[1]刘爱胜,文 艳.0.9 g/dl生理盐水稀释解决EDTA依赖性血小板假性减少的方法研究[J].现代检验医学杂志,2015,30(02):128-131.[doi:10.3969/j.issn.1671-7414.2015.02.040]
　LIU Ai-sheng,WEN Yan.Study on the Method of 0.9 g/dl Normal Saline Dilution to Solve EDTA Dependent Pseudothrombocytopenia[J].Journal of Modern Laboratory Medicine,2015,30(02):128-131.[doi:10.3969/j.issn.1671-7414.2015.02.040]
点击复制

0.9 g/dl生理盐水稀释解决EDTA依赖性血小板假性减少的方法研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
128-131
栏目:
研究简报·实验技术
出版日期:
2015-03-20

文章信息/Info

Title:
Study on the Method of 0.9 g/dl Normal Saline Dilution to Solve EDTA Dependent Pseudothrombocytopenia
作者:
刘爱胜1; 文 艳2
1.深圳市龙华新区人民医院检验科,广东深圳 518109; 2.深圳市光明新区人民医院ICU,广东深圳 518106
Author(s):
LIU Ai-sheng1; WEN Yan2
1.Department of Clinical Laboratory,Shenzhen Longhua New District People's Hospital,Guangdong Shenzhen 518109,China; 2.ICU,Shenzhen Guangming New District People's Hospital,Guangdong Shenzhen 518106,China
关键词:
0.9 g/dl生理盐水;  稀释仪器法;  乙二胺四乙酸二钾依赖性血小板假性减少症
分类号:
R446.11
DOI:
10.3969/j.issn.1671-7414.2015.02.040
文献标志码:
A
摘要:
目的 探讨0.9 g/dl生理盐水稀释仪器法解决乙二胺四乙酸二钾(EDTA-K2)抗凝剂依赖性血小板假性减少症(PTCP)的可行性,为临床实验室提供解决PTCP检测更为有效的方法。方法 对2014年8～10月深圳市龙华新区人民医院检验科确诊为PTCP案例共3例,采集静脉血2 ml 二份分别于EDTA-K2和枸椽酸钠抗凝管内混匀,于即刻,10,30,40及60 min上机检测; 同时分别采集末梢血于血细胞稀释液、0.9 g/dl生理盐水内混匀,于即刻,10,30,40及60 min上机检测,并与草酸铵手工法比较。结果 EDTA法、枸椽酸钠法、血细胞稀释液法及0.9 g/dl生理盐水稀释仪器法即刻检测PTCP血中血小板(PLT)结果与草酸铵法比较,差异均无统计学意义(t=0.943～1.537,P>0.05),10～60 min内EDTA-K2抗凝血PLT结果明显降低,与草酸铵法比较差异有统计学意义(t=12.413～49.162,P<0.01～0.001); 枸椽酸钠法于30 min后PLT开始下降,与草酸铵法比较,结果差异有统计学意义(t=4.915～20.164,P<0.05～0.01); 血细胞稀释液法于30～40 min PLT检测结果开始出现下降,但不明显,与草酸铵法比较差异无统计学意义(t=1.315～1.715,P>0.05),40～60 min PLT检测结果出现明显下降,与草酸铵法比较差异有统计学意义(t=3.175～3.865,P<0.05); 0.9 g/dl生理盐水法于0～60 min内检测PTCP血中PLT结果与草酸铵法之间差异均无统计学意义(t=0.694～1.062,P>0.05)。结论 EDTA法、枸椽酸钠法、血细胞稀释液法及0.9 g/dl生理盐水稀释仪器法即刻检测PTCP患者PLT结果与草酸铵法相一致。30 min内枸椽酸钠法和血细胞稀释液法对PTCP患者PLT检测可达到较理想的效果,但仍有少量PLT聚集而导致PLT小幅下降; 0.9 g/dl生理盐水稀释仪器法0～60 min内与草酸铵法检测PLT结果之间无差异性。
Abstract:
Objective To investigate the effects of 0.9 g/dl NaCl diluting instrument method to solve the ethylenediamine tetraacetic aciddipotassium(EDTA)anticoagulant dependency pseudo reduce platelet syndrome(PTCP)feasibility,provides solutions to clinical laboratory PTCP more effective method.Methods From August to October of 2014 in their laboratory for PTCP cases in all 3 cases,2 ml venous blood in EDTA and citron acid sodium anticoagulation in-line blending,in the immediate,10,30,40 and 60min computer detection.Collected of peripheral blood in blood thinners,respectively,0.9 g/dl NaCl solution blending,in the immediate,10,30,40 and 60 mincomputer detection,and compared with the manual method of ammonium oxalate.Results EDTA,citron acid sodium,blood thinners and 0.9 g/dl NaCl diluting instrument immediately detected PTCP blood PLT result comparedwith ammonium oxalate method,there were no statistically significant difference(t=0.943～1.537,P>0.05),10 min～60 min anticoagulant blood PLT resultssignificantly decreased,compared with ammonium oxalate method difference had statistical significance(t=12.413～12.413,P<0.01～0.001).Citron acid sodium PLT began to decline after 30 min,compared with ammonium oxalate method,thedifference was statistically significant(t=4.915～4.915,P<0.05～0.01).Blood dilution method in 30～40 min PLT test results began to decline,but not obvious,there was no statistically significant difference with the method of ammonium oxalate(t=1.315～1.715,P>0.05),40～60 min PLT test results appear significantly decreased,and the method of ammonium oxalate difference was statistically significant(t=3.175～3.175,P<0.05); Within 0～60 min 0.9 g/dl NaCl method to detect the PLT differences between the results with the method ofammonium oxalate had no statistical significance(t=0.694～1.062,P>0.05).Conclusion EDTA,citron acid sodium,blood thinners and0.9 g/dl saline diluting instrument immediately detected PLT PTCP patients were consistent with ammonium oxalate method.Citron acid sodium within 30 minutesand blood dilution method in patients with PTCP PLT detection could achieve ideal effect,but there were still a small amount of PLT gathered and led to a slight drop in PLT.0.9 g/dl saline diluting instrument method with ammonium oxalatewithin 0～60 minutes method to detect the PLT result had no difference.

