[1]姚亚洲,郑引索.72例老年急性白血病免疫表型分析[J].现代检验医学杂志,2015,30(05):91-93.[doi:10.3969/j.issn.1671-7414.2015.05.027]
　YAO Ya-zhou,ZHENG Yin-suo.Immunophenotype Analysis of 72 Cases of Elderly Acute Leukemia[J].Journal of Modern Laboratory Medicine,2015,30(05):91-93.[doi:10.3969/j.issn.1671-7414.2015.05.027]
点击复制

72例老年急性白血病免疫表型分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
91-93
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Immunophenotype Analysis of 72 Cases of Elderly Acute Leukemia
作者:
姚亚洲; 郑引索
宝鸡市中心医院血液风湿科，陕西宝鸡721008
Author(s):
YAO Ya-zhou; ZHENG Yin-suo
Department of Blood and Rheumatism，Baoji Central Hospital，Shaanxi Baoji 721008，China
关键词:
白血病; 老年; 免疫表型; 流式细胞术
分类号:
R557；R392.11
DOI:
10.3969/j.issn.1671-7414.2015.05.027
文献标志码:
A
摘要:
目的探讨老年急性白血病流式细胞术免疫分型的特点及临床意义。方法回顾性分析2010年1月～2015年3月，利用三色法流式分析法检测76例老年性白血病的免疫表型及其特点。结果①AML中最主要表达的抗原是MPO，CD13和CD33，表达率分别为98.2％，98.2％和7.2％。47.3％有淋系抗原表达，其中CD7(19.3％)，CD56(26.3％)最为常见。②老年B-ALL中，CD19的表达阳性率最高为100％，其次是cCD79a，CD10和CD20，表达阳性率分别为88.9％，46.2％和66.7%。纯B系抗原表达者77.8%，伴T系抗原表达者22.3%，髓系相关抗原CD13，CD33的表达阳性率分别为33.4%和22.3%。③所有老年T-ALL中均表达CD3与CD7，CD5(+)细胞占66.7%，干祖细胞CD34，HLA-DR阳性率分别为33.3％和0％。结论流式细胞术免疫表型分析是一种简便、快速 、准确的检测技术手段，结合传统的检测手段，有助于老年白血病的精确诊断。
Abstract:
ObjectiveTo explore the flow cytometry(FCM) immune classification of elderly acute leukemic characteristics and clinical significance．MethodsAnalysed retrospectively the immunophenotype and the character of 76 patients with elderly acute leukemia，that performed by 3-color flow cytometric analysis method．Results①The major marker in AML were MPO，CD13，CD33，expression rate of 98.2%，98.2% and 77.2%，respectively．②The lymphoid immunophenotypy marker was 47.3% in all patients，including CD7 (19.3%)，CD56 (26.3%．CD19 expression in all older B-lymphoid leukemia，followed by cCD79a，CD10 and CD20 expression positive rate were 88.9%，46.2% and 66.7% respectively．Pure B antigen expression was 77.8%，with T antigen reaches 22.3%．The expression of myeloid associated antigen CD13 was 33.4%，and CD33 positive rate was 22.3%．③The T-cell special marker CD3 and CD7 were in whole T-ALL，CD5 expression positive rate was 66.7%．Only 33.3% cases expressed progenitor cell marker CD34，no had HLA-DR expression．ConclusionFCM immune phenotype analysis is a simple，rapid and accurate means of detection technology，combined with the traditional detection methods，will effected help accurate diagnosis of senile leukemia．

参考文献/References:

［1］陈芳源．老年白血病的特点及治疗进展［J］．老年医学与保健，2013，19(1)：7-11． Chen FY．Characteristics and treatment of leukemia in elderly［J］．Geriatrics & Health Care，2013，19(1)：7-11．
[2]张之南，沈悌．血液病诊断及疗效标准［M］．2版.北京：北京科学出版社，2007：131-134． Zhang ZN，Shen T．Diagnosis and effect of blood disease［M］．2th Ed.Beijing：Beijing Science Press，2007：131-134．
[3]Weir EG，Borowitz MJ．Flow cytometry in the diagnosis of acute leukemia［J］．Serrin Hematol，2001，38(2)：124-138．
[4]袁莉，杨玉琮，陈葳．93例急性白血病流式细胞术免疫分型的特点分析［J］．现代检验医学杂志，2014，29(2)：127-129． Yuan L，Yang YZ，Chen W．Analysis of immunophenotypic features by flow cytometer in 93 cases with acute leukemia［J］．Journal of Modern Laboratory Medicine，2014，29(2)：127-129．
[5]丁才智．CD34，CD38和HLA-DR表达及其对急性白血病诊治的意义［J］．检验医学与临床，2011，8(20)：2502-2504． Ding CZ．Significance of CD34，CD38 and HLA-DR expression and for diagnosis and treatment of acute leukemia［J］．Laboratory Medicine and Clinic，2011，8(20)：2502-2504．
[6]佟海侠，王慧涵，张继红，等．192例急性髓系白血病免疫表型、细胞遗传学及临床特征的研究［J］．中国实验血液学杂志，2009，17(5)：1174-1178． Tong HX，Wang HH，Zhang JH，et al．Immunophenotyes,cytogenetics and clinical features of 192 patients with acute myeloid leukemia［J］．Journal of Experimental Hematology，2009，17(5)：1174-1178．
[7]戴海滨，姚丹丹，张延清，等．伴淋系抗原表达的急性髓系白血病特点及临床意义［J］．哈尔滨医科大学学报，204，48(3)：206-208． Dai HB，Yao DD，Zhang YQ，et al．Characteristics and clinical significance of acute myeloid leukemia with lymphoid lineage antigen［J］．Journal of Harbin Medical University，204，48(3)：206-208．
[8]满秋红，邱立娟，刘铁强，等．成人急性淋巴细胞白血病免疫表型特点分析［J］．中国体视学与图像分析，2008，13(4)：268-271． Man QH，Qiu LJ，Liu TQ，et al．Immunophenotypes feature analysis of 76 cases of adult acute lymphoblastic leukemia［J］．Chinese Journal of Stereology and Image Analysis，2008，13(4)：268-271．
[9]Vitale A，Guarini A，Ariola C，et al．Absence of prognostic impact of CD13 and／or CD33 antigen expression in adult acute lymphoblastic leukemia．Results of the GIMEMA ALL 0496 tria1［J］．Haematologica，2007，92(3)：342-348．

