[1]李晓刚a,邓巧妮a,王军焕a,等.儿童哮喘患者外周血 ILC2 细胞和 Th2 型细胞相关因子水平变化及与临床相关性研究[J].现代检验医学杂志,2020,35(06):72-77.[doi:doi:10.3969/j.issn.1671-7414.2020.06.018]
 LI Xiao-gang a,DENG Qiao-ni a,WANG Jun-huan a,et al.Changes of ILC2 Cells and Th2 Cell Related Factors in Peripheral Blood ofChildren with Asthma and Their Clinical Relevance[J].Journal of Modern Laboratory Medicine,2020,35(06):72-77.[doi:doi:10.3969/j.issn.1671-7414.2020.06.018]
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儿童哮喘患者外周血 ILC2 细胞和 Th2 型细胞相关因子水平变化及与临床相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年06期
页码:
72-77
栏目:
论著
出版日期:
2020-12-30

文章信息/Info

Title:
Changes of ILC2 Cells and Th2 Cell Related Factors in Peripheral Blood ofChildren with Asthma and Their Clinical Relevance
文章编号:
1671-7414(2020)06-072-06
作者:
李晓刚1a 邓巧妮1a 王军焕1a 葛君俐1b 康 茹2
(1. 宝鸡高新人民医院 a. 儿科;b. 检验科,陕西宝鸡 721000;2. 西北妇女儿童医院检验科,西安 710061)
Author(s):
LI Xiao-gang 1a DENG Qiao-ni 1a WANG Jun-huan 1a GE Jun-li 1b KANG Ru 2
(1a. Department of Pediatrics; 1b. Department of Clinical Laboratory, Baoji Gaoxin People’s Hospital, Shaanxi Baoji721000,China;2.Department of Clinical Laboratory,Northwest Women’s and Children’s Hospital,Xi’an 710061,China)
关键词:
儿童支气管哮喘 2 型固有免疫细胞Th2 型细胞因子
分类号:
R725.6;R392.11
DOI:
doi:10.3969/j.issn.1671-7414.2020.06.018
文献标志码:
A
摘要:
目的 探讨支气管哮喘患儿外周血中 2 型固有免疫细胞(type2 innate lymphoid cells ILC2)和 Th2 型细胞相关因子水平变化及其临床意义。方法 选取 2019 年 1 月 ~2020 年 5 月在宝鸡高新人民医院确诊的 67 例急性发作期支气管哮喘患儿作为研究对象,根据发病严重程度分为重度 - 危重组 23 例和轻 - 中度组 44 例;并选取同期健康体检的 45例儿童作为对照组。检测两组血清 ILC2 细胞比例和 IL-4,IL-5,IL-10 和 IL-13 水平,记录两组肺功能指标:第一秒用力呼气量(FEV1)、用力肺活量(FVC)及最大呼气峰流速(PEF);并分析 ILC2 细胞比例和 IL-4,IL-5,IL-10 和IL-13 水平与肺功能指标的相关性。结果 支气管哮喘组患儿外周血 ILC2 细胞比例及 IL-4,IL-5 和 IL-13 水平明显高于对照组,差异均有统计学意义(F=24.419~209.627,均 P<0.001);IL-10 水平及肺功能指标 FVC,FEV1 和 PEF 明显低于对照组,差异均有统计学意义(F=80.826~298.942,均 P<0.001)。重度 - 危重组支气管哮喘患儿外周血 ILC2 细胞比例和 IL-4,IL-5,IL-10 和 IL-13 水平及肺功能指标变化较轻 - 中度组更为显著,差异均有统计学意义(t=1.990~7.848,P 均 <0.05)。支气管哮喘患儿外周血 ILC2 细胞比例及 IL-4,IL-5 和 IL-13 水平与 FVC,FEV1 和 PEF 值呈显著负相关,差异均有统计学意义(r=0.564~0.712,均 P<0.01);IL-10 水平与 FVC,FEV1 和 PEF 值呈显著正相关,差异均有统计学意义(r=0.537~0.603,均 P<0.01)。结论 急性发作期支气管哮喘患儿外周血 ILC2 细胞比例及 Th2 型细胞相关因子水平明显升高或降低,与哮喘患儿肺功能及病情严重程度显著相关,对其进行检测有助于评估支气管哮喘患儿病情程度,为临床提供理论指导。

参考文献/References:

