[1]乔军,刘俊娜,王春娟,等.流式微球术、胶体金渗滤法及ELISA法检测HIV抗体的评价与分析[J].现代检验医学杂志,2015,30(06):104-104.[doi:10.3969/j.issn.1671-7414.2015.06.031]
　QIAO Jun,LIU Jun-na,WANG Chun-juan,et al.Evaluation and Analysis of HIV Antibodies Detected with Flow Microbeads Assay，Dot Immune-gold Filtration Assay and ELISA[J].Journal of Modern Laboratory Medicine,2015,30(06):104-104.[doi:10.3969/j.issn.1671-7414.2015.06.031]
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流式微球术、胶体金渗滤法及ELISA法检测HIV抗体的评价与分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年06期
页码:
104-104
栏目:
研究简报·实验技术
出版日期:
2015-12-30

文章信息/Info

Title:
Evaluation and Analysis of HIV Antibodies Detected with Flow Microbeads Assay，Dot Immune-gold Filtration Assay and ELISA
作者:
乔军1; 刘俊娜1; 王春娟1; 张洁2
1.西安市长安区医院，西安710100；2.陕西省人民医院，西安710068
Author(s):
QIAO Jun1; LIU Jun-na1; WANG Chun-juan1; ZHANG Jie2
1.Changan District Hospital of Xi’an，Xi’an 710100，China； 2.Shaanxi Provincial People's Hospital,Xi’an 710068,China
关键词:
HIV抗体; 流式微球术; 胶体金渗滤法; 酶联免疫吸附试验
分类号:
R446.61
DOI:
10.3969/j.issn.1671-7414.2015.06.031
文献标志码:
A
摘要:
目的探讨采用流式微球术(FMA)，胶体金斑点免疫渗滤法(DIGFA)与ELISA法检测HIV抗体三种试验之关系，为提高艾滋病检测技术提供依据。方法分别采用FMA，DIGFA与ELISA法检测HIV抗体阳性血清标本98例和86例HIV抗体阴性标本。结果FMA与ELISA的K一致性检验，k值=0.911 2，P<0.01，提示两种方法的检测结果具有极强的一致性。FMA与DIGFA的K一致性检验，k值=0.903 2，P<0.01，提示两种方法的检测结果具有极强的一致性。故三种方法的检测结果具有极强的一致性。FMA法检测阳性率为100％，而DIGFA检测阳性率是94％。ELISA法检测阳性率为95％。结论三种试验方法检测的特异度和灵敏度没有显著性差异。
Abstract:
ObjectiveTo explore the relation among adopting FMA，the colloidal gold osmosis of immune systern spots and detecting all HIV antibodies in order to improve HIV testing technology．MethodsAdopted three different methods，FMA，the osmosis of immune systern spots of colloidal gold and detecting all HIV antibodies to test 98 positive serum samples and 86 negative serum samples．ResultsThe detection of K consistency of FMA and ELISA was：k=0.911 2，P<0.01．Using the two methds，found that the two kinds of test results were extremely consistent．The detection result of K consistency of FMA and DIGFA was：k=0.903 2，P<0.01 which shows that test results were extremely consistent．So three methods could lead to the extreme consistency．By using FAM could assure that the positive rate of detection was 100%．By using the colloidal gold osmosis，found that the positive rate of detection was 94%．By using ELISA the positive rate was 95%．ConclusionThere were no significant differences in detecting the specificity and sensitivity．

参考文献/References:

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备注/Memo

备注/Memo:
作者简介：乔军(1964-)，女，副主任检验技师，从事临床检验及医院管理工作。 通讯作者：张洁，女，研究员，E-mail：zhangjie810@aliyun.com.cn。
更新日期/Last Update: 1900-01-01