[1]彭 浩,张印星,谢 环.长链非编码RNA CDKN2BAS 在子宫内膜癌组织表达及其生物学功能研究[J].现代检验医学杂志,2023,38(03):92-96.[doi:10.3969/j.issn.1671-7414.2023.03.016]
　PENG Hao,ZHANG Yin-xing,XIE Huan.Expression and Biological Function of Long Non-coding RNA CDKN2BAS in Endometrial Carcinoma[J].Journal of Modern Laboratory Medicine,2023,38(03):92-96.[doi:10.3969/j.issn.1671-7414.2023.03.016]
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长链非编码RNA CDKN2BAS 在子宫内膜癌组织表达及其生物学功能研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年03期
页码:
92-96
栏目:
论著
出版日期:
2023-05-15

文章信息/Info

Title:
Expression and Biological Function of Long Non-coding RNA CDKN2BAS in Endometrial Carcinoma
文章编号:
1671-7414（2023）03-092-05
作者:
彭 浩1; 张印星1; 谢 环2
（1. 黄石市中心医院/ 湖北理工学院附属医院妇产科，湖北黄石 435000；2. 宜昌市第二人民医院/ 三峡大学第二人民医院妇产科，湖北宜昌 443000）
Author(s):
PENG Hao1;  ZHANG Yin-xing1;  XIE Huan2
（1. Department of Obstetrics and Gynecology, Huangshi Central Hospital /Affiliated Hospital of Hubei Polytechnic University, Hubei Huangshi 435000, China; 2. Department of Obstetrics and Gynecology, Yichang Second People’s Hospital/the Second People’s Hos
关键词:
子宫内膜癌; 长链非编码RNA-CDKN2BAS; 细胞增殖; 细胞侵袭
分类号:
R737.33；R730.43
DOI:
10.3969/j.issn.1671-7414.2023.03.016
文献标志码:
A
摘要:
目的 探讨长链非编码RNA CDKN2BAS（long non-coding RNA CDKN2BAS，LncRNA CDKN2BAS）在子宫内膜癌（endometrial cancer，EC）组织表达，以及沉默该基因对人EC 细胞HEC-1B 生物学功能的影响。方法 收集2018 年3 月～ 2021 年3 月在黄石市中心医院接受手术治疗的107 例EC 患者资料，实时荧光定量聚合酶链反应(qRTPCR)检测癌组织和癌旁组织中LncRNA CDKN2BAS 表达。培养HEC-1B 细胞，分成siRNA 组、si-NC 组和C 组，分别转染LncRNA CDKN2BAS 抑制序列、对照序列和不作任何处理，并分别采用qRT-PCR，MTT 实验和Transwell 实验检测细胞中LncRNA CDKN2BAS 表达及其对HEC-1B 细胞增殖活性、迁移和侵袭力影响。结果 EC 组织中LncRNACDKN2BAS 表达量为2.73±0.25，高于癌旁组织中的1.00±0.13，差异具有统计学意义（t=63.903，P<0.001）；LncRNA CDKN2BAS在FIGO 分期Ⅲ～Ⅳ期（2.83±0.25）、组织学分级G3级（2.85±0.24）和出现淋巴结转移（2.84±0.26）的EC 组织中的表达量较Ⅰ～Ⅱ期（2.67±0.23），G1 ～ G2 级（2.67±0.22）和未出现淋巴结转移（2.68±0.22）的EC 组织明显升高（t=3.246，3.894，3.270，均P<0.05）；siRNA 组细胞中LncRNA CDKN2BAS 表达量低于si-NC 组和C 组（0.30±0.12 vs 1.02±0.10,1.01±0.12），差异具有统计学意义（F=77.210，P<0.05）；siRNA 组细胞24，48，72 和96h 时A 值低于si-NC 组和C 组，差异具有统计学意义（F=5.003 ～ 8.495，均P<0.05）；siRNA 组迁移细胞数和侵袭细胞数低于si-NC 组和C 组，差异具有统计学意义（F=21.956，22.407，均P<0.05）。结论 EC 组织中LncRNACDKN2BAS 表达量升高，沉默人EC 细胞HEC-1B 中LncRNA CDKN2BAS 表达可抑制细胞增殖活性，减少细胞迁移和侵袭数量。
Abstract:
Objective To explore the expression of long non-coding RNA CDKN2BAS (LncRNA CDKN2BAS) in endometrial cancer (EC) tissues, and the effect of silencing this gene on the biological functions of human EC cells HEC-1B. Methods The data of 107 cases of EC patients who underwent surgical treatment in Huangshi Central Hospital were collected from March 2018 to March 2021, and qRT-PCR was used to detect the expression of LncRNA CDKN2BAS in the cancer tissues and adjacent tissues. HEC-1B cells was cultured and divided into siRNA group, si-NC group and C group, and transfected with LncRNA CDKN2BAS inhibitory sequence, control sequence and without any treatment, respectively. The qRT-PCR, MTT experiment and Transwell experiment were used to detect the expression of LncRNA CDKN2BAS in cells and its effect on the cell proliferation activity, migration and invasion of HEC-1B cells. Results The expression level of LncRNA CDKN2BAS in EC tissues was 2.73±0.25, which was higher than 1.00±0.13 in the adjacent tissues ，the difference was statistically significant(t=63.903, P<0.001). The expression levels of LncRNA CDKN2BAS in EC tissues with FIGO stage III ～ IV(2.83±0.25), histological grade G3(2.85±0.24) and lymph node metastasis(2.84±0.26) were significantly higher than those in EC tissues with stage I ～ II(2.67±0.23), G1 ～ G2(2.67±0.22) and no lymph node metastasis(2.68±0.22) (t=3.246 ～ 3.894, all P<0.05). The expression level of LncRNA CDKN2BAS in the cells in the siRNA group was lower than that in the si-NC group and the C group （0.30±0.12 vs 1.02±0.10,1.01±0.12）, the difference was statistically significant (F=77.210, P<0.05). And the A values of cells at 24, 48, 72 and 96h in the siRNA group were lower than those in the si-NC group and the C group，the differences were statistically significant (F=5.003 ～ 8.495, all P<0.05). The number of migrating cells and the number of invasive cells in the siRNA group were lower than those in the si-NC group and the C group，the differences were statistically significant (F=21.956， 22.407, all P<0.05). Conclusion The expression level of LncRNA CDKN2BAS in EC tissues was increased. Silencing the expression of LncRNA CDKN2BAS in human EC cells HEC-1B could inhibit cell proliferation activity and reduce the number of cell migration and invasion.

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备注/Memo

备注/Memo:
基金项目： 湖北省卫生厅科研基金（WJ2019H524）：高危型HPV 的整合状态对宫颈癌细胞TRAIL 凋亡的影响；2020 年度湖北陈孝平科技发展基金面上项目：利用差速贴壁法分离成年小鼠卵巢生殖干细胞。
作者简介：彭浩（1986-），女，硕士，副主任医师，研究方向：妇产科，E-mail：penghao5234@163.com。
通讯作者：张印星（1981-），男，本科，副主任医师，研究方向：妇产科，E-mail：33414649@qq.com。
更新日期/Last Update: 2023-05-15