[1]董旭才,李 斌,曹聪聪,等.临床实验室单标本气动传输系统改进对血清NSE 和Cyfra21-1 检测结果的影响及效果评价[J].现代检验医学杂志,2023,38(04):169-172.[doi:10.3969/j.issn.1671-7414.2023.04.031]
　DONG Xucai,LI Bin,CAO Congcong,et al.Effect and Evaluation of Single Specimen Pneumatic Transmission System Improvement on Serum NSE and Cyfra21-1 Detection in Clinical Laboratory[J].Journal of Modern Laboratory Medicine,2023,38(04):169-172.[doi:10.3969/j.issn.1671-7414.2023.04.031]
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临床实验室单标本气动传输系统改进对血清NSE 和Cyfra21-1 检测结果的影响及效果评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年04期
页码:
169-172
栏目:
研究简报·实验技术
出版日期:
2023-07-15

文章信息/Info

Title:
Effect and Evaluation of Single Specimen Pneumatic Transmission System Improvement on Serum NSE and Cyfra21-1 Detection in Clinical Laboratory
文章编号:
1671-7414（2023）04-169-04
作者:
董旭才1; 李 斌1; 曹聪聪1; 马 倩1; 王 娇1; 曾宪飞1; 2
（1. 西安区域医学检验中心，西安710116；2. 西北大学医学院，西安710069）
Author(s):
DONG Xucai1;  LI Bin1;  CAO Congcong1;  MA Qian1;  WANG Jiao1;  ZENG Xianfei1; 2
（1.Xi’an Area Medical Laboratory Center, Xi’an 710116, China；2.School of Medicine, Northwest University, Xi’an 710069, China）
关键词:
临床实验室气动传输系统; 神经元特异性烯醇化酶; 细胞角蛋白19 片段抗原21-1
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2023.04.031
文献标志码:
A
摘要:
目的　探讨临床实验室单标本气动传输系统改进对血清神经元特异性烯醇化酶（neuron-specific enolase，NSE）和细胞角蛋白19 片段抗原21-1(cytokeratin 19 fragment antigen21-1,Cyfra21-1）检测结果的影响及效果评价。方法　2021年随机化抽样取60 例员工并采集两管血液标本分为气动传输组（A）和人工转运组（B），以B 组为对照组。A 组通过单标本气动传输系统，B 组通过人工转运同时进行NSE 和Cyfra21-1 检测。2022 年随机化抽样取60 例员工，前30 例员工用干燥管采集两管血液标本分为改进后干燥管气动传输组（C）、改进后干燥管人工转运组（D）。前31 ～ 60 例员工用分离胶管采集两管血液标本分为改进后分离胶管气动传输组（E）、改进后分离胶管人工转运组（F）。离心后C 组和E 组通过单标本气动传输系统，D 组和F 组通过人工转运，四组标本同时进行NSE 和Cyfra21-1 检测，以D 组和F 组为对照组。使用配对t 检验比较六组NSE 和Cyfra21-1 检测结果之间差异并评价存在差异的结果偏倚是否为临床所允许。结果　A 组与B 组的NSE（20.79±6.97ng/ml，14.31±4.10ng/ml）, Cyfra21-1（1.630±0.777ng/ml，1.793±0.786ng/ml）之间结果差异有统计学意义（t=5.672，-5.698，均P<0.05），C 组（15.04±3.60 ng/ml，12.85±1.71ng/ml） 和D 组（1.703±0.156ng/ml，1.797±0.161ng/ml）之间结果差异有统计学意义（t=4.529，-4.260，均P<0.05）。E 组和F 组的NSE（12.94±1.62ng/ml，12.88±1.57ng/ml）结果差异无统计学意义（t=0.308，P>0.05），Cyfra21-1（1.660±0.154ng/ml，1.771±0.159ng/ml）差异有统计学意义（t=-5.169，P<0.05）。NSE 使用干燥管偏倚大于可接受范围，而Cyfra21-1 使用干燥管和分离胶管偏倚均可接受。结论　标本使用分离胶管离心后进行气动传输系统转运对NSE 结果无影响。干燥管和分离胶管进行气动传输系统转运对Cyfra21-1 有一定影响但可以接受。气动传输系统改进后可以减少对NSE 结果的影响。
Abstract:
Objective　To explore the influence of single-specimen pneumatic transmission system improvement in the clinical laboratory on the test results of serum neuron-specific enolase ( NSE) and cytokeratin 19 fragment (Cyfra21-1).Methods　In 2021，60 employees were randomly sampled from two tubes of blood samples collected and divided into pneumatic transfer group (A) and manual transfer group (B), with group B as the control group. Group A passed by the single-specimen pneumatic transport system, and Group B passed by manual transport and simultaneous detection NSE and Cyfra21-1.In 2022,60 employees were randomly sampled, the first 30 employees who collected two tubes of blood samples with drying tubes were divided into two groups: the pneumatic transmission group of the improved drying tube (C) and the manual transport group of the improved drying tube (D). The first 31 ～ 60 employees who collected two tubes of blood samples with the separation hose were divided into the improved pneumatic transfer group (E) and the improved manual transfer group (F). After centrifugation, group C and E were transferred by single-specimen pneumatic transmission, group D and F were transferred manually. NSE and Cyfra21-1 were detected simultaneously in the four groups, with group D and F as the control group. The paired t-test was used to compare the differences between the six groups of NSE and Cyfra21-1 results, and to evaluate whether bias in the results with differences was clinically acceptable. Results　The results of NSE （20.79±6.97ng/ml，14.31±4.10ng/ml）and Cyfra21-1（1.630±0.777ng/ml, 1.793±0.786ng/ml）in group A and B were significantly different (t=5.672，-5.698，all P <0.05).There was a statistically significant difference between group C（15.04±3.60 ng/ml，12.85±1.71ng/ml） and D（1.703±0.156ng/ml，1.797±0.161ng/ ml） (t=4.529，-4.260，all P <0.05). There was no significant difference in NSE （12.94±1.62ng/ml，12.88±1.57ng/ml） between group E and F（t=0.308，P>0.05）, and Cyfra21-1（1.660±0.154ng/ml，1.771±0.159ng/ml） showed statistically significant difference（t=-5.169，P<0.05）. NSE had a greater than acceptable bias using drying tubes, while Cyfra21-1 bias were accepted using both drying tubes and separating hose tubes. Conclusion　The results of NSE were not affected by the pneumatic transport system after centrifugation with the separation hose. Cyfra21-1 would be affected by the transfer of drying pipe and separating hose by the pneumatic transmission system, but it was acceptable. The improved pneumatic transmission system can reduce the impact on NSE results.

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备注/Memo

备注/Memo:
作者简介： 董旭才（1987-），男，本科，主管技师，从事实验室管理相关工作。
李斌（1972-），男，硕士，副主任技师，从事实验室管理相关工作。并列第一作者。
通讯作者：曾宪飞，男，博士，主任技师，医师，E-mail：mumufly@126.com。
更新日期/Last Update: 2023-07-15