[1]黄璐,刘丽华,庄运芳,等.MN血型基因分型应用于河源地区临床输血检测[J].现代检验医学杂志,2016,31(06):130-132.[doi:10.3969/j.issn.1671-7414.2016.06.038]
　HUANG Lu,LIU Li-hua,ZHUANG Yun-fang,et al.Genotying of MN Blood Group Applied to Clinical Blood Transfusion in Heyuan Area[J].Journal of Modern Laboratory Medicine,2016,31(06):130-132.[doi:10.3969/j.issn.1671-7414.2016.06.038]
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MN血型基因分型应用于河源地区临床输血检测()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
130-132
栏目:
检验与临床
出版日期:
2016-12-20

文章信息/Info

Title:
Genotying of MN Blood Group Applied to Clinical Blood Transfusion in Heyuan Area
作者:
黄璐1; 刘丽华1; 庄运芳1; 陈丽红1; 欧小懂1; 梁延连2
黄 璐1,刘丽华1,庄运芳1,陈丽红1,欧小懂1,梁延连2
Author(s):
HUANG Lu1; LIU Li-hua1; ZHUANG Yun-fang1; CHENLi-hong1; OU Xiao-dong1; LIANG Yan-lian2
1.Heyuan Blood Center,Guangdong Heyuan 517000,China; 2.Shenzhen Blood Center,Guangdong Shenzhen 518035,China
关键词:
MN血型;  基因分型;  河源地区;  临床输血
分类号:
R457.11
DOI:
10.3969/j.issn.1671-7414.2016.06.038
文献标志码:
A
摘要:
目的 应用基因分型技术鉴定产生抗-M抗体的患者样本MN血型,协助解决MN血型相关的临床输血问题。方法 在血清学鉴定的基础上,采用聚合酶链反应-序列特异性引物(PCR-SSP)基因定型方法对14例产生抗-M抗体样本的MN血型进行基因分型,并与血清学结果比较; 选取M抗原阴性的血液与之进行交叉配血。结果 14例样本基因分型结果与血清学分型结果相符,其中11例为NN型,2例为MN型,1例为MM型。选取M抗原阴性的供血者血液与该14例患者血液配血均相合,患者输血后无输血反应,24 h内血红蛋白上升。结论 MN血型基因分型技术可作为血清学方法的补充,有利于临床输血相关疑难样本的检测。
Abstract:
Objective To help solve the problem of clinicalblood transfusion related to MN blood group,using genotyping technology to identify MN blood group of the patients' blood sample which exist anti-M antibody.Methods A total of 14 blood samples which exist anti-Mantibody were detected for MN blood group by polymerase chain reaction-sequence specific primers(PCR-SSP)and serologic technique,respectively,and compared the genotyping results with serotyping results.Selected M antigen negative blood for cross matching with 14 cases.Results 14 cases'sgenotyping results and serotyping results were comcidental and in them,NN typeof 11 cases,MN type of 2 cases,MM type of 1 cases.The M antigen negative blood cross matched with 14 cases were compatible.The patients had no blood transfuse reaction after blood transfusion,and the hemoglobin were increase within 24hour.Conclusion Genotyping technology is helpful for detecting complicated samples related to clinical blood transfusion.It can be usefor the compliment of serologic technique.

参考文献/References:

[1] 杰夫·丹尼尔(英).人类血型[M].2版.朱自严,译.北京:科学出版社,2007:172. Daniels G.Human Blood Groups[M].Second edition.Zhu ZY,Translation.Beijing:Science Press,2007:172.
[2] Lee E,Burgess G,Halverson GR,et al.Applications of murine and humanized chimaeric monoclonal antibodies for red cell phenotyping[J].Br J Haematol,2004,126(2):277-281.
[3] 许德意,孙春霞,高家良,等.MN血型四种方法检测比较分析[J].中国优生与遗传杂志,2015,23(8):37-39. Xu DY,Sun CX,Gao JL,et al.Comparative analysis of four kinds ofmethods to detect the MN blood group[J].Chinese Journal of Birth Health & Heredity,2015,23(8):37-39.
[4] 叶应妩,王毓三,申子瑜.全国临床检验操作规程[S].3版.南京:东南大学出版社,2006:260-261. Ye YW,Wang YS,Shen ZY.National Guide to Clinical Laboratory Procedures[S].Third Edition.Nanjing:Southeast University Press,2006:260-261.
[5] Reid ME.MNS blood group system:a review[J].Immunohematology,2009,25(3):95-101.
[6] 王钰箐,蔡晓红,龚淞颂,等.46 346名患者不规则抗体筛查结果及分析[J].中国输血杂志,2015,28(8):1004-1006. Wang YQ,Cai XH,Gong SS,et al.Result analysis of irregular antibody screening[J].Chin J Blood Transfusion,2015,28(8):1004-1006.
[7] 李 惠,徐焕铭,张 毅,等.输血前患者不规则抗体筛查及鉴定结果分析[J].中国实验血液学杂志,2015,23(3):861-865. Li H,Xu HM,Zhang Y,et al.Analysis of patients` irregular antibody screening and identification results before blood transfusion[J].Journal of Experimental Hematology,2015,23(3):861-865.
[8] 王晓华,樊 晶.备血患者不规则抗体筛查及其结果分析[J].中国输血杂志,2015,28(12):1513-1515. Wang XH,Fan J.Analysis of irregular antibody scre-ening results in patients prepared for blood transfusion[J].Chin J Blood Transfusion,2015,28(12):1513-1515.
[9] 黄 璐,陈丽红,庄运芳,等.广东省河源地区MN血型基因频率调查[J].国际医药卫生导报,2014,20(14):2052-2054. Huang L,Chen LH,Zhuang YF,et al.Gene frequencies of MN blood group in Heyuan,Guangdong[J].International Medicine and Health Guidance News,2014,20(14):2052-2054.
[10] 陈云龙,梁延连,苏宇清,等.人类红细胞MN血型中抗-M与GYPA基因表达的相关性[J].临床输血与检验,2014,16(1):8-10. Chen YL,Liang YL,Su YQ,et al.A study of correlation of anti-M production and the GYPA expression in human MN blood type[J].J Clin Transfus Lab Med,2014,16(1):8-10.
[11] 梁延连,苏宇清,吴 凡,等.中国人群MN血型中M,N等位基因多态性的研究[J].中国实验血液学杂志,2015,23(2):537-540. Liang YL,Su YQ,Wu F,et al.Polymorphism of M,N allele in MN blood group of Chinese population[J].Journal of Experimental Hematology,2015,23(2):537-540.

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备注/Memo

备注/Memo:
基金项目:广东河源地区MN血型的基因研究与临床输血应用(2013-066)。 作者简介:黄 璐(1974-),女,副主任护师,主要研究方向为采供血管理,Tel:0762-3374999,E-mail:874469381@qq.com。
更新日期/Last Update: 2016-12-20