[1]钱忠萍,凌 晨,祁松楠,等.围非小细胞肺癌手术期T细胞含量变化的研究[J].现代检验医学杂志,2016,31(05):55-58.[doi:10.3969/j.issn.1671-7414.2016.05.014]
　QIAN Zhong-ping,LING Chen,QI Song-nan,et al.Content Variation of T Cells in Perioperative Patients with Non-Small Cell Lung Cancer[J].Journal of Modern Laboratory Medicine,2016,31(05):55-58.[doi:10.3969/j.issn.1671-7414.2016.05.014]
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围非小细胞肺癌手术期T细胞含量变化的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
55-58
栏目:
论著
出版日期:
2016-10-21

文章信息/Info

Title:
Content Variation of T Cells in Perioperative Patients with Non-Small Cell Lung Cancer
文章编号:
1671-7414(2016)05-055-04
作者:
钱忠萍1; 凌 晨1; 祁松楠1; 韩清珍1; 邱 骏1; 顾宗江2; 顾国浩1
1.江苏省苏州大学附属第一医院检验科,江苏苏州 215000;
2.苏州大学医学部免疫学系,江苏苏州 215000
Author(s):
QIAN Zhong-ping1; LING Chen1; QI Song-nan1; HANQing-zhen1; QIU Jun1; GU Zong-jiang2;  GU Guo-hao1
1.Department of Clinical Laboratory,the FirstAffiliated Hospital of Soochow University in Jiangsu Province,Jiangsu Suzhou 215000,China;
2.Department of Immunology, Department of Medicine,Soochow University in Jiangsu Province,Jiangsu Suzhou 215000,Ch
关键词:
非小细胞肺癌;  T淋巴细胞亚群;  Th1;  Th2;  免疫系统紊乱
分类号:
R734.2; R730.43
DOI:
10.3969/j.issn.1671-7414.2016.05.014
文献标志码:
A
摘要:
目的 通过分析非小细胞肺癌(non-small-cell carcinoma,NSCLC)患者外周血淋巴细胞Th1和Th2细胞水平,研究NSCLC患者术前术后T淋巴细胞亚群的变化,为临床上对NSCLC疾病的预防、诊断及治疗提供参考依据。方法 收集NSCLC患者(60名),健康体检者(60名)全血(EDTA抗凝处理),采用流式细胞术检测外周血淋巴细胞CD3+,CD4+,CD8+T细胞百分比以及Th1和Th2细胞水平,并且用血细胞分析仪检测其淋巴细胞绝对值。结果 NSCLC术前和正常对照组CD3+,CD4+,CD8+T细胞百分比(%)分别为58.40±10.27 vs 66.58±6.84,31.32±8.65 vs 39.40±6.43,34.23±8.00 vs 24.31±8.16,CD4+/CD8+比值为0.96±0.23 vs 1.58±0.23,差异均具有统计学意义(t=-6.726～14.916,P均<0.05)。NSCLC术后1～3天组和正常对照组CD3+,CD4+,CD8+T细胞百分比(%)分别为56.31±8.00 vs 66.58±6.84,27.72±7.55 vs 39.40±6.43,33.69±7.10 vs 24.31±8.16,CD4+/CD8+比值为0.87±0.31vs 1.58±0.23,差异均具有统计学意义(t=-6.720～14.367,P均<0.05)。与正常对照组相比,NSCLC术后4～7天组CD4+T细胞百分比(%)为33.23±4.13 vs 39.40±6.43,差异具有统计学意义(t=6.257,P<0.05)。在辅助性T细胞亚群中,NSCLC术前与正常对照组Th1,Th2细胞含量(×10/μl)分别为6.79±1.34 vs 12.52±3.56,4.82±0.51 vs 2.32±0.82,Th1/Th2比值为1.39±0.84 vs 5.36±1.42,差异均具有统计学意义(t=-20.087～18.630,P均<0.05)。NSCLC术后1～3天组与正常对照组Th1细胞含量(×10/μl)为8.86±1.52 vs 12.52±3.56,术后4～7天组与正常对照组Th1细胞含量(×10/μl)为7.02±1.27 vs 12.52±3.56,差异均具有统计学意义(t=7.339～11.275,P均<0.05)。结论 NSCLC患者免疫功能紊乱,表现在T淋巴细胞数和辅助性T细胞的数量减少,T细胞亚群失调,明显趋向Th2细胞; 另外可能在癌细胞的不断刺激下,细胞免疫功能亢进杀伤性T细胞增多,经过手术治疗后杀伤性T细胞开始降低。
Abstract:
Objective To research the mechanism of the changes of T lymphocyte subtypes and provide reference for clinically prevention,diagnosis and treatment for NSCLC through analysis of the expression of Th1,Th2 in Non-small-cell carcinoma(NSCLC)patients.Methods Whole blood(EDTA anticoagulant treatment)from 60 NSCLC patients and 60 healthy subjects were collected to detect of the expression of CD3+T cells,CD4+Tcells and CD8+T cells on T lymphocytes and the levels of Th1 and Th2 cellsby flow cytometer(FCM),and the absolute value of T lymphocyte by hematology analyzer.Results Compared with normal control group, after surgery 1～3 days NSCLC groups,the percent of CD3+T,CD4+T,CD8+Tcells and the CD4+/CD8+ ratio in the NSCLC patients before surgery weresignificantly reduced 58.40±10.27 vs 66.58±6.84,31.32±8.65 vs 39.40±6.43,34.23±8.00 vs 24.31±8.16,0.96±0.23 vs 1.58±0.23(t=-6.726～14.916,P<0.05).The percent of CD3+T,CD4+T,CD8+T cells and the CD4+/CD8+ ratio in the NSCLC patients after surgery 1～3 days werealso significantly decreased 56.31±8.00 vs 66.58±6.84,27.72±7.55 vs 39.40±6.43,33.69±7.10 vs 24.31±8.16,0.87±0.31 vs 1.58±0.23(t=-6.720～14.367,P<0.05).The percent of CD4+T cells in the NSCLC patients after surgery 4～7 days was increased 33.23±4.13 vs 39.40±6.43(t=6.257,P<0.05).Compared with the control group,within the helper T cell subsets,the cell content of Th1,Th2 cells(×10/μl)and the Th1/Th2 ratio weresignificantly changed in different extent in the NSCLC group before surgery 6.79±1.34 vs 12.52±3.56,4.82±0.51 vs 2.32±0.82,1.39±0.84 vs 5.36±1.42(t=-20.087～18.630,P<0.05).The content of Th1 cells was lower inthe NSCLC patients after 1～3 days and 4～7 days 8.86±1.52 vs 12.52±3.56,7.02±1.27 vs 12.52±3.56(t=7.339～11.275,P<0.05).Conclusion The NSCLC patients presented immune dysfunction,like T lymphocytes and helper T cells decreased and Th2 cells were clearly in the ascendant.Also,the cytotoxic T cells increased by the stimulation of cancer cells,but they began to decrease after the surgery.

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备注/Memo

备注/Memo:

作者简介:钱忠萍(1979-),女,硕士,主管技师,主要从事临床检验工作,研究方向为肿瘤免疫,Tel:13372144231,E-mail:moony_u@163.com。
通讯作者:顾国浩,男,硕士生导师,教授,主要研究领域为分子免疫学和分子生物学检验,Tel:13962524418,E-mail:13962524418@163.com。
更新日期/Last Update: 2016-10-30