[1]汪怀周,陈 燕,岳展伊,等.C反应蛋白测定两种检测系统间结果的比较[J].现代检验医学杂志,2016,31(03):127-130.[doi:10.3969/j.issn.1671-7414.2016.03.036]
　WANG Huai-zhou,CHEN Yan,YUE Zhan-yi,et al.Comparison of Different Assay System for C-reactive Protein Detection[J].Journal of Modern Laboratory Medicine,2016,31(03):127-130.[doi:10.3969/j.issn.1671-7414.2016.03.036]
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C反应蛋白测定两种检测系统间结果的比较()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
127-130
栏目:
研究简报·实验技术
出版日期:
2016-06-15

文章信息/Info

Title:
Comparison of Different Assay System for C-reactive Protein Detection
作者:
汪怀周; 陈 燕; 岳展伊; 朱荣荣; 邓安梅
第二军医大学长海医院实验诊断科,上海 200433
Author(s):
WANG Huai-zhou; CHEN Yan; YUE Zhan-yi; ZHU Rong-rong; DENG An-mei
Department of Laboratory Diagnosis, Changhai Hospital of Second Military Medical University,Shanghai 200433,China
关键词:
床旁即时检测;  C反应蛋白;  红细胞压积;  评价研究
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2016.03.036
文献标志码:
A
摘要:
目的 对两种不同检测系统测定C反应蛋白(C-reactive protein,CRP)的结果进行比对和偏倚分析,以评价不同检测系统CRP测定结果的可比性。方法 参照美国临床实验室标准研究所(CLSI)EP9-A2文件要求,收集长海医院急诊患者乙二胺四乙酸二钾(EDTA-K2)抗凝血50例,同时使用全自动免疫分析仪(Beckman Immage 800)和免疫荧光分析仪(i-CHROMATM Reader)检测待测样本中的CRP浓度,评价两种方法检测结果的相关性。结果 使用血浆标本时,两方法相关性良好,回归方程为Y=1.076 5X-3.031 5,R2=0.986。全血测定的回归方程为Y=0.882 6X-1.180 8,R2=0.931 1。对于红细胞压积(HCT)较低的标本(<30.45%)使用纠正公式:全血CRP(纠正后)=全血CRP结果(纠正前)/(1-HCT)。Y(纠正后)=1.006 8X-3.612 2,R2=0.950 9。结论 两种检测系统血浆CRP的测定结果具有可比性,适合临床应用。遇HCT较低的标本时,通过校正公式校正,可提高全血结果的可比性。
Abstract:
Objective To evaluate comparability of two different assay system for detecting CRP.Methods Following the profile of Clinical and Laboratory Standard Institute(CLSI)document EP9-A2, 50 blood samples with anti-coagulant EDTA-2K were collected from emergencypatients at Changhai Hospital.The test result of samples by the i-CHROMA Reader was compared and evaluated with those by Beckman Immage 800.Results The linear regression equation for plasma CRP was:Y=1.076 5X-3.031 5,R2=0.986.The linear regression equation for whole blood CRP was:Y=0.882 6X-1.180 8,R2=0.931 1.For whole blood samples with low HCT(<30.45%).Used correction equation:CRP(after corrected)=CRP(before corrected)/(1-HCT).The regression equation(after corrected)was:Y=1.006 8X-3.612 2,R2=0.950 9.Conclusion CRP concentration detected by i-CHROMA showed good correlation and comparability comparedto laboratory reference system by using plasma samples.Results form whole bloodsamples with low HCT should be corrected to improve comparability.

