[1]李 娜a,赵晓娟b,苏晓明a.乳腺组织中SMG-1mRNA和SOX4mRNA检测在乳腺癌监测中的应用[J].现代检验医学杂志,2019,34(02):35-39.[doi:10.3969/j.issn.1671-7414.2019.02.010]
　LI Naa,ZHAO Xiao-juanb,SU Xiao-minga.Application of Detection of SMG-1mRNA and SOX4mRNAin Breast Tissue in Surveillance for Breast Cancer[J].Journal of Modern Laboratory Medicine,2019,34(02):35-39.[doi:10.3969/j.issn.1671-7414.2019.02.010]
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乳腺组织中SMG-1mRNA和SOX4mRNA检测在乳腺癌监测中的应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年02期
页码:
35-39
栏目:
论著
出版日期:
2019-05-11

文章信息/Info

Title:
Application of Detection of SMG-1mRNA and SOX4mRNAin Breast Tissue in Surveillance for Breast Cancer
文章编号:
1671-7414(2019)02-035-05
作者:
李 娜a; 赵晓娟b; 苏晓明a
(咸阳市第一人民医院,a.检验科; b.妇产二科,陕西咸阳 712000)
Author(s):
LI Naa; ZHAO Xiao-juanb; SU Xiao-minga
(a.Department of Laboratory; b.the Second Department ofGynaecologyand Obstetrics,the First People's Hospital of Xianyang City,Shaanxi Xianyang 712000,China)
关键词:
乳腺癌;  浸润性导管癌;  非典型导管增生;  SMG-1mRNA;  SOX4mRNA;  CA153;  CEA
分类号:
R737.9; R730.43
DOI:
10.3969/j.issn.1671-7414.2019.02.010
文献标志码:
A
摘要:
目的 探讨组织生殖器形成抑制基因-1(suppressor withmorphogenetic effect on genitalia-1,SMG-1)mRNA和SOX4mRNA检测在乳腺癌监测中的应用价值。方法 回顾性分析2008年5月～2015年5月于咸阳市第一人民医院就诊的45例乳腺浸润性导管癌(IDC组)患者和32例乳腺非典型导管增生(ADH组)患者的临床资料,同时选择30例癌旁正常乳腺组织作为对照组,应用实时逆转录-聚合酶链反应(RT-PCR)技术检测组织SMG-1mRNA和SOX4mRNA的表达,比较分析两标志物的表达与乳腺癌的关系。结果 组织SOX4mRNA在对照组、ADH组和IDC组中的异常率和表达水平分别为20.0%,53.1%,77.7%和0.21±0.07,0.57±0.13,0.89±0.18; 组织SMG-1mRNA在三组中的异常率和表达水平分别为83.3%,60.0%,26.6%和0.92±0.21,0.62±0.12,0.25±0.09。与对照组比较,组织SOX4mRNA在ADH组与IDC组中的异常率和表达水平均显著增高,而SMG-1mRNA则显著降低; 与ADH组比较,组织SOX4mRNA在IDC组中的异常率和表达水平显著增高,而SMG-1mRNA则显著降低; 以上比较的差异均有统计学意义(F表达水平=326.23～353.97,χ2异常率=4.51～24.24,P<0.01或P<0.05)。血清CA153在对照组、ADH组和IDC组中的异常率和水平分别为3.3%,25.0%,44.4%和13.2±11.3,50.3±36.2,132.0±77.9 μg/ml; 血清CEA在三组中的异常率和表达水平分别为3.3%,12.5%,20.0%和2.3±1.6,19.3±7.6,47.3±31.9 ng/ml。与ADH组和对照组比较,血清CA153和CEA水平在IDC组中显著增高,其差异有统计学意义(χ2=5.37～5.47,均P<0.01)。在45例乳腺癌患者中,组织SMG-1mRNA和SOX4mRNA表达具有负相关性(r=-0.832,P<0.01)。SMG-1mRNA表达与血清CA153和CEA水平有负相关性(r=-0.767,-0.623,P<0.01),而SOX4mRNA则有正相关性(r=0.779,0.605,P<0.01)。组织SOX4mRNA的表达与乳腺癌的肿瘤大小和淋巴结转移具有显著相关性(χ2=5.37～5.47,均P<0.05)。SMG-1mRNA表达与乳腺癌的肿瘤大小、TNM分期、淋巴结转移及组织学分级均有相关性(均χ2=4.60～6.03,P<0.05)。结论 检测组织SMG-1mRNA和SOX4mRNA表达可应用于乳腺癌的早期发现,降低乳腺癌的漏检率。
Abstract:
