[1]林晓晖,麦惠香,简梦华,等.梅里埃VTIEK MS 质谱仪在甲氧西林耐药及敏感的金黄色葡萄球菌快速鉴定的应用研究[J].现代检验医学杂志,2020,35(05):121-123.[doi:10.3969/j.issn.1671-7414.2020.05.031]
　LIN Xiao-hui,MAI Hui-xiang,JIAN Meng-hua,et al.Study on VTIEK MS Mass Spectrometer Rapid Identification of Methicillin Resistance and Sensitive Staphylococcus Aureus[J].Journal of Modern Laboratory Medicine,2020,35(05):121-123.[doi:10.3969/j.issn.1671-7414.2020.05.031]
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梅里埃VTIEK MS 质谱仪在甲氧西林耐药及敏感的金黄色葡萄球菌快速鉴定的应用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
121-123
栏目:
研究简报·实验技术
出版日期:
2020-11-01

文章信息/Info

Title:
Study on VTIEK MS Mass Spectrometer Rapid Identification of Methicillin Resistance and Sensitive Staphylococcus Aureus
文章编号:
1671-7414（2020）05-121-03
作者:
林晓晖; 麦惠香; 简梦华; 朱剑霞; 邱伟波
广州市番禺区中心医院，广州 511400
Author(s):
LIN Xiao-hui;  MAI Hui-xiang;  JIAN Meng-hua;  ZHU Jian-xia; QIU Wei-bo
Panyu Central Hospital of Guangzhou,Guangzhou 511400,China
关键词:
耐甲氧西林金黄色葡萄球菌;  基质辅助激光解吸/ 电离飞行时间质谱仪;  甲氧西林敏感金黄色葡萄 球菌
分类号:
R378.11；R446.5
DOI:
10.3969/j.issn.1671-7414.2020.05.031
文献标志码:
A
摘要:
目的　探讨梅里埃VTIEK MS 质谱仪快速鉴定甲氧西林耐药金黄色葡萄球菌（MRSA）及甲氧西林敏感的金 黄色葡萄球菌(MSSA) 的可行性。方法　对广州市番禺区中心医院2018 ～ 2019 年各个病区住院患者在不同部位分离的 30 株MRSA 及20 株MSSA，用VITEK 2 Compact 的药敏卡AST-P639 进行耐药性检测，用VTIEK MS 对所有菌株进 行鉴定。结果　用VITEK MS 的Launch pad 软件对30 株MRSA 及20 株MSSA 所产生的质谱峰图m/z 进行分析，质谱 峰m/z 的 3 009，5 624 和6 887 为金黄色葡萄球菌的特征峰，质谱峰m/z 2 637 及4 308 为区分MRSA 及MSSA 最主要 的特征峰，其中质谱峰m/z 2 637 MSSA 组较MRSA 组低，质谱峰m/z 4 308 则MRSA 组较MSSA 组高，不同菌株质谱 峰略有不同。结论　梅里埃VTIEK MS 可快速准确鉴别MRSA 及MSSA，且具有耗时短、灵敏度高、特异性好等特点。
Abstract:
Objective　To explore the feasibility of VTIEK MS mass spectrometer rapid identification of methicillin resistance aureus（MRSA）and sensitive Staphylococcus aureus(MSSA). Methods　30 MRSA and 20 MSSA was isolated by the patients in various disease areas of Guangzhou Panyu Center Hospital from 2018 to 2019,that with VITEK 2 Compact’s drug-sensitive card AST-P639 for drug resistance testing, and VITEK MS to identify the strain. Results　Analysis of the mass spectrometry peak m/z produced by VITEK MS certified MRSA and MSSA using VITEK MS Launch pad software, mass spectrometry peak m/z of 3 009, 5 624, 6 887 was the characteristic peak of Staphylococcus aureus, mass spectrometry peak m/z 2 637 and 4 308 were the most important characteristic peaks to distinguish MRSA from MSSA, where mass spectrometry peak m/z 2 637 MSSA group was lower than the MRSA group, the mass spectrometry peak m/z 4 308 was higher than MSSA, and the mass spectrometry peak skewed of different strains was slightly different. Conclusion　VTIEK MS can quickly and accurately identify MRSA and MSSA, which are characterized by short time-consuming, high sensitivity and good specificity.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：广州市卫计局资助项目（20171A010335）。
作者简介：林晓晖（1976-），女，硕士，主任技师，主要从事微生物检验，E-mail: carollinxh@163.com。
更新日期/Last Update: 2020-10-30