[1]马丽颜,梁 静,周建龙.腺瘤样结肠息肉易感基因(APC)及结直肠癌缺失基因(DCC)基因甲基化在肺癌早期诊断的意义[J].现代检验医学杂志,2016,31(01):17-20.[doi:10.3969/j.issn.1671-7414.2016.01.005]
　MA Li-yan,LIANG Jing,ZHOU Jian-long.Evaluation of the Combination of APC and DCC Gene Methylation in the Early Diagnosis of Lung Cancer[J].Journal of Modern Laboratory Medicine,2016,31(01):17-20.[doi:10.3969/j.issn.1671-7414.2016.01.005]
点击复制

腺瘤样结肠息肉易感基因(APC)及结直肠癌缺失基因(DCC)基因甲基化在肺癌早期诊断的意义()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
17-20
栏目:
论著
出版日期:
2016-01-25

文章信息/Info

Title:
Evaluation of the Combination of APC and DCC Gene Methylation in the Early Diagnosis of Lung Cancer
作者:
马丽颜; 梁 静; 周建龙
广州军区广州总医院,广州 510010
Author(s):
MA Li-yan; LIANG Jing; ZHOU Jian-long
General Hospital of Guangzhou Military Command,Guangzhou 510010,China
关键词:
肺癌;  腺瘤样结肠息肉易感基因;  结直肠癌缺失基因;  甲基化
分类号:
R735.35; R730.43
DOI:
10.3969/j.issn.1671-7414.2016.01.005
文献标志码:
A
摘要:
目的 探讨腺瘤样结肠息肉易感基因(APC)及结直肠癌缺失基因(DCC)基因甲基化在肺癌早期诊断的意义。方法 对245例肺癌患者、150例非恶性肺病患者和40例健康志愿者抽取晨起空腹外周血,应用甲基化特异性PCR法检测APC和DCC启动子区甲基化状态。并对其与临床病理特征等进行相关性分析。结果 ①肺癌组患者外周血中APC及DCC启动子甲基化阳性率分别为26.53%(65/245)和36.33%(89/245); 非肺癌组患者外周血中APC及DCC启动子甲基化阳性率分别为2.67%(4/150)和8.00%(12/150); 健康志愿者外周血中APC及DCC启动子甲基化阳性率为0; 肺癌组与非肺癌组、健康志愿者组相比,APC和DCC基因甲基化检测结果比较,差异均有统计学意义(P<0.01)。②APC及DCC基因甲基化联合检测,诊断肺癌灵敏度为52.65%,特异度为89.33%,联合检测与单独APC检测相比,敏感度与特异度差异均有统计学意义(P<0.01); 联合检测与单独DCC检测相比,敏感度有统计学差异(P<0.01),但是特异度差异不明显(P>0.05); ③甲基化检测结果与患者性别、年龄、病理类型、病理分化程度、临床TNM分期等没有相关性(P>0.05)。结论 APC和DCC基因甲基化与非小细胞肺癌的发生发展密切相关,可以作为肺癌早期诊断标志物之一。
Abstract:
Objective To investigate the significanceof both the adenomatous polyposis coli(APC)and deleted in colorectal carcinoma(DCC)gene methylation in the early diagnosis of lung cancer.Methods 245 patients with lung cancer and 150 patients with non-malignant lungdisease patients and 40 healthy volunteers were drawn for the experiment.A methylation specific PCR(MSP)was used to detect the methylation status of APC andDCC in the peripheral blood.Results The positive rates of APC and DCC genes promoter methylation Peripheral blood of patients with lungcancer were 26.53%(65/245)and 36.33%(89/245),respectively.The positive rates of APC and DCC genes promoter methylation Peripheral blood of patients withbenign lung diseases were 2.67%(4/150),8.00%(12/150),respectively.The positive rates of APC and DCC genes promoter methylation Peripheral blood of healthyvolunteers were 0.There was a significant difference between patients with thelung cancer,those with the benign lung diseases and Healthy volunteers(P<0.01).The sensitivity and specificity of lung cancer was respectively 52.65%and 89.33% by the combination of APC and DCC genes methylation.Comparing withthe separate APC detection,sensitivity and specificity statistical differenceswere significant in joint measurement(P<0.01).Comparing with the separateDCC detection,sensitivity statistical differences were significant,but specificity statistical differences was not obvious(P>0.05).There was no correlation between methylation test results and patient gender,age,pathological type,pathological grade,TNM stage and so on(P>0.05).ConclusionAPC and DCC gene methylation were closely related to the development of non-small cell lung cancer.They can be used as an early diagnostic markerof lung cancer.

