[1]汪 敏a,余 帅b,胡国凤.乳腺癌组织中APOA4和LRG1水平表达与临床病理特征的相关性研究[J].现代检验医学杂志,2025,40(01):127-131.[doi:10.3969/j.issn.1671-7414.2025.01.024]
　WANG Mina,YU Shuaib,HU Guofeng.Correlation between the Level Expression of APOA4 and LRG1 in Breast Cancer Tissues and Clinicopathological Features[J].Journal of Modern Laboratory Medicine,2025,40(01):127-131.[doi:10.3969/j.issn.1671-7414.2025.01.024]
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乳腺癌组织中APOA4和LRG1水平表达与临床病理特征的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年01期
页码:
127-131
栏目:
论著
出版日期:
2025-01-15

文章信息/Info

Title:
Correlation between the Level Expression of APOA4 and LRG1 in Breast Cancer Tissues and Clinicopathological Features
文章编号:
1671-7414（2025）01-127-05
作者:
汪 敏1a; 余 帅1b; 胡国凤2
（1. 富顺县妇幼保健院a. 外科；b. 妇科，四川富顺 643200；2. 自贡市第三人民医院普外科，四川自贡 643020）
Author(s):
WANG Min1a; YU Shuai1b; HU Guofeng2
（1a. Deparment of Surgery; 1b Department of Gynecology，Fushun County Maternal and Child Health Hospital，Sichuan Fushun 643200，China；2. Department of General Surgery， the Third People’s Hospital of Zigong City，Sichuan Zigong 643020，China）
关键词:
乳腺癌; 载脂蛋白A Ⅳ; 富亮氨酸α-2 糖蛋白1
分类号:
R737.9；R730.43
DOI:
10.3969/j.issn.1671-7414.2025.01.024
文献标志码:
A
摘要:
目的 研究乳腺癌组织中载脂蛋白A Ⅳ（APOA4）和富亮氨酸α-2 糖蛋白1（LRG1）蛋白表达与临床病理特征的相关性。方法 收集120 例于2020 年3 月～ 2022 年9 月在自贡市第三人民医院行手术治疗并经术后病理确诊为乳腺癌的患者临床资料。应用蛋白质免疫印迹（WB）法检测乳腺癌组织及相应癌旁组织（距癌组织边缘>5cm）中APOA4 与LRG1 蛋白表达情况。分析APOA4 及LRG1 蛋白表达与乳腺癌临床病理特征的关系；Pearson 相关分析APOA4 与LRG1 蛋白表达的相关性；Kaplan-Meier 生存法分析APOA4 和LRG1 蛋白表达与预后的关系。结果 WB 检测结果显示，乳腺癌组织中APOA4（2.81±0.74）与LRG1（1.01±0.26）蛋白表达水平均显著高于乳腺正常组织（1.26±0.38，0.57±0.12），差异具有统计学意义（t=20.411，16.832，均P<0.05）；且Pearson 相关分析显示，在乳腺癌组织中APOA4 与LRG1 蛋白表达呈正相关（r=0.624，P=0.014）。cTNM 分期II ～ III 期、ER 阳性、PR 阳性、并发淋巴结转移的乳腺癌组织中APOA4 和LRG1 蛋白的表达水平高于cTNM 分期Ⅰ期、ER 阴性、PR 阴性、无淋巴结转移的乳腺癌组织，差异具有统计学意义（t=1.770 ～ 3.284，均P<0.05），且BMI ≥ 23.5kg/m2 的乳腺癌组织中APOA4 蛋白表达高于BMI ＜ 23.5kg/m2 的乳腺癌组织，差异具有统计学意义（t=1.736，P=0.043）。Kaplan-Meier 生存分析显示，APOA4高表达组和LRG1 高表达组的累积总生存率（83.13%，84.93%）均低于APOA4 低表达组和LRG1 低表达组（94.74%，91.49%），差异具有统计学意义（Log Rank χ2=4.778，3.956，P =0.029，0.047）。结论 APOA4 和LRG1 蛋白在乳腺癌组织中均呈高表达，且共同参与了乳腺癌的发生及侵袭，有望成为诊疗及预后预测的参考指标。
Abstract:
Objective To study the correlation between apolipoprotein AⅣ (APOA4) and leucine-rich α-2 glycoprotein 1 (LRG1) expression and clinicopathological features in breast cancer tissues. Methods Clinical data of 120 patients diagnosed with breast cancer after surgical treatment and postoperative pathology were collected from the Third People’s Hospital of Zigong City from March 2020 to September 2022. Western blotting (WB) was used to observe the expression of APOA4 and LRG1 in breast cancer tissues and corresponding paracuncinoma tissues (>5cm from the margin of cancer tissues). Analyzed the relationship between the expression of APOA4 and LRG1 and the clinicopathological features of breast cancer. The correlation between the expression of APOA4 and LRG1 was analyzed by Pearson methed. Kaplan-Meier survival analysis of the relationship between APOA4 and LRG1 protein expression and prognosis. Results The results of WB detection showed that the expression levels of APOA4 （2.81±0.74）and LRG1（1.01±0.26）in breast cancer tissues were significantly higher than those in normal breast tissues （1.26±0.38，0.57±0.12），and the differences were statistically significant (t=20.411，16.832，all P<0.05). Pearson correlation analysis showed that APOA4 was positively correlated with LRG1 protein expression in breast cancer tissues (r=0.624, P=0.014). The expression levels of APOA4 and LRG1 proteins in breast cancer tissues with ER positive, PR positive and lymph node metastasis in cTNM stage II to III were higher than those in breast cancer tissues with ER negative, PR negative and no lymph node metastasis in cTNM stage I , and the differences were statistically significant (t=1.770 ～ 3.284，all P<0.05). The expression of APOA4 protein in breast cancer tissues with BMI ≥ 23.5kg/m2 was higher than that in breast cancer tissues with BMI<23.5kg/m2, and the difference was statistically significant (t=1.736, P=0.043). Kaplan-Meier survival analysis showed that the cumulative overall survival rates (83.13%，84.93%) of the APOA4 high expression group and LRG1 high expression group were lower than those of the APOA4 low expression group and LRG1 low expression group (94.74%， 91.49%, and the differences were statistically significant (Log Rankχ2==4.778，3.956，P=0.029，0.047). Conclusion Both APOA4 and LRG1 proteins are highly expressed in breast cancer tissues, and are involved in the occurrence and invasion of breast cancer. They are expected to become a reference index for diagnosis and treatment and prognosis prediction.

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备注/Memo

备注/Memo:
基金项目：2020 年四川医学会科研课题（S20071）。
作者简介：汪敏（1980-），女，本科，副主任医师，研究方向：甲状腺癌、乳腺癌诊治，E-mail：RBMKK6s@126.com。
更新日期/Last Update: 2025-01-15