Objective To investigate the value of SMG-1 mRNA and SOX4 mRNA in monitoring breast cancer.Methods The clinical data of 45 patients with invasive ductal carcinoma(IDC group)and 32patients with atypical ductal hyperplasia(ADH group)were retrospectively analyzed between May 2008 and May 2015 in the First People's Hospital of XianyangCity,and 30 cases of normal breast tissue adjacent to cancer were as control group.The expression of SMG-1 mRNA and SOX4 mRNA were detected by RT-PCR,and compared and analyzed the relationship with breast cancer.Results The positive rate and expression level of tissue SOX4 gene in control group,ADH group and IDC group were 20.0%,53.1%,77.7% and 0.21±0.07,0.57±0.13,0.89±0.18,respectively.And those of tissue SMG-1 gene in three groups were 83.3%,60.0%,26.6% and 0.92±0.21,0.62±0.12,0.25±0.09,respectively.Compared with those in the control group,the positive rate and expression of SOX4 mRNA were significantly increased and which of SMG-1 mRNA were significantly decreased in ADH and IDC groups.In addition,these similar presenses of SOX4 mRNA and SMG-1 mRNA had been described in the IDC group compared with in the ADH group(Fexpression=326.33～353.97,χabnormalrate=4.51～24.24,P<0.001 or 0.05).The positive rate and the expression level of serum CA153 in the control group,ADH group and IDC group were 3.3%,25.0%,44.4% and 13.2±11.3,50.3±36.2,132.0±77.9 μg/ml,respectively,and those of serum CEA in the third group were 3.3%,12.5%,20.0% and 2.3±1.6,19.3±7.6,47.3±31.9 ng/ml,respectively.The serumal levels of CA153 and CEA were observed to be significantly higher in the IDC group than those in the control and ADHgroups(P<0.01).In 45 cases of the patiens with breast cancer,the expression of SMG-1 mRNA and SOX4 mRNA was found to be negatively correlated(r=-0.832,P<0.01).Furthermore,here,showed these negative correlations between SMG-1 mRNA expression and serum levels of CA153 and CEA(r=-0.767,-0.623,P<0.01,respectively),and these positive correlations between SOX4 mRNA expression and them(r=0.779,0.605,P<0.01,respectively).The expression of SOX4 in the breast cancer tissues was significantly correlated with the size of tumor and lymph node metastasis(χ2=5.37～5.47,all P<0.05).The expression of SMG-1 was correlated with tumor size,TNM stage,lymph node metastasis,and histological grade for the breast cancer(χ2=4.60～6.03,all P<0.05).Conclusion Detecting SMG-1 mRNA and SOX4 mRNA expression in breast cancer tissues can be applied to discovery the early breast cancer and improve the positive detection rate of breast cancer.

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备注/Memo

备注/Memo:
作者简介:李 娜(1982-),女,本科学历,主管检验师,研究方向:肿瘤标志物的检测,E-mail:2634085933@qq.com。 通讯作者:苏晓明(1980-),女,本科学历,主管检验师,研究方向:乳腺肿瘤的诊断,E-mail:912188145@qq.com。 收稿日期:2018-12-10 修回日期:2019-01-07
更新日期/Last Update: 2019-04-30