[1] 李昌崇 , 朱丽丽 . 儿童支气管哮喘急性发作期治疗进展:各国支气管哮喘防治指南比较 [J]. 中华实用儿科临床杂志 ,2017,32(16):1209-1214.LI Changchong,ZHU Lili. Progress in the treatmentof childhood bronchial asthma exacer bations:com-parison of guidelines for bronchical asthma in differentcountries[J]. Chinese Journal of Applied Clinical Pedi-atrics, 2017, 32(16): 1209-1214.
[2] GRAYSON M H,FELDMAN S,PRINCE B T, et al.2017 年哮喘研究进展 : 发病机制、生物制剂和遗传学 [J]. 杨玲,刘婷,译 . 中华临床免疫和变态反应杂志 ,2018,12(6):75-87.GRAYSON M H,FELDMAN S,PRINCE B T, et al.Progress in asthma research in 2017: pathogenesis,biologics and genetics [J]. YANG Ling,LIU Ting,Translation.Chinese Journal of Allergy & ClinicalImmunology, 2018,12(6):673-685.
[3] CAMINATI M, PHAM D L, BAGNASCO D, et al.Type 2 immunity in asthma [J]. The World AllergyOrganization Journal, 2018, 11(1): 13.
[4] 高阳,杨帆.支气管哮喘患者外周血 Th1, Th2 与Th17细胞表达水平及临床意义[J].解放军医药杂志,2017, 29(1):76-79.GAO Yang,YANG Fan. Expression levels and clinicalsignificances of Th1, Th2 and Th17 in peripheralblood of patients with bronchial asthma[J]. Medical &Pharmaceutical Journal of Chinese People’s LiberationArmy, 2017, 29(1): 76-79.
[5] 渠源,王硕,范祖森.ILC 细胞的生物学功能与免疫调节作用 [J]. 生物化学与生物物理进展,2018,45(9):897-914.QU Yuan, WANG Shuo, FAN Zusen. Research progresson the biological functions and immunoregulatoryeffects of innate lymphoid cells[J]. Progress inBiochemistry and Biophysics, 2018, 45(9): 897-914
[6] 刁渔汉 , 李艳 , 杨义然 , 等 .Th2 与 ILC2 细胞在哮喘模型中的数量及产生细胞因子的比较研究 [J].微生物学免疫学进展 ,2019,47(1):26-35.DIAO Yuhan, LI Yan, YANG Yiran, et al. Comparisonof quantity and cytokine production of Th2 and ILC2cells in murine models for asthma[J]. Progress in Mi-crobiology and Immunology,2019,47(1):26-35.
[7] 蒋芹,张建华.ILC2 参与哮喘发病机制的研究进展[J].临床儿科杂志,2017,35(2):148-152.JIANG Qin,ZHANG Jianhua. Progress on roles oftype 2 innate lymphoid cells in the pathogenesis ofasthma[J]. Journal of Clinical Pediatrics,2017,35 (2) :148-152.
[8] 杨丹 , 刘碧翠 , 刘春涛 . Ⅱ型固有淋巴细胞及其在支气管哮喘中的作用 [J]. 中华临床免疫和变态反应杂志 ,2018,12(1):74-80.YANG Dan,LIU Bicui,LIU Chuntao. Type Ⅱ in-nate lymphoid cells and its role in the pathogenesis ofasthma [J]. Chinese Journal of Allergy & Clinical Im-munology,2018,12(1):74-80.
[9] 中华医学会儿科学分会呼吸学组,《中华儿科杂志》编辑委员会 . 儿童支气管哮喘诊断与防治指南 (2016年版 )[J]. 中华儿科杂志 ,2016,54(3):167-181.The Subspecialty Group of Respiratory Diseases, TheSociety of Pediatrics, Chinese Medical Association ,Chinese Journal of Pediatrics Editorial Board.Guidelines for the diagnosis and prevention ofbronchial asthma in children (2016 edition)[J]. ChineseJournal of Pediatrics,2016,54(3):167-181.
[10] 魏勇 . 小儿支气管哮喘细胞免疫失衡的研究进展 [J].中国妇幼保健 ,2019,34(14):3375-3378.WEI Yong. Research progress of cellular immuneimbalance in children with bronchial asthma [J].Maternal & Child Health Care of China ,2019,34(14):3375-3378.
[11] 苗青,王燕,任亦欣,等.支气管哮喘患儿外周血不同 T 淋巴细胞亚群的表达及其临床意义 [J]. 中国妇幼保健 ,2017,32(18):4438-4441.MIAO Qing,WANG Yan,REN Yixin,et al.Expressions of different T-cell subsets in peripheralblood of children with bronchial asthma and the clinicalsignificance[J]. Maternal & Child Health Care of China,2017,32(18):4438-4441.
