[1]沈　玲,白　欢,李　丽,等.AUTOF MS 1000 质谱鉴定系统对临床实验室常见菌株鉴定能力的评估[J].现代检验医学杂志,2020,35(03):100-102.[doi:10.3969/j.issn.1671-7414.2020.03.026]
　SHEN Ling,BAI Huan,LI Li,et al.Evaluation of Identification Ability of Common Strain in Clinical Laboratory with AUTOF MS 1000 Mass Spectrometry Identification System[J].Journal of Modern Laboratory Medicine,2020,35(03):100-102.[doi:10.3969/j.issn.1671-7414.2020.03.026]
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AUTOF MS 1000 质谱鉴定系统对临床实验室常见菌株鉴定能力的评估()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
100-102
栏目:
研究简报·实验技术
出版日期:
2020-07-20

文章信息/Info

Title:
Evaluation of Identification Ability of Common Strain in Clinical Laboratory with AUTOF MS 1000 Mass Spectrometry Identification System
文章编号:
1671-7414（2020）03-100-03
作者:
沈　玲;  白　欢;  李　丽;  汪　玥;  龚　路;  孙自镛;  陈中举
（华中科技大学同济医学院附属同济医院检验科，武汉 430030）
Author(s):
SHEN Ling ;  BAI Huan ;  LI Li ; WANG Yue ;  GONG Lu ;  SUN Zi-yong ; CHEN Zhong-ju
（Department of Laboratory Medicine , Tongji Hospital , Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430030, China）
关键词:
Autof MS 1000; 质谱鉴定系统; 菌株鉴定
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2020.03.026
摘要:
目的　评估Autof MS 1000 质谱鉴定系统对临床实验室常见菌株的鉴定能力。方法　参考CLSI M52 标准对 Autof MS 1000 质谱鉴定系统进行评估。菌株鉴定的评估指标包括准确度和精密度。准确度评估以Bruker MALDI-TOF 作为参比设备，以Autof MS 1000 为待评估设备，选取231 株能够涵盖实验室80% ～ 90% 的常见分离菌株及1 株室间 质评菌株作为检测标本，包括革兰氏阳性需氧菌77 株，革兰氏阴性需氧菌95 株，厌氧菌30 株，酵母样真菌30 株，待 评估设备鉴定结果与参比设备鉴定结果一致时判断为相符，若结果不一致则以测序方法（细菌为16S RNA，真菌为ITS 区测序）鉴定结果为准。精密度评估为选取12 株室内质控菌株和临床分离菌株作为检测标本，在Autof MS 1000 质谱 鉴定系统上进行重复性测试，每株菌重复检测3 次，判断其鉴定结果的一致性。以准确度大于90％、精密度大于95％ 为判断合格标准。结果　232 株临床实验室常见分离菌株鉴定结果准确度为100%；12 株室内质控菌株和临床分离菌株 重复性检测结果的精密度为100%。结论　Autof MS 1000 质谱鉴定系统对临床实验室常见菌株鉴定的准确度和精密度 都很高，并且具有快速、低耗及易操作等优势，建议可在临床微生物实验室推广使用。
Abstract:
Objective　To evaluate the identification ability of Autof MS1000 for the common strains in clinical laboratory. Methods　Autof MS 1000 was evaluated followed by the reference of the CLSI M52 standard, chosing the utmost evaluation indexes of accuracy and precision. The accuracy was assessed between Autof MS1000 and Bruker MALDI-TOF, which was regarded as a reference instrument , by selecting 231 fresh clinical strains and 1external quality assessment(EQA) strain, including 77 Gram-positive aerobic strains, 95 Gram-negative aerobic strains, 30 anaerobic strains and 30 yeast-like fungi strains, which could cover 80% to 90% of the common strains in clinical laboratory. When there was a discrepancy between above two , a third method ,ribosomal 16s RNA sequencing was used to confirm the results. The precision was assessed by testing the repeatability on Autof MS1000 for three times each, by choosing 12 EQA strains,which included 4 Gram-positive aerobic strains, 6 Gram - negative aerobic strains and 2 yeast fungi strains. Results　The conformity of the identification results of 232 common strains isolated in clinical laboratory was 100%, and the precision of the repeatability of 12 EQA strains each was 100%. Conclusion　The accuracy was 100%, which was higher than 90% of CLSI M52 required, and also the precision is 100%, which was greater than 95% of that required. Futhermore, Autof MS1000 has the advantages of quickly and accurately identifing the strains with low cost and simple operation.It is suggested that it should be popularized in clinical laboratory.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2019-12-31　修回日期：2020-02-07基金项目：湖北省自然科学基金面上项目（2019CFB666）。 作者简介：沈玲（1990-），女，大学本科，技师，主要研究方向为临床微生物学检验和感染性疾病的诊断，E-mail：baobeisling@163.com。 通讯作者：陈中举，E-mail：hailong1228@163.com
更新日期/Last Update: 2020-07-20