[1]荆 晶,胡恩亮,樊爱琳,等.低MCV引起PLT计数假性增高的实验研究[J].现代检验医学杂志,2018,33(02):108-110.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　JING Jing,HU En-liang,FAN Ai-lin,et al.Analysis of the Influence of Different MCV Level on the Platelet False Increase Counts[J].Journal of Modern Laboratory Medicine,2018,33(02):108-110.[doi:10.3969/j.issn.1671-7414.2018.02.001]
点击复制

低MCV引起PLT计数假性增高的实验研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
108-110
栏目:
研究简报·实验技术
出版日期:
2018-04-05

文章信息/Info

Title:
Analysis of the Influence of Different MCV Level on the Platelet False Increase Counts
文章编号:
1671-7414(2018)02-108-03
作者:
荆 晶; 胡恩亮; 樊爱琳; 郑善銮; 郝晓柯
空军军医大学西京医院检验科,西安 710032
Author(s):
JING Jing; HU En-liang; FAN Ai-lin; ZHENG Shan-luan; HAO Xiao-ke
Department of Clinical Laboratory, Xijing Hospital,the Air Force Military Medical University,Xi'an 710032,China
关键词:
血小板计数;  小红细胞;  红细胞碎片;  红细胞平均体积
分类号:
R446.111
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 评价低红细胞平均体积(MCV)对PLT计数的影响,为实验室制定PLT复检规则提供依据。方法 随机收集2017年3～8月期间的466例门诊检测血细胞计数及五分类患者EDTA抗凝血标本,检验结果分为三组:A组MCV≤65fl,B组65fl<MCV≤70fl,C组70fl<MCV≤75fl。采用SYSMEX-XN9000血细胞分析仪分别进行电阻抗法(PLT-I)和荧光核酸染色法(PLT-F)血小板计数,将两种方法检测的血小板计数结果进行统计分析。结果 A组PLT-I值=(322.8±109.1)×109/L,PLT-F值=(282.60±100.5)×109/L,二者之间差异有统计学意义(t=6.799,P<0.05); B组PLT-I值=(305.7±111.7)×109/L,PLT-F值=(304.8±112.3)×109/L; C组 PLT-I值=(292.2±84.4)×109/L,PLT-F值=(291.6±84.4)×109/L。结论 当存在小红细胞或红细胞碎片的情况下,即低MCV≤65fl时,电阻抗法(PLT-I)检测PLT结果,会引起PLT计数的假性增高。
Abstract:
Abstract:Objective To evaluate the influence of the meancorpuscular volume(MCV)in different ranges on PLT counts,provide the theoretical basis for making PLT counts review rules in laboratory.MethodsFrom March 2016 to August 2016 in Xijing Hospital department of outpatient who perform complete blood cell count were randomly divided into 3 groups,MCV≤65 fl in A group,65 fl<MCV≤70 fl in B group and 70 fl<MCV≤75 fl in Cgroup.The reference method(Coulter principle)compared with the fluorescence method,accuracy analysis of different monitoring methods of counting PLT.Results PLT-I and PLT-F count values in A group was(322.8±109.1)×109/L and(282.60±100.5)×109/L respectively,and there was significant differences between the two groups(t=6.799,P<0.05).In B group,the count values was(305.7±111.7)×109/L and(304.8±112.3)×109/L respectively,and there was no differences between two groups.In C group,thecount values was(292.2±84.4)×109/L and(291.6±84.4)×109/L respectively,and there was nodifferences between two groups neither.Conclusion Whensmall red blood cells or blood cell debris present in blood circulation,MCV≤65fl,the reference testing(Coulter principle)of platelets causes a false increase in platelet count.

参考文献/References:

