[1]王亚南.标本溶血对固相免疫层析法检测降钙素原结果影响的实验研究[J].现代检验医学杂志,2018,33(04):127-130.[doi:10.3969/j.issn.1671-7414.2018.04.035]
　WANG Ya-nan.Experimental Study on the Effect of Sample Hemolysis on the Detection of Procalcitonin by Solid Phase Immunochromatography Assay[J].Journal of Modern Laboratory Medicine,2018,33(04):127-130.[doi:10.3969/j.issn.1671-7414.2018.04.035]
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标本溶血对固相免疫层析法检测降钙素原结果影响的实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
127-130
栏目:
研究简报·实验技术
出版日期:
2018-08-30

文章信息/Info

Title:
Experimental Study on the Effect of Sample Hemolysis on the Detection of Procalcitonin by Solid Phase Immunochromatography Assay
文章编号:
1671-7414(2018)04-127-04
作者:
王亚南
苏州市立医院 南京医科大学附属苏州医院检验科,江苏苏州 215002
Author(s):
WANG Ya-nan
Department of Clinical Laboratory,Suzhou Municipal Hospital, the Affiliated Suzhou Hospital of Nanjing Medical University,Jiangsu Suzhou 215002,China
关键词:
降钙素原;  溶血;  固相免疫层析法
分类号:
Q503
DOI:
10.3969/j.issn.1671-7414.2018.04.035
文献标志码:
A
摘要:
目的 评估标本溶血对固相免疫层析法检测降钙素原(procalcition,PCT)结果的影响。方法 收集2016年11月无溶血标本共66例,其中PCT<0.5 ng/ml组为22例,0.5 ng/ml≤PCT<10 ng/ml组为22例,PCT≥10 ng/ml组22例。将66例无溶血标本制备成不同血红蛋白浓度(0,3,6,9,12,15 g/L)的样本,离心后取50 μl血浆,采用固相免疫层析法检测PCT值,计算经稀释因子校正后PCT值,分析不同溶血程度的标本对PCT结果的影响。结果 0.5 ng/ml≤PCT<10 ng/ml组中,当血红蛋白浓度为12,15 g/L时,PCT值与对照组相比差异有统计学意义(t=6.71,P=0.000 1; t=6.91,P=0.000 0),PCT值是溶血前PCT值的65%和56%。PCT≥10ng/ml组中,当血红蛋白浓度为9,12,15 g/L时,PCT值与对照组相比差异有统计学意义(t=4.04,P=0.000 2; t=6.91,P=0.000 0; t=4.04,P=0.000 2),PCT值是溶血前PCT值的0.73%,0.61%和0.53%。结论 溶血对PCT检测有干扰作用,使得PCT值假性偏低。医务人员应做好分析前质量控制,尽量避免标本溶血。
Abstract:
Abstract:Objective To explore the effect of clinical hemolytic specimens on the detection of procalcitonin by solid phase immunochromatography.Methods 66 cases of samples without hemolysis were collected in Nov 2016,included 22 cases in group PCT<0.5 ng/ml,22 cases ingroup 0.5 ng/ml ≤PCT<10 ng/ml and 22 cases in group PCT≥10 ng/ml.66 cases ofsamples without hemolysis were prepared into samples with different hemoglobinconcentration(0,3,6,9,12,15 g/L).After centrifugation,50 μl plasma wastaken and PCT value was detected by solid phaseimmunochromatography assay.PCT value corrected by dilution factor was calculated.The effect of different hemolysis degree on PCT results was analyzed.Results In 0.5 ng/ml≤PCT<10 ng/ml group,when the concentration of hemoglobin was 12,15 g/L,PCT value had significant difference compared with the control group(t=6.71,P=0.000 1; t=6.91,P=0.000 0).PCT value was 65% and 56% compared before hemolysis.In PCT≥10 ng/ml group,when the concentration of hemoglobin was9,12 and 15 g/L,PCT value had significant difference compared with the controlgroup(t=4.04,P=0.000 2; t=6.91,P=0.000 0; t=4.04,P=0.000 2).PCT value was 73%,61% and 53% compared before hemolysis.ConclusionHemolysis had a disturbing effect on PCT detection,making PCT false low.The medical staff should do a good job of quality control before analysis,and try to avoid the hemolysis of the specimens.

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备注/Memo

备注/Memo:
作者简介:王亚南(1980-),女,博士在读,副主任技师,主要从事临床检验研究,E-mail:wangyn1980@163.com。
更新日期/Last Update: 2018-08-16