[1]杨立芬,何建清,尹立新,等.人卵巢癌细胞SKOV3中壳多糖酶3样蛋白1的表达和对细胞增殖及侵袭的影响实验研究[J].现代检验医学杂志,2021,36(04):31-34.[doi:10.3969/j.issn.1671-7414.2021.04.007]
 YANG Li-fen,HE Jian-qing,YIN Li-xin,et al.Expression of Chitinase 3-like Protein 1 in Human Ovarian Cancer Cell Skov3and Its Effect on Cell Proliferation and Invasion[J].Journal of Modern Laboratory Medicine,2021,36(04):31-34.[doi:10.3969/j.issn.1671-7414.2021.04.007]
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人卵巢癌细胞SKOV3中壳多糖酶3样蛋白1的表达和对细胞增殖及侵袭的影响实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年04期
页码:
31-34
栏目:
论 著
出版日期:
2021-07-31

文章信息/Info

Title:
Expression of Chitinase 3-like Protein 1 in Human Ovarian Cancer Cell Skov3and Its Effect on Cell Proliferation and Invasion
文章编号:
1671-7414(2021)04-031-04
作者:
杨立芬1何建清1尹立新2武 军1高 然1
(1. 唐山市妇幼保健院妇产科 河北唐山 063000;2. 唐山市南湖医院急诊科,河北唐山 063000)
Author(s):
YANG Li-fen1 HE Jian-qing1 YIN Li-xin2 WU Jun1 GAO Ran1
(1. Department of Obstetrics Gynecology, Tangshan Maternal and Child Health Hospital, Hebei Tangshan 063000, China;2. Department of Emergency Medicine, Nanhu Hospital of Tangshan City, Hebei Tangshan 063000, China)
关键词:
壳多糖酶3 样蛋白1卵巢癌增殖侵袭能力
分类号:
R737.31;R730.43
DOI:
10.3969/j.issn.1671-7414.2021.04.007
文献标志码:
A
摘要:
目的 探讨壳多糖酶3 样蛋白1(chitinase-3-like-protein-1, CHI3L1)在卵巢癌细胞SKOV3 中的表达和对SKOV3 细胞增殖和侵袭的影响。方法 构建pll3.7-CHI3L1 shRNA 重组质粒,转染卵巢癌细胞SKOV3,使得卵巢癌细胞中的CHI3L1 基因沉默;pll3.7-CHI3L1 shRNA 重组质粒转染卵巢癌细胞SKOV3,同时转染空载质粒作为阴性对照,通过Western Blot 检测SKOV3 细胞中CHI3L1 蛋白表达水平;平板细胞克隆形成试验观察对卵巢癌细胞SKOV3 增殖的影响;采用Transwell 实验检测卵巢癌细胞SKOV3 侵袭能力的变化。结果 成功构建pll3.7-CHI3L1 shRNA 重组质粒,转染卵巢癌细胞SKOV3 后,观察到大量的绿色荧光细胞,且和对照组相比,pll3.7-CHI3L1 shRNA 转染组卵巢癌细胞SKOV3 中CHI3L1 蛋白表达水平下降,卵巢癌SKOV3 细胞的克隆数目减少,侵袭能力下降。结论 卵巢癌细胞SKOV3 中CHI3L1 的表达下调,抑制了SKOV3 细胞的增殖和侵袭能力。

参考文献/References:

[1] TAYLOR S E, WIELD A, FANG Yusi, et al. Endocrinebiomarkers in low-grade serous ovarian cancers(LGSC)and serous ovarian tumors of low malignantpotential(LMP)[J]. Journal of Clinical Oncology, 2020,38(suppl 15): e18045.
[2] SHI Yuchuan, HE Runhua, YANG Yu, et al. CircularRNAs: novel biomarkers for cervical, ovarian andendometrial cancer (review)[J]. Oncology Reports,2020, 44(5): 1787-1798.
[3] 黄梨, 龙佑梅, 付义霞, 等. 紫檀芪对卵巢癌SKOV3细胞凋亡和糖酵解的影响及机制研究[J]. 实用肿瘤学杂志,2019,33(6):502-507.HUANG Li, LONG Youmei, FU Yixia, et al. Effect ofpterostilbene on apoptosis and glycolysis of ovariancancer SKOV3 cells and its mechanism [J]. PracticalOncology Journal, 2019, 33(6): 502-507.
[4] CHIANG Y C, LIN H W, CHANG C F, et al.Overexpression of CHI3L1 is associated withchemoresistance and poor outcome of epithelial ovariancarcinoma.[J]. Oncotarget, 2015, 6(37) : 39740-39755.
[5] SU C W, CHEN M K, HUNG W C, et al. Functionalvariant of CHI3L1 gene is associated with neck metastasisin oral cancer[J]. Clinical Oral Investigations,2019, 23(6) : 2685-2694.
[6] WANG Y, ZHONG M, WANG W, et al. CHI3L1regulates APAP-induced liver injury by promotingmacrophage infiltration[J]. European review formedical and pharmacological sciences, 2019, 23(11) :4996-5003.
[7] LUO Dingyuan, CHEN Haibo, LUO Penghui ,et al.CHI3L1 overexpression is associated with metastasisand is an indicator of poor prognosis in papillarythyroid carcinoma[J]. Cancer Biomarkers: Section A ofDisease Markers, 2017, 18(3) : 273-284.
[8] ANSARI K I, BHAN A, LIU Xueli, et al. AstrocyticIGFBP2 and CHI3L1 in cerebrospinal fluid drivecortical metastasis of HER2+breast cancer[J]. Clinical& Experimental Metastasis, 2020, 37(3): 401-412.
[9] LIU Kaitai, JIN Ming, YE Shuang, et al. CHI3L1promotes proliferation and improves sensitivity tocetuximab in colon cancer cells by down‐regulatingp53[J]. Journal of Clinical Laboratory Analysis, 2020,34(1) : e23026.
[10] KAINULAINEN H. CHI3L1--a novel myokine[J].Acta Physiologica (Oxford, England), 2016, 216(3):260-261.
[11] 张巧娣, 谢而付, 凌芸, 等. 壳多糖酶3 样蛋白1 与甲胎蛋白在肝细胞癌诊断中的比较[J]. 现代检验医学杂志,2017,32(1):45-47, 52.ZHANG Qiaodi, XIE Erfu, LING Yun, et al.Comparative diagnostic value of chitinase 3-like 1protein and AFP in the diagnosis of hepatocellularcarcinoma [J]. Journal of Modern Laboratory Medicine,2017, 32(1):45-47,52.
[12] 石玉如,岳莉,赵长城,等.血清壳多糖酶3 样蛋白1 检测在不同肝脏疾病中的应用价值[J]. 现代检验医学杂志,2017,32(6):39-42.SHI Yuru, YUE Li, ZHAO Changcheng, et al. Clinicalapplication value of chitinase-3-like protein 1 inpatients with different liver diseases [J]. Journal ofModern Laboratory Medicine, 2017, 32(6):39-42.
[13] LIBREROS S, GARCIA-AREAS R, SHIBATA Y, et al.Induction of proinflammatory mediators by CHI3L1 isreduced by chitin treatment: decreased tumor metastasisin a breast cancer model[J]. International Journal ofCancer, 2012, 131(2): 377-386.
[14] KU B M, LEE Y K, RYU J, et al. CHI3L1 (YKL-40) isexpressed in human gliomas and regulates the invasion,growth and survival of glioma cells[J]. InternationalJournal of Cancer, 2011, 128(6): 1316-1326.
[15] KAWADA M, SENO H, KANDA K, et al. Chitinase3-like 1 promotes macrophage recruitment andangiogenesis in colorectal cancer[J]. Oncogene, 2012,31(26): 3111-3123.
[16] KIM E G, KIM M N, HONG JY, et al. Chitinase 3-Like1 contributes to food allergy via M2 macrophagepolarization[J]. Allergy Asthma & ImmunologyResearch, 2020, 12(6): 1012-1028.
[17] WURM J, BEHRINGER S P, RAVI V M, et al.Astrogliosis releases Pro-Oncogenic chitinase 3-Like 1causing MAPK signaling in glioblastoma[J]. Cancers,2019, 11(10): 1437.

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备注/Memo

备注/Memo:
基金课题:2019 年度河北省医学科学研究课题(编号20191534)。
作者简介: 杨立芬(1981-),女,硕士,主治医师,研究方向:妇科肿瘤及内分泌,E-mail:yking7342@163.com。
更新日期/Last Update: 1900-01-01