参考文献/References:

[1] 丛玉隆,王昌富,乐家新.血细胞自动化分析后血涂片复审标准制定的原则与步骤[J].中华检验医学杂志,2008,31(7):729-732. Cong YL,Wang CF,Yue JX.Principles and procedure of determining criteria for smear review following automated complete blood count and leukocyte differential count[J].Chinese Journal of Laboratory Medicne,2008,31(7):729-732.
[2] 宓庆梅,施魏宇,郝婉莹,等.EDTA依赖性假性血小板减少症1例[J].中华检验医学杂志,2004,27(10):719. Mi QM,Shi WY,Hao WY,et al.A case EDTA dependent pseudothrombocytopenia[J].Chinese Journal of Laboratory Medicine,2004,27(10):719.
[3] 张学英,王素平,李玲玲.204例假性血小板减少实验分析[J].国际检验医学杂志,2009,30(2):166-167. Zhang XY,Wang SP,Li LL.204 cases of pseudo thrombocytopenia experimental analysis[J].International Journal of Laboratory Medicine,2009,30(2):166-167.
[4] 叶应妩, 王毓三, 申子瑜.全国临床检验操作规程[S].3版.南京:东南大学出版社,2006:136-143. Ye YW,Wang YS,Shen ZY.The national clinical laboratory operationrules[S].3th Ed.Nanjing:Southeast University Press,2006:136-143.
[5] Carrillo-Esper R,Contreras-Domingnez V.Pseudothr-ombocytopenia inducedby ethylenedi aminetetraacetic acid in burned patients[J].Cir Cir,2004(72):335.
[6] Satoh M,Hirose Y,Gamo M,et al.Sudden onset of EDTA-dependent pseudothrombocytopenia in a patient scheduled for open heart surgery[J].Mazui,2003,52(4):402-405.
[7] Hsieh AT,Chao TY,Chen YC.Pseudothrombocytopenia associated with infectious mononucleosis[J].Arch Patho1 Lab Med,2003(127):17-18.
[8] Kinoshita Y,Yamane T,Kamimoto A,et al.A case of pseudothrombocytopenia during antibiotic administration[J].Rinsho Byori,2004,52(2):120-123.
[9] Tucc H,Yang S.Olanzapine-induced EDTA-dependent pseudothrombocytopenia[J].Psychosomatics,2002,43(5):421-423.
[10] 张建萍.EDTA依赖性假性血小板减少症及检测方法分析[J].重庆医学,2010,39(20):2782-2784. Zhang JP.EDTA dependency pseudo thrombocytopenia and detection method analysis[J].Chongqing Medical,2010,33(20):2782-2784.
[11] 邝妙欢,刘晓华,钟义富,等.EDTA依赖性血小板假性减少症血小板的检测[J].国际检验医学杂志,2010,31(11):1224-1225. Kuang MH,Liu XH,Zhong YF,et al.The analysis of PLT count in EDTA dependent pseudothrombocy openia[J].International Journal of Laboratory Medicine,2010,31(11):1224-1225.
[12] 杨 军. EDTA抗凝剂引起假性血小板减低的分析[J].中国医学研究与临床,2007(5):59-60. Yang J.EDTA anticoagulant cause sham platelet reduce analysis[J].ChinaMedical Research and Clinical,2007(5):59-60.
[13] 邵永生,郑宏伟.组合检验法在EDTA依赖性假性血小板减少症中的应用[J].国际检验医学杂志,2012,33(16):2036-2037. Shao YS,Zheng HW.Combination test in the application of EDTA dependencypseudo thrombocytopenia[J].International Journal of Laboratory Medicine,2012,33(16):2036-2037.
[14] 丛玉隆,尹一兵,陈 瑜.检验医学高级教程[M].北京:人民军医出版社,2010:123. Cong YL,Yin YB,Chen Y.Laboratory medicine advanced tutorial[M].Beijing:People's Military Medical Press,2010:123.
[15] 黄 胜,梁华英,曾梦如,等.EDTA-K2依赖性血小板假性减少现象分析及纠正方法探讨[J].现代检验医学杂志,2011,26(3):136-138. Huang S,Liang HY,Zeng MR,et al.Analysis the phenomenon anticoagulantsEDTA-K2 led to false platelet reducing and research the correcting method[J].Journal Modern Laborator Medical,2011,26(3):136-138.

备注/Memo

备注/Memo:
作者简介:刘爱胜(1973-),男,本科,学士,副主任技师,主要从事临检工作,E-mail:curious1998@163.com。
更新日期/Last Update: 2015-03-20