[1] 段 浩,陈 锋,顾 彪,等.血细胞分析技术及其进展研究[J].医疗卫生装备,2014,35(5):108-112. Duan H,Chen F,Gu B,et al.Study of hematology analysis technologyand its development[J].Chinese Medical Equipment Journal,2014,35(5):108-112.
[2] 肖木洲,张广聪,叶竟妍,等.全自动血细胞分析仪对形态异常血细胞检测功能的评价[J].广州中医药大学学报,2006,23(3):225-227. Xiao MZ,Zhang GC,Ye JY,et al.Evaluation of automatic blood cellanalyzer for detecting abnormal morphologic cells[J].Jounal of Guangzhou University of Traditional Chinese Medicine,2006,23(3):225-227.
[3] 郭玉娥,赵洪泉,赵志明.血细胞分析仪计数血小板的影响因素[J].中国误诊学杂志,2005,5(1):152-153. Guo YE,Zhao HQ,Zhao ZM.The influence factor of counting plateletsby blood cell analyzer[J].Chinese Journal of Misdiagnostics,2005,5(1):152-153.
[4] 陈言伟,王朝鑫.光学法检测大血小板的临床应用价值分析[J].实验与检验医学,2014,32(5):643-644. Chen YW,Wang CX.Analysis of clinical application value of opticalmethod for detecting large platelets[J].Experimental and Laboratory Medicine,2014,32(5):643-644.
[5] 李少芳,韦 敏.用电阻抗法自动血细胞分析仪检测血小板的影响因素研究[J].当代医药论丛,2017,15(12):110-111. Li SF,Wei M.Study on the influencing factors of platelet detectionby electrical impedance automatic blood cell analyzer[J].Contemporary Medicine Forum,2017,15(12):110-111.
[6] 崔颖鹏,陈维安,陈冬梅,等.两种常见妊娠合并症的血小板参数变化分析[J].实用医学杂志,2003,19(3):312-313. Cui YP,Chen WA,Chen DM,et al.Analysis of platelet parameters intwo types of common pregnency complications[J].The Journal of Practical Medicine,2003,19(3):312-313.
[7] Sawaidis A,Schuett K.Platelet fuction[J],Der Diabetologe,2016,12(2):102-108.
[8] Sasaki K,Kawai K,Tsuno NH,et al.Impact of preoperative thrombocytosison the survival of patients with primary colorectal cancer[J].World Journalof Surgery,2012,36(1):192-200.
[9] 梁 巍,周艳丽,应媛媛,等.不同检测方法对大血小板计数的对比与心脑血管疾病的临床价值[J].中国实用神经疾病杂志,2016,19(17):60-61. Liang W,Zhou YL,Ying YY,et al.Comparison of large platelet countby different detection methods and clinical value of cardiovascular and cerebrovascular diseases[J].Chinese Journal of Practical Neruous Diseases,2016,19(17):60-61.
[10] 陈 娟,董 晶,彭赛辉,等.PLT-F计数低值血小板的准确性评价[J].实验与检验医学,2016,34(3):288-290,293. Chen J,Dong J,Peng SH,et al.Accuracy evaluation of PLT-F method on low concentration of platelet counting[J].Experimental and Laboratory Medicine,2016,34(3):288-290,293.
[11] 赵秀丽,黄晓燕,万振洲.XN-9000血液分析仪“低值血小板”检测通道的临床应用评价[J].临床输血与检验,2016,18(6):603-605. Zhao XL,Huang XY,Wan ZZ.Clinical application evaluation of new fluorecsent method(PLT-F)on Sysmex XN-9000 hematology analyzer achieved higher accuracy in low platelet counting[J].Jounral of Clinical Transfusion and LaboratoryMedicine,2016,18(6):603-605.
[12] 徐文丽,李 舟.XN-9000血细胞分析仪3种计数血小板方法结果差异分析[J].临床血液学杂志,2017,30(2):122-125. Xu WL,Li Z.Differential analysis of the results of three kinds of platelet counting methods in XN-9000 sysmex blood cell analyzer[J].Journal of Clinical Hematology,2017,30(2):122-125.
[13] Wada A,Takagi Y,Kono M,et al.Accuracy of a new platelet count system(PLT-F)depends on the staining property of its reagents[J].PLoS One,2015,10(10):e141311.
[14] 胡文静,周荣富,贾 璐,等.不同血液病患者发生自发性出血事件时的血小板特征[J].江苏医药,2016,42(11):1258-1260. Hu WJ,Zhou RF,Jia L,et al.Charactoristics of platelet in patients withdifferent kinds of hematonosis during spontaneous bleeding[J].Jiangsu Medical Journal,2016,42(11):1258-1260.

相似文献/References:

[1]樊　凡,唐西怀,何浩浩.外周血血小板计数与红细胞比容联合检测对代谢综合征的预测价值[J].现代检验医学杂志,2020,35(06):125.[doi:doi:10.3969/j.issn.1671-7414.2020.06.030]
　FAN Fan,TANG Xi-huai,HE Hao-hao.Predictive Values of Combined Detection of Peripheral Blood PlateletCount and Hematocrit for Metabolic Syndrome[J].Journal of Modern Laboratory Medicine,2020,35(02):125.[doi:doi:10.3969/j.issn.1671-7414.2020.06.030]
[2]汪学耀,荆成宝,张　倩,等.静脉血真空采血管预稀释法校正 EDTA 依赖性假性血小板减少的效果探讨[J].现代检验医学杂志,2020,35(06):156.[doi:doi:10.3969/j.issn.1671-7414.2020.06.038]
　WANG Xue-yao,JING Cheng-bao,ZHANG Qian,et al.Discussion on the Efficacy of Prediluted Vacuum Blood Collection TubeMethod to Correct EDTA-Dependent Pseudothrombocytopenia[J].Journal of Modern Laboratory Medicine,2020,35(02):156.[doi:doi:10.3969/j.issn.1671-7414.2020.06.038]

备注/Memo

备注/Memo:
作者简介:荆晶(1988-),女,医学硕士,主管技师,主要从事临床血液学及体液学工作,E-mail:sevencee@sina.com。 通讯作者:郝晓柯,男,教授,主任医师,博士生导师。 荆 晶,胡恩亮,樊爱琳,郑善銮,郝晓柯(空军军医大学西京医院检验科,西安 710032)
更新日期/Last Update: 2018-04-16