[1]朱益佳,徐龙珍,胡 伟,等.两种试剂检测抗心磷脂抗体的比较[J].现代检验医学杂志,2015,30(02):94-96.[doi:10.3969/j.issn.1671-7414.2015.02.029]
　ZHU Yi-jia,XU Long-zhen,HU Wei,et al.Comparison of Two Kits in Determining Anti-Cardiolipin[J].Journal of Modern Laboratory Medicine,2015,30(02):94-96.[doi:10.3969/j.issn.1671-7414.2015.02.029]
点击复制

两种试剂检测抗心磷脂抗体的比较()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
94-96
栏目:
研究简报·实验技术
出版日期:
2015-03-20

文章信息/Info

Title:
Comparison of Two Kits in Determining Anti-Cardiolipin
作者:
朱益佳; 徐龙珍; 胡 伟; 陶 月; 张 葵; 王庆飞
南京大学医学院附属鼓楼医院检验科,南京 210008
Author(s):
ZHU Yi-jia; XU Long-zhen; HU Wei; TAO Yue; ZHANG Kui; WANG Qing-fei
Department of Clinical Laboratory,Nanjing Drum Tower Hospital, School of Medicine,Nanjing University,Nanjing 210008,China
关键词:
抗心磷脂抗体;  抗β2糖蛋白I抗体;  相关性
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2015.02.029
文献标志码:
A
摘要:
目的 对两种商品化的抗心磷脂抗体(Anti-cardiolipin,ACA)试剂的检测结果进行比较。方法 采用欧蒙公司ACA-IgA/G/M(总ACA)检测试剂和亚辉龙公司ACA-IgG,IgM和抗β2糖蛋白I(β2GPI)抗体IgG(ACA 3项)测定试剂盒,同时检测66例血清样本,比较两者结果的差异,并分析两者之间的关系。结果 欧蒙总ACA试剂的检出阳性率为37.88%(25/66); 亚辉龙ACA 3项试剂的检出阳性率为31.82%(21/66,ACA-IgG,IgM和抗β2GPI IgG三者任一阳性),两种试剂检测结果不具有统计学差异; 两者的检测符合率为87.88%(58/66)。欧蒙试剂ACA-IgA/G/M的检测结果与亚辉龙试剂ACA-IgG,IgM和抗β2GPI IgG 3个检测值的总和具有良好的相关性(r2=0.892,P<0.01)。结论 两种试剂检测结果符合性较高,具有良好的相关性,能满足临床应用的需要; 两种试剂各具特色,可以根据需要选择合适的试剂单独或联合进行检测。
Abstract:
Objective To compare the results of anti-cardiolipin(ACA)measuring by two commercial available kits.Methods ACA in total of 66 serum samples were both determined by kits from Euroimmun and YHLO simultaneously,then the results were analyzed comparatively and correlatively.The Euroimmun kit was applied to determination the level of ACA-IgA/G/M,and the YHLO kit determined ACA-IgG/M and anti-β2-glycoprotein I antibody(β2GPI IgG).Results The positive rate by Euroimmun kit was 37.88%(25/66),while 31.82%(21/66)was positive(one positive among ACA-IgG,IgM and β2GPI IgG)when determined by YHLO kit,and there was no significant difference between the two kits.The accordance rate of the two kits was 87.88%(58/66).The ACA-IgA/G/M value by Euroimmun kit and the summation ofACA-IgG,IgM and β2GPI IgG by YHLO kit showed well linear correlation(r2=0.892,P<0.01).Conclusion Results from the two kits were consistent and correlated well,and they are suitable for the clinical application; these two kits have their own characteristics,which could be used by individual or combination accordingly.

参考文献/References:

[1] Devreese KM.Antiphospholipid antibodies:evaluation of the thrombotic risk[J].Thromb Res,2012,130(Suppl 1):37-40.
[2] 刘 淳,刘彦虹.抗磷脂抗体促进血栓形成机制的研究[J].现代检验医学杂志,2014,29(1):16-19. Liu C,Liu YH.Research on antiphospholipid antibodies in the thrombosis and its pathogenesis[J].Journal of Modern Laboratory Medicine,2014,29(1):16-19.
[3] 张蜀澜,李永哲.抗磷脂抗体检测的现状及展望[J].中华检验医学杂志,2014,37(8):564-566. Zhang SL,Li YZ.Current status and prospects of antiphospholipid antibodies test[J].Chin J Lab Med,2014,37(8):564-566.
[4] Meroni PL,Chighizola CB,Rovelli F,et al.Antiphospholipid syndrome in2014:more clinical manifestations,novel pathogenic players and emerging biomarkers[J].Arthritis Res Ther,2014,16(2):209.
[5] Marai I,Gilbund B,Blank M,et al.Anti-cardiolipin and anti-β2-glycoprotein I(β2GPI)antibody assays as screening for anti-phospholipid syndrome[J].Hum Antibodies,2003,12(3):57-62.
[6] Yehudai D,Toubi E,Shoenfeld Y,et al.Autoimmunity and novel therapiesin immune-mediated thrombocytopenia[J].Semin Hematol,2013,50(Suppl 1):S100-S108.
[7] Levy RA,de Meis E,Pierangeli S.An adapted ELISA method for differentiating pathogenic from nonpathogenic aPL by a beta 2 glycoprotein I dependency anticardiolipin assay[J].Thromb Res,2004,114(5/6):573-577.
[8] Willis R,Grossi C,Orietta Borghi M,et al.International standards forIgG and IgM anti-β 2glycoprotein antibody measurement[J].Lupus,2014,23(12):1317-1319.
[9] Locht H,Wiik A.IgG and IgM isotypes of anti-cardiolipin and anti-beta2-glycoprotein I antibodies reflect different forms of recent thrombo-embolicevents[J].Clin Rheumatol,2006,25(2):246-250.
[10] 张蜀澜,吴子燕,陈 思,等.不同亚型抗磷脂抗体在系统性红斑狼疮诊断中的价值[J].中华检验医学杂志,2014,37(8):597-602. Zhang SL,Wu ZY,Chen S,et al.Clinical significance of different antiphospholipid antibody isotypes in systemic lupus erythematosus[J].Chin J Lab Med,2014,37(8):597-602.

相似文献/References:

[1]黄 杰,王 青,李良军,等.女性不孕症患者抗心磷脂抗体和抗β2-糖蛋白1抗体的检测及临床应用[J].现代检验医学杂志,2016,31(05):142.[doi:10.3969/j.issn.1671-7414.2016.05.042]
　HUANG Jie,WANG Qing,LI Liang-jun,et al.Detection and Clinic Application of Antiphospholipid Antibody and Anti-β2-GPⅠ in Female Infertility[J].Journal of Modern Laboratory Medicine,2016,31(02):142.[doi:10.3969/j.issn.1671-7414.2016.05.042]

备注/Memo

备注/Memo:
基金项目:南京市医学科技发展专项资金(YKK13066)。 作者简介:朱益佳(1990-),女,学士,技师,主要从事临床免疫学检验工作。 通讯作者:王庆飞(1983-),男,博士,Tel:025-83106666-60645,E-mail:wangqingfei@gmail.com。
更新日期/Last Update: 2015-03-20