drinking (χ2 ＝ 4.893，P=0.032), clinical stage (χ2 ＝ 11.070，P=0.001), cancer in situ (P<0.001), presence of lymph nodes (P<0.001), and distal metastasis (P=0.017) in NSCLC patients. Conclusion　NSE was highly expressed in NSCLC tissues and serum NSE level can be used as a biomarker for the diagnosis and prognosis of NSCLC.

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备注/Memo

备注/Memo:
收稿日期：2020-01-08　修回日期：2020-03-13 作者简介：彭琦(1974- )，男，本科，副主任医师，研究方向：呼吸肿瘤、慢性肺阻，E-mail: shanglpengqi@163.com
更新日期/Last Update: 2020-07-20