[12] MEUNIER I, MAROIS I, RICHTER M, et al.SAT0002 innate lymphoid cells(ILCS) are differentiallydistributed in inflammatory and non-inflammatory jointdiseases[J]. Annals of the Rheumatic Diseases, 2017,76(2): 769.
[13] SMITH S G, CHEN Ruchong, KJARSGAARD M,et al. Increased numbers of activated group 2 innatelymphoid cells in the airways of patients with severeasthma and persistent airway eosinophilia[J]. Journal ofAllergy and Clinical Immunology, 2016, 137(1): 75.
[14] GALLE-TREGER L, SUZUKI Y, PATEL N,etal.Nicotinic acetylcholine receptor agonist attenuatesILC2-dependent airway hyperreactivity[J]. NatureCommunications, 2016, 7:13202.
[15] 王忠平,胡翠萍,陆鸣,等.血清 IL-35, IL-17 表达水平及 25- 羟维生素 D3 水平与哮喘相互关系的临床研究[J].中国妇幼保健,2020,35(12):2231-2234.WANG Zhongping,HU Cuiping,LU Ming,et al.Clinical study on the relationship between serum IL-35and IL-17 expression levels and 25-hydroxyvitamin D3levels and asthma [J]. Maternal & Child Health Care ofChina,2020,35(12):2231-2234.
[16] 曾智敏,谭卫平,黄鑫炎,等.2 型固有免疫细胞在过敏性及非过敏性哮喘中的临床意义 [J]. 中山大学学报 ( 医学版 ),2018,39(4):532-539.ZENG Zhimin,TAN Weiping,HUANG Xinyan,et al. Clinical significance of type 2 innate lymphoidcells in allergic and non-allergic asthma[J].Journal ofSun Yat-sen University(Medical Sciences),2018,39(4):532-539.
[17] 张鑫 , 王炜 , 孙英 . 上皮源性细胞因子 IL-33, IL-25和 TSLP 在哮喘发病机制中的作用 [J]. 微生物学免疫学进展 ,2017,45(5):53-58.ZHANG Xin,WANG Wei,SUN Ying. Roles of epi-thelial-derived cytokines IL-33, IL-25 and TSLP in thepathogenesis of asthma[J]. Progress in Microbiologyand Immunology,2017,45(5):53-58.
[18] LEE E, LEE S H, KIM Y H, et al. Association of atopyphenotypes with new development of asthma and bron-chial hyperresponsiveness in school-aged children[J].Annals of Allergy, Asthma & Immunology, 2017,118(5): 542-550, e1.
[19] 范广来,徐妍,马希贵,等.哮喘患儿血清 IFN-γ,IL-4, IL-10 水平及与肺功能的关系 [J]. 中国现代医学杂志 , 2020, 30(7):48-52.FAN Guanglai,XU Yan,MA Xigui, et al. Significance of IFN-γ, IL-4 and IL-10 in serum of children withasthma[J]. China Journal of Modern Medicine, 2020,30(7):48-52.
[20] 曹华,屠强 . 支气管哮喘患者血清 IL-4,IL-10,IL-17 和 IFN-γ 水平测定及其临床意义 [J]. 国际检验医学杂志 ,2018,39(12):1506-1508.CAO Hua,TU Qiang. Determination of serum IL-4,IL-10, IL-17 and IFN- levels in patients with bronchialasthma and its clinical significance [J]. InternationalJournal of Laboratory Medicine,2018,39(12):1506-1508.
[21] 战海涛 , 范志强 , 陈新卫 , 等 . 血清 IL-6,IL-10 及TNF-α 的水平变化在支气管哮喘患者中的临床意义 [J]. 中国实验诊断学 ,2019,23(10):1741-1743.ZHAN Haitao,FAN Zhiqiang,CHEN Xinwei,etal. Clinical significance of serum IL-6, IL-10 and TNF-levels in patients with bronchial asthma [J]. ChineseJournal of Laboratory Diagnosis,2019,23(10):1741-1743.
[22] 王丹丹,柴若楠,戚菲菲 , 等 . Ⅱ型固有淋巴细胞在支气管哮喘发病机制中作用的研究进展 [J]. 中华微生物学和免疫学杂志 ,2016,36(8):634-638.WANG Dandan,CHAI Ruonan,QI Feifei,et al.Roles of type 2 innate lymphoid cells in the pathogene-sis of bronchial asthma[J]. Chinese Journal of Microbi-ology and Immunology,2016,36(08):634-638.
[23] 郭旭雪,聂汉祥,陈千慧,等.固有免疫细胞在支气管哮喘发病中的作用[J].细胞与分子免疫学杂志,2018,34(9):850-855.GUO Xuxue, NIE Hanxiang, CHEN Qianhui, et al.Role of innate immune cells in the pathogenesis ofbronchial asthma [J]. Chinese Journal of Cellular andMolecular Immunology, 2018, 34(9):850-855.

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更新日期/Last Update: 2020-12-20