参考文献/References:

[1] Sankar V,Webster NR.Clinical application of sepsis biomarkers[J].Journal of Anesthesia,2013,27(2):269-283.
[2] 张 婷,丁 爽,李洪春,等.血清PCT,CRP及NE U%联合检测对细菌性血流感染的早期诊断价值[J].现代检验医学杂志,2014,29(3):75-77. Zhang T,Ding S,Li HC,et al.Early diagnostic value of combined detection of serum PCT,CRP and NEU% in patients with bacterial bloodstream infection[J].J Mod Lab Med,2014,29(3):75-77.
[3] 卓兰云,黎小琼,何 敏,等.POCT仪器与全自动生化分析仪C反应蛋白检测结果的比对[J].广东医学,2014,35(1):110-111. Zhuo LY,Li XQ,He M,et al.[J].Guangdong Medical Journal,2014,35(1):110-111.
[4] Lelubre C,Anselin S,Boudjeltia ZK,et al.Interpretation of C-reactiveprotein concentrations in critically ill patients[J].Bio Med Research International,2013,2013(7):124021.
[5] Chandra P,Suman P,Airon H,et al.Prospects and advancements in C-reactive protein detection[J].World Journal of Methodology,2014,4(1):1-5.
[6] Oh SW,Moon JD,Park SY,et al.Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing[J].Clin Chim Acta,2005,356(1/2):172-177.
[7] Ciftci IH, Koroglu M, Karakece E.Comparison of novel and familiar commercial kits for detection of C-reactive protein levels[J].World Journal of Microbiology and Biotechnology,2014,30(8):2295-2298.
[8] 邓芳梅,林晓文,李德发.i-CHROMA TMREADER免疫荧光分析仪性能评价[J].临床输血与检验,2010,12(2):107-109. Deng FM,Lin XW,LI DF.Preformance evaluation of the i-CHROMATMREADER fluorescence immunoassay analyzer[J].J Clin Transfus Lab Med,2010,12(2):107-109.
[9] 金明超,任春云,王 星.i-CHROMATMReader免疫荧光分析仪测定超敏C反应蛋白性能评价[J].实验与检验医学,2014,32(2):227-229. Jin MC,Ren CY,Wamg X.[J].Experimental and Laboratory Medicine,2014,32(2):227-229.
[10] Ahn JS,Choi S,Jang SH,et al.Development of a point-of-care assay system for high-sensitivity C-reactive protein in whole blood[J].Clin Chim Acta,2003,332(1):51-59.
[11] 隆维东,李 坚,刘万彬.不同红细胞压积对全血CRP测定的影响及校正措施[J].国际检验医学杂志,2012,33(1):107-109. Long WD,Li J,Liu WB.International Journal of Laboratory Medicine,2012,33(1):107-109.
[12] 包安裕,李 艳.床旁即时检测与检验科常规检测C反应蛋白的比较[J].现代检验医学杂志,2011,26(3):99-101. Bao AY,Li Y.Comparison of C-reactive protein detection using point-of-care testing and routine analysis system in clinical laboratory[J].J Mod Lab Med,2011,26(3):99-101.
[13] 苏 霞.1 400例门诊患者末梢血与静脉血血常规和高敏C-反应蛋白检测方法及检测仪器的评价[J].国际老年医学杂志,2012,33(6):246-248. Su X.Evaluation of the detection method and detector of peripheral bloodand venous blood WBC and hs-CRP[J].Intermational Journal of Geriatrics,2012,33(6):246-248.
[14] 潘惠芬,顾文红,赵 缜,等.急诊CRP检测方法的选择与临床应用[J].国际检验医学杂志,2012,33(7):849-851. Pan HF,Gu WH,Zhao Z,et al.International Journal of Laboratory Medicine,2012,33(7):849-851.
[15] 叶昌远,程晓玲.全血快速C反应蛋白检测的应用[J].中国社区医师:医学专业,2012,14(9):272.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81471605,8273282,81202353),上海市申康基金(CHDC22014014),长海医院基金(CH125530300)。 作者简介:汪怀周(1978-),男,硕士,主管技师,研究方向:临床检验,E-mail:whz_sh@163.com。 作者简介:陈 燕(1977-),共同第一作者,女,主管技师,E-mail:chenyan770628@163.com。
更新日期/Last Update: 2016-06-25