[1]黄映红,林 涛,陈卓瑶.儿童重型β珠蛋白生成障碍性贫血患者外周血淋巴细胞亚群和免疫球蛋白水平状态的研究[J].现代检验医学杂志,2019,34(02):23-26.[doi:10.3969/j.issn.1671-7414.2019.02.007]
　HUANG Ying-hong,LIN Tao,CHEN Zhuo-yao.Clinical Signification of Peripheral Blood Lymphocyte Subsets andSerum Immunoglobin Determination in Children with β Thalassemia Major[J].Journal of Modern Laboratory Medicine,2019,34(02):23-26.[doi:10.3969/j.issn.1671-7414.2019.02.007]
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儿童重型β珠蛋白生成障碍性贫血患者外周血淋巴细胞亚群和免疫球蛋白水平状态的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年02期
页码:
23-26
栏目:
论著
出版日期:
2019-05-11

文章信息/Info

Title:
Clinical Signification of Peripheral Blood Lymphocyte Subsets andSerum Immunoglobin Determination in Children with β Thalassemia Major
文章编号:
1671-7414(2019)02-023-04
作者:
黄映红; 林 涛; 陈卓瑶
(广州市妇女儿童医疗中心检验科,广州 510120)
Author(s):
HUANG Ying-hong; LIN Tao; CHEN Zhuo-yao
(Department of Clinical Laboratory,Guangzhou Municipal Women and Children' Medical Center,Guangzhou 510120,China)
关键词:
儿童;  β珠蛋白生成障碍性贫血;  淋巴细胞亚群;  免疫球蛋白;  免疫功能
分类号:
R556.61; R392.11
DOI:
10.3969/j.issn.1671-7414.2019.02.007
文献标志码:
A
摘要:
目的 研究重型β珠蛋白生成障碍性贫血患儿外周血淋巴细胞各亚群、血清免疫球蛋白(Ig)水平的变化,并探讨其在重型β珠蛋白生成障碍性贫血患儿输血治疗过程中的意义。方法 采用流式细胞术(FCM)和免疫比浊法,对45例重型β珠蛋白生成障碍性贫血患儿和50例健康对照组儿童外周血T淋巴细胞亚群(包括CD3+,CD3+CD4+,CD3+CD8+和CD4+/CD8+)、B淋巴细胞亚群(CD3-CD19+)、NK细胞(CD16+CD56+)和血清Ig(IgG,IgA和IgM)水平进行检测,两组间进行比较。结果 与健康组相比较,重型β珠蛋白生成障碍性贫血患儿CD3+T细胞比例[(60.49±9.23)% vs(68.41±6.31)%,t=2.46,P=0.005],CD4+T细胞比例[(31.19±5.02)% vs(43.23±4.52)%,t=3.31,P=0.002],CD4+/CD8+比值[(1.19±0.31)% vs(1.73±0.27)%,t=3.78,P=0.001]、NK细胞[(9.27±2.24)% vs(18.04±5.07)%,t=3.92,P=0.001]比例明显减低,差异均具有统计学意义; 而CD8+T细胞比例[(29.11±4.03)% vs(24.49±5.13)%,t=1.89,P=0.04]和CD3-CD19+B细胞比例[(26.13±2.99)% vs(12.53±3.11)%,t=4.45,P=0.001]明显增高,差异均具有统计学意义。与此同时,与健康组相比较,重型β珠蛋白生成障碍性贫血患儿血清IgG(13.27±2.31 g/L vs 11.41±1.81 g/L,t=2.93,P=0.011),IgA(1.99±0.43 g/L vs 1.19±0.19 g/L,t=2.69,P=0.043)和IgM(1.91±0.45 g/L vs 1.49±0.27 g/L,t=2.81,P=0.02)水平明显增高,差异均具有统计学意义。结论 重型β珠蛋白生成障碍性贫血患儿体内存在着细胞和体液免疫功能紊乱。外周血淋巴细胞亚群和免疫球蛋白水平状态的测定,对监测患儿输血治疗过程中机体免疫功能状态的变化有重要意义。
Abstract:
Objective To investigate the changes of peripheral blood lymphocyte subsets and serum immunoglobin(Ig)in children with β thalassemiamajor,and to explore the clinical signification during their course oftransfusiontherapy.Methods Peripheral blood lymphocyte subsets including CD3+,CD4+CD8+,CD19+ and CD16+CD56+ were determined by flow cytometry and serumimmunoglobin levels including IgG,IgA and IgM were detrmined by nethelometry assay in a total of 45 children with β thalassemia major and 50 healthy controls were enrolled.The differences between the β thalassemia major group and healthy control group were analyzed statistics.Results Compared with control group,the peripheral blood lymphocyte subsets CD3+,CD3+CD4+,CD3+CD8+and NK cell were significantlydecreased in β thalassemia major group [(60.49±9.23)% vs(68.41±6.31)%,t=2.46,P=0.005],[(31.19±5.02)% vs(43.23±4.52)%,t=3.31,P=0.002],[(1.19±0.31)% vs(1.73±0.27)%,t=3.78,P=0.001] and [(9.27±2.24)% vs(18.04±5.07)%,t=3.92,P=0.001],but CD8+ and CD19+ were significantly increased in β thalassemia major group [(29.11±4.03)% vs(24.49±5.13)%,t=1.89,P=0.04]and[(26.13±2.99 )% vs(12.53±3.11)%,t=4.45,P=0.001].Meanwhile,the levels of serum IgG,IgA and IgM in β thalassemia major group(13.27±2.31 g/L vs 11.41±1.81 g/L,t=2.93,P=0.011),(1.99±0.43 g/L vs 1.19±0.19 g/L,t=2.69,P=0.043),(1.91±0.45 g/L vs 1.49±0.27 g/L,t=2.81,P=0.020)were significantly increased.Conclusion The cellular and humeral immune function in children with β thalassemia major were abnormal.The determination of peripheral lymphocyte subsets and immunoglobin should be used to monitor the changes of immune function during their course of transfusion therapy.

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备注/Memo

备注/Memo:
基金项目:广东省医学科学技术研究基金(A2017585)。 作者简介:黄映红(1980-),女,本科,学士学位,主管技师,主要从事临床检验医学,E-mail:ying8037@sina.com。 收稿日期:2018-12-25 修回日期:2019-03-05
更新日期/Last Update: 2019-04-30