参考文献/References:

[1] Matsuda A, Katanoda K. Five-year relative survival rate of lung cancer in the USA,Europe and Japan[J].Japan J Clin Oncol,2013,43(12):1287-1288.
[2] Kwon YJ,Lee SJ,Koh JS,et al.Genome-wide analysis of DNA methylation and the gene expression change in lung cancer[J].Journal of Thoracic Oncology,2012,7(1):20-33.
[3] 高 丽,潘世扬,陈 丹,等.熔解曲线法用于肺癌APC基因甲基化模式的研究[J].现代检验医学杂志,2012,27(1):19-23. Gao L,Pan SY,Chen D,et al.Investigation of methylation patternsof APC gene in lung cancer with a novel fluorescence metlting curve analysis assay[J].Journal of Modern Laboratory Medicine,2012,27(1):19-23.
[4] Zysman M,Saka A,Millar A,et al.Methylation of adenomatous polyposis coli in endometrial cancer occurs more frequently in tumors with microsatellite instability phenotype[J].Cancer Res,2002,62(13):3663-3666.
[5] 宋 超,张 云,金永堂,等.非小细胞肺癌患者血浆DAPK基因甲基化检测及其临床意义[J].肿瘤防治研究,2013,40(8):772-775. Song C,Zhang Y,Jin YT,et al.Detection and clinical significanceof DAPK gene methylation in plasma of not-small cell lung cancer patients[J].Cancer Research on Prevention and Treatment's,2013,40(8):772-775.
[6] Ostrow KL,Hoque MO,Loyo M,et al.Molecular analysis of plasma DNA forthe early detection of lung cancer by quantitative methylation-specific PCR[J].Clin Cancer Res,2010,16(13):3463-3472.
[7] Miao Y,Wang L,Zhang X,et al.Promoter methylation-mediated silencing of β-catenin enhances invasiveness of non-small cell lung cancerand predicts adverse prognosis[J].PLoS One,2014,9(11):e112258.
[8] Wu F,Lu M,Qu L,et al.DNA methylation of hMLH1 correlates with the clinical response to cisplatin after a surgical resection in non-small cell lung cancer[J].Int J Clin Exp Pathol,2015,8(5):5457-5463.
[9] 王 虹,李丽丽,张吉才,等.5-氮杂-2'脱氧胞苷对肺癌A549/DDP细胞hMLHl,MGMT基因甲基化及其表达的影响[J].现代检验医学杂志,2015,30(3):83-86. Wang H,Li LL,Zhang JC,et al.Effects of 5-Aza-Cde on DNA methylation and expression of hMLHl and MGMT gene in lung cancer cell line A549/DDP[J].Journal of Modern Laboratory Medicine,2015,30(3):83-86.
[10] Juergens RA,Wrangle J,Vendetti FP,et al.Combination epigenetic therapy has efficacy in patients with refractory advanced non-small cell lung cancer[J].Cancer Discovery,2011,1(7):598-607.

相似文献/References:

[1]彭 瑛,邓正华,温先勇.国内13种血清肿瘤标志物对肺癌诊断价值的Meta分析[J].现代检验医学杂志,2016,31(01):96.[doi:10.3969/j.issn.1671-7414.2016.01.028]
　PENG Ying,DENG Zheng-hua,WEN Xian-yong.Diagnostic Value of Thirteen Types of Serum Tumor Markers for Lung Cancer in China:A Meta-Analysis[J].Journal of Modern Laboratory Medicine,2016,31(01):96.[doi:10.3969/j.issn.1671-7414.2016.01.028]
[2]翟海军,朱晶.红细胞分布宽度对肺癌预后的临床分析[J].现代检验医学杂志,2015,30(06):147.[doi:10.3969/j.issn.1671-7414.2015.06.045]
　ZHAI Hai-jun,ZHU Jing.Clinical Analysis of Red Blood Cell Distribution Width on the Prognosis of Lung Cancer[J].Journal of Modern Laboratory Medicine,2015,30(01):147.[doi:10.3969/j.issn.1671-7414.2015.06.045]
[3]姜 波,王玉明,段 勇.肺癌组织中调控PUMA基因 的miRNA筛查和MiR-221表达差异研究[J].现代检验医学杂志,2015,30(01):15.[doi:10.3969/j.issn.1671-7414.2015.01.005]
　JIANG Bo,WANG Yu-ming,DUAN Yong.Study on the Differential Expression of miRNA Screening and MiR-221 Regulates Apoptosis by Targeting PUMA Gene in Lung Cancer[J].Journal of Modern Laboratory Medicine,2015,30(01):15.[doi:10.3969/j.issn.1671-7414.2015.01.005]
[4]赵车冬,闫玉珠,吴文婧,等.血清CEA,CYFRA21-1,NSE水平在肺癌诊断与分型中的应用价值[J].现代检验医学杂志,2016,31(06):59.[doi:10.3969/j.issn.1671-7414.2016.06.016]
　ZHAO Che-dong,YAN Yu-zhu,WU Wen-jing,et al.Application Value of Serum CEA,CYFRA21-1 and NSE Levels in the Diagnosis and Typing of Lung Cancer[J].Journal of Modern Laboratory Medicine,2016,31(01):59.[doi:10.3969/j.issn.1671-7414.2016.06.016]
[5]董 莉,张保平,冯新平,等.血浆HSP90α检测在肺癌诊断中的临床应用价值研究[J].现代检验医学杂志,2017,32(04):97.[doi:10.3969/j.issn.1671-7414.2017.04.027]
　DONG Li,ZHANG Bao-ping,FENG Xin-ping,et al.Plasma HSP90 Alpha Testing Clinical Application Value in the Diagnosis of Lung Cancer Researc[J].Journal of Modern Laboratory Medicine,2017,32(01):97.[doi:10.3969/j.issn.1671-7414.2017.04.027]
[6]张利祥,潘 静,赵 洁,等.痰液基细胞学检查与血清CYFRA21-1,CEA,NSE联合检测对肺癌的诊断价值[J].现代检验医学杂志,2019,34(03):99.[doi:10.3969/j.issn.1671-7414.2019.03.024]
　ZHANG Li-xiang,PAN Jing,ZHAO Jie,et al.Value of the Combined Determination of Sputum Liquid-Based Cytologyand Serum CYFRA21-1,CEA,NSE for the Diagnosis of Lung Cancer[J].Journal of Modern Laboratory Medicine,2019,34(01):99.[doi:10.3969/j.issn.1671-7414.2019.03.024]
[7]樊　华,弥　鹏,杨　波,等.肺癌患者外周血血小板参数检测分析及临床意义[J].现代检验医学杂志,2019,34(06):97.[doi:10.3969 ／ j.issn.1671-7414.2019.06.024]
　FAN Hua,MI Peng,YANG Bo,et al.Analysis and Clinical Value of Peripheral Platelet Parameters in Lung Cancer Patients[J].Journal of Modern Laboratory Medicine,2019,34(01):97.[doi:10.3969 ／ j.issn.1671-7414.2019.06.024]
[8]赵格a,高琼b,余宗涛a,等.肺癌患者血浆miR-3151 表达水平与临床病理特征的相关性研究[J].现代检验医学杂志,2020,35(05):28.[doi:10.3969/j.issn.1671-7414.2020.05.008]
　ZHAO Gea,GAO Qiongb,YU Zong-taoa,et al.Study on the Correlation between the Expression of miR-3151 in Plasma and Clinicopathological Features in Patients with Lung Cancer[J].Journal of Modern Laboratory Medicine,2020,35(01):28.[doi:10.3969/j.issn.1671-7414.2020.05.008]
[9]毛　俊,王　柯.老年肺癌患者肺切除术后血液 CRP，D- 二聚体，IL-17 水平与并发急性呼吸衰竭的相关性研究[J].现代检验医学杂志,2021,36(03):83.[doi:10.3969/j.issn.1671-7414.2021.03.019]
　MAO Jun,WANG Ke.Correlation of Blood CRP, D-dimer and IL-17 Levels with Acute RespiratoryFailure in Elderly Patients with Lung Cancer after Pneumonectomy[J].Journal of Modern Laboratory Medicine,2021,36(01):83.[doi:10.3969/j.issn.1671-7414.2021.03.019]
[10]李　辉,汪春新,秦明明,等.肺癌患者血清7项肿瘤标志物联合检测在病理分型及临床分期中的应用价值研究[J].现代检验医学杂志,2021,36(04):5.[doi:10.3969/j.issn.1671-7414.2021.04.002]
　LI Hui,WANG Chun-xin,QIN Ming-ming,et al.Study on the Application Value of Combined Detection of 7 Tumor Markersin Serum of Patients with Lung Cancer in Pathological Classificationand Clinical Staging[J].Journal of Modern Laboratory Medicine,2021,36(01):5.[doi:10.3969/j.issn.1671-7414.2021.04.002]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81401752)。 作者简介:马丽颜(1985-),女,本科学历,硕士在读,技师,主要从事肿瘤的诊断研究,Tel:13570308378。 通讯作者:周建龙,男,主要从事肿瘤的诊断和治疗,Tel:13189090119。
更新日期/Last Update: 2016-01-20