相似文献/References:

[1]谢圆媛,杨丹芬.老年COPD患者外周血单核细胞TLR2,TLR4的表达及其与炎症因子的关系研究[J].现代检验医学杂志,2015,30(04):80.[doi:10.3969/j.issn.1671-7414.2015.04.022]
　XIE Yuan-yuan,YANG Dan-fen.Study on the Expressions of TLR2,TLR4 in Peripheral Blood Mononuclear Cells and Its Correlation with the Inflammatory Factors Levels of the Elderly Patients with COPD[J].Journal of Modern Laboratory Medicine,2015,30(05):80.[doi:10.3969/j.issn.1671-7414.2015.04.022]
[2]苗玉迪a,魏绪仓b.达沙替尼联合氟达拉滨对慢粒K562细胞的抑制作用研究[J].现代检验医学杂志,2015,30(05):70.[doi:10.3969/j.issn.1671-7414.2015.05.021]
　MIAO Yu-dia,WEI Xu-cangb.Study on Inhibition of Dasatinib and Fludarabine to CML K562 Cells[J].Journal of Modern Laboratory Medicine,2015,30(05):70.[doi:10.3969/j.issn.1671-7414.2015.05.021]
[3]付朝泓,李艳,童永清,等.BCR-ABL融合基因核酸检测试剂的研发及临床应用[J].现代检验医学杂志,2015,30(06):52.[doi:10.3969/j.issn.1671-7414.2015.06.015]
　FU Chao-hong,LI Yan,TONG Yong-qing,et al.Development and Clinical Application of A Novel Nucleic Acid Detection Reagent for BCR-ABL Fusion Gene[J].Journal of Modern Laboratory Medicine,2015,30(05):52.[doi:10.3969/j.issn.1671-7414.2015.06.015]
[4]王结珍,梁培松,王伟佳,等.血清PCT,IL-6和CRP水平检测对白血病患者化疗并发败血症的诊断价值[J].现代检验医学杂志,2019,34(02):88.[doi:10.3969/j.issn.1671-7414.2019.02.023]
　WANG Jie-zhen,LIANG Pei-song,WANG Wei-jia,et al.Diagnostic Value of Serum PCT,IL-6 and CRPin Septicemia Patients with Leukemia in Chemotherapy[J].Journal of Modern Laboratory Medicine,2019,34(05):88.[doi:10.3969/j.issn.1671-7414.2019.02.023]
[5]孟凡萍,郝　坡.渝东北地区441 例白血病患者融合基因表达特点分析[J].现代检验医学杂志,2020,35(02):15.[doi:10.3969/j.issn.1671-7414.2020.02.005]
　MENG Fan-ping,HAO Po.Analysis of Expression Characteristics of Fusion Gene in 441 Patientswith Leukemia in Northeastern Chongqing[J].Journal of Modern Laboratory Medicine,2020,35(05):15.[doi:10.3969/j.issn.1671-7414.2020.02.005]
[6]李彬钰a,马锡慧a,李 蕊a,等.老年新型冠状病毒感染患者外周血淋巴细胞亚群和多种细胞因子水平分析及其临床价值研究[J].现代检验医学杂志,2024,39(02):113.[doi:10.3969/j.issn.1671-7414.2024.02.021]
　LI Binyua,MA Xihuia,LI Ruia,et al.Analysis and Clinical Value of Lymphocyte Subsets and Various Cytokines of Peripheral Blood in Elderly Patients with COVID-19[J].Journal of Modern Laboratory Medicine,2024,39(05):113.[doi:10.3969/j.issn.1671-7414.2024.02.021]
[7]马 骁,吴世林.老年性白内障患者泪液中miR-223 和miR-143-3p 水平表达与术后干眼症的相关性研究[J].现代检验医学杂志,2024,39(03):72.[doi:10.3969/j.issn.1671-7414.2024.03.012]
　MA Xiao,WU Shilin.Correlation between the Expression of miR-223 and miR-143-3p Levels in Tears of Elderly Cataract Patients and Postoperative Dry Eye Syndrome[J].Journal of Modern Laboratory Medicine,2024,39(05):72.[doi:10.3969/j.issn.1671-7414.2024.03.012]

备注/Memo

备注/Memo:
作者简介：姚亚洲(1970-)，男，学士，副主任医师，主要研究方向：血液系统疾病临床研究，E-mail：yaoyazhou001@tom.com。
更新日期/Last Update: 1900-01-01