[1]唐努尔·艾尔肯,阿孜古·祖农.盐酸罗哌卡因对人乳腺癌细胞运动作用的研究[J].现代检验医学杂志,2017,32(03):128-130.[doi:10.3969/j.issn.1671-7414.2017.03.035]
　TANGNUER·Aierken,AZIGU·Zunong.Study on Effect of Ropivacaine on Migration of Breast Cancer Cell s[J].Journal of Modern Laboratory Medicine,2017,32(03):128-130.[doi:10.3969/j.issn.1671-7414.2017.03.035]
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盐酸罗哌卡因对人乳腺癌细胞运动作用的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
128-130
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Study on Effect of Ropivacaine on Migration of Breast Cancer Cell s
文章编号:
1671-7 414(2017)03-128-03
作者:
唐努尔·艾尔肯1; 阿孜古·祖农2
1.新疆喀 什地区第一人民医院麻醉科，新疆喀什 844000；
2.新疆喀什地区肺科医院检验科，新疆喀什 844000
Author(s):
TANGNUER·Aierken1; AZIGU·Zunong2
1.De partment of Anesthesia， the First People’s Hospital of Xinjiang Kashi，Xinjiang Kashi 844000，Chi na；
2.Department of Clinical Laboratory，the Pulmonary Hospital of Xinjiang Kashi，Xinjiang Kashi 844000，China
关键词:
盐酸罗哌卡因; 乳腺癌; 迁移; 趋化运动
分类号:
R737.9；R730.43
DOI:
10.3969/j.issn.1671-7414.2017.03.035
文献标志码:
A
摘要:
目的 探讨不同剂量的盐酸罗哌卡因对乳腺癌MDA-MB-231 细胞生长和运动行为学的影响。方法 乳腺癌MDA-MB-231细胞常规 培养后加入不同剂量盐酸罗哌卡因。分组为低剂量组150 μg/ml(A组)，中剂量组250 μg/m l(B组)和高剂量组450 μg/ml(C组)，设空白对照组(D组)。MTT法检测细胞生长情况，同时 观察细胞划痕和趋化运动能力变化。结果 低剂量盐酸罗哌卡因组乳 腺癌细胞生长不受影响，而中剂量和高剂量组乳腺癌细胞生长受到较大的抑制，与对照组相 比差异有统计学意义(t=8.845～11.654，P<0.05)。低剂量盐酸罗哌卡因组乳腺癌细 胞的迁