[1]黄生金,郑利平,陆俊佳,等.广西地区壮族和汉族冠心病患者胱抑素C水平及其基因多态性的比较研究[J].现代检验医学杂志,2017,32(03):26-29，33.[doi:10.3969/j.issn.1671-7414.2017.03.007]
　HUANG Sheng-jin,ZHENG Li-ping,LU Jun-jia,et al.Comparative Study of Cystatin C and Its Gene Polymorphism of Pati ents with Coronary Heart Disease between Zhuang and Han in Guangxi Region[J].Journal of Modern Laboratory Medicine,2017,32(03):26-29，33.[doi:10.3969/j.issn.1671-7414.2017.03.007]
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广西地区壮族和汉族冠心病患者胱抑素C水平及其基因多态性的比较研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
26-29，33
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Comparative Study of Cystatin C and Its Gene Polymorphism of Pati ents with Coronary Heart Disease between Zhuang and Han in Guangxi Region
文章编号:
1671-7 414(2017)03-026-05
作者:
黄生金1; 郑利平2; 陆俊佳2; 朱 旭2
1.南宁市马山县人民医院医学检验科，南宁 530600；
2.南宁市第二人民医院医学检验科，南宁 530031
Author(s):
HUANG Sheng-jin1; ZHENG Li-ping2; LU Jun-jia2 ; ZHU Xu2
1.Department of Clinical Laboratory，the People’s Hospital of Mashan，Nanning 530600， China；
2.Department of Clinical Laboratory，the Second People’s Hospital of Nanning，Nanning 530031，China
关键词:
胱抑素C; 基因多态性; 壮族; 冠心病
分类号:
R541.4；R446.11
DOI:
10.3969/j.issn.1671-7414.2017.03.007
文献标志码:
A
摘要:
目的 探讨广西地区壮族冠心病患者血清中胱抑素C(Cys C) 水平及其基因位点+148 G/A和-82 G/C的多态性与同地区汉族冠心病(CHD)患者及两个民族 正常人的不同。方法 采用免疫比浊法检测壮族、汉族CHD患者和壮 族、汉族正常个体(各100例)血清中Cys C水平，聚合酶链反应-限制性片段长度多态性(PCR -RFLP)技术检测Cys C基因位点+148和-82的多态性，并分析上述数据和临床资料。〖HT5 ”H〗结果 ①两组CHD组血清中Cys C水平和临床资料与两组正常组间的差异有 统计学意义(P值均<0.05)；②四组中Cys C基因位点+148和-82等位基因分布频率符合 H-W 群体遗传平衡法则(P值均>0.05)，具有群体代表性。CHD组Cys C基因+148 和-8 2位点不同基因型分布频率较同民族正常组虽有差异，但差异均无统计学意义(χ2=0.7 60～2.090，P> 0.05)；③CHD病组血清中Csy C水平与Cr呈正相关(r=0.597，P<0 .001)。结论 Cys C基因位点+148 和-82的多态性与广西地区的壮 族、汉族CHD相关性需作进一步研究，但肾损伤引起的高Cys C血清水平可能是广西地区壮族 、汉族CHD患者的一个危险因素。
Abstract:
Objective To investigate the difference of seru m levels of cystatin C and its gene polymorphism of patients with CHD and normal people between Zhuang and Han in Guanxi region．Methods The levels of serum cystatin C in Zhuang CHD patients，Han CHD patients，Zhuang normal people and Han normal people(each of 100 cases)were detected by Immunotur bidimetric Assays，Cys C+148 and Cys C-82 genotypes were conducted by using PCR -RFLP，and that data and clinical data were analyzed．Results 〖WT5 ”BZ〗The difference of Cys C levels and clinical data between two CHD groups a nd two normal groups were statically significant (all P<0.05)．①The Cys C levels of two CHD groups was not statically significant difference (P=0.156 )．②The CysC+148 and Cys C-82 of 4 groups conform the Hardy-Weinberg populati on genetic equilibrium law (all P>0.05)．The genotypes frequency difference of Cys C+148 and Cys C-82 between two CHD groups and two normal group were no statically significant (χ2=0.760～2.090，P>0.05)．③The serum Cys C le vel and Cr of CHD group were positively correlated (r=0.597，P<0.001)．〖W T5”HZ〗Conclusion The correlation Cys C+148，-82 polymorphism and Guangxi Zhuang CHD were in need of further study，but the kidney damage caused b y the high serum Cys C level may be a risk factor for Guangxi Zhuang and Han CHD patients．

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备注/Memo

备注/Memo:
基金项目：广西壮族自治区南宁市科技课题资助(编号：201 2137)。
作者简介：黄生金(1964-)，男，本科学历，副主任检 验技师，主要研究方向临床医学检验，Tel：13237719025,E-mail：huangshengjin1964@12 6.com。
通讯作者：朱 旭(1961-)，男，本科学历，主任检验技师，主要 研究方向分子生物诊断学，Tel：0771-2246516。
更新日期/Last Update: 2017-06-25