[1]曼热帕·吐尔逊,马　蓉,祖菲娅·艾力.干细胞转录因子 Oct4及 Sox2对宫颈癌细胞成瘤、迁移和浸润能力的影响[J].现代检验医学杂志,2022,37(01):145-148.[doi:10.3969/j.issn.1671-7414.2022.01.029]
　MANREPA ?Tuerxun,MA Rong,ZUFEIYA?Aili.Effects of Stem Cell Transcription Factors Oct4 and Sox2 on the Occurrence, Migration and Invasion of Cervical Cancer Cells[J].Journal of Modern Laboratory Medicine,2022,37(01):145-148.[doi:10.3969/j.issn.1671-7414.2022.01.029]
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干细胞转录因子 Oct4及 Sox2对宫颈癌细胞成瘤、迁移和浸润能力的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
145-148
栏目:
论　著
出版日期:
2022-01-15

文章信息/Info

Title:
Effects of Stem Cell Transcription Factors Oct4 and Sox2 on the Occurrence, Migration and Invasion of Cervical Cancer Cells
文章编号:
1671-7414（2022）01-145-04
作者:
曼热帕·吐尔逊; 马　蓉; 祖菲娅·艾力
（新疆医科大学第一附属医院妇科中心，乌鲁木齐 　830000）
Author(s):
MANREPA ?Tuerxun;  MA Rong;  ZUFEIYA?Aili
（Department of Gynecology, the First Affiliated Hospital of Xinjiang Medical University，Urumchi 830000，China）
关键词:
肿瘤干细胞; Oct4; Sox2; Transwell侵袭实验; 成球实验
分类号:
R737.33；R730.43
DOI:
10.3969/j.issn.1671-7414.2022.01.029
文献标志码:
A
摘要:
目的　探讨 Oct4和 Sox2对宫颈癌细胞（以下简称 HeLa细胞）成瘤、迁移和浸润能力的影响。方法　复苏并培养 HeLa细胞，分别构建 Oct4及 Sox2过表达质粒及空载质粒，采用脂质体方法转染至 HeLa细胞，按转染质粒的不同分为 4组（正常对照组、 Oct4过表达组、 Sox2过表达组和过表达空载组），铺至 6孔板中，每组一个孔（细胞量为 1×106），转染后 48h进行 PCR验证，转染成功后采取细胞划痕实验检测细胞迁移能力、 Transwell检测细胞侵袭能力及成球实验检测细胞成瘤能力，并采用统计学方法对各组间差异进行分析。结果　PCR验证结果显示， Oct4及 Sox2过表达组 HeLa细胞中 Oct4（5 376±208.7，2.429±0.181 6）和 Sox2（133±23.1，1 991 842±210 370）表达显著高于空载组（2.182±0.518 3, 69.27±11.94）及对照组（0.858 2±0.157 9, 0.793 2±0.213 4）,差异均有统计学意义（F=662.8，
Abstract:
Objective　To investigate the effects of Oct4 and Sox2 on tumorigenesis, migration and infiltration of human cervicalcancer cells (hereinafter referred to HeLa cells ). Methods　The HeLa cells were resuscitated and cultured, and the Oct4 andSox2 overexpression plasmids and empty plasmids were constructed respectively. The transfected cells were transfected intoHeLa cells by liposome method and divided into four groups (normal control group, Oct4 overexpression group, Sox2overexpression group, overexpression no-load group) according to the different transfected plasmids and spread into six-wellplates, where the cell volume per group was 1×106. PCR verification was performed 48h after transfection. After successfultransfection, cell migration ability was detected by cell scratch test, and cell invasion ability and tumorigenesis ability weredetected by Transwell test. The differences between groups were analyzed using statistical methods. Results　PCR verificationresults showed that the overexpression of Oct4 and Sox2 in HeLa cells （5 376±208.7, 2.429±0.1816）and Sox2（133±23.1,1 991 842±210 370） were significantly higher than those in the no-load group（2.182±0.518 3, 69.27±11.94） and thecontrol group（0.858 2±0.157 9, 0.793 2±0.213 4）, the differences were statistically significant (F=662.8, 89.64, P<0.000 1),indicating that the transfection was successful. The HeLa scratch test results showed that the mobility （25.00±5.56） and Sox2Sox2（27.00±1.73） of the Oct4 overexpression group were lower than those in the no-load group（36.67±0.57） and thecontrol group （35.67±0.57）, the difference was statistically significant (F=12.21, P< 0.05). The results of HeLa invasionexperiments showed that the number of invasions (378.3±97.59) and Sox2(374.3±43.89) expressions in the Oct4overexpression group were significantly lower than those in the no-load group（552.7±41.48） and the controlgroup（515.7±57.4）, the difference was statistically significant (F=6.22, P < 0.05). The number of Oct4 overexpressingconstituent spheres (8.667±2.082) and Sox2(8.333±1.155) in HeLa cells in the pelletization experiment were lower than thosein the no-load group （14.67±2.517）and the control group（13.67±1.528），the difference was statistically significant (F=9.11,P<0.05). Conclusion　As transcription factors of tumor stem cells, Oct4 and Sox-2 can inhibit the invasion, metastasis andgrowth of cervical cancer cells, thus providing a new direction for the early treatment and prevention of cervical cancer.

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备注/Memo

备注/Memo:
基金项目：省部共建中亚高发病成因与防治国家重点实验室开放课题青年课题(SKL-HIDCA-2018-20)。
作者简介：曼热帕? 吐尔逊（1986-）女，硕士研究生，主治医师，讲师，研究方向 ：妇科肿瘤，E-mail：lixue198003@126.com。
通讯作者：祖菲娅? 艾力。
更新日期/Last Update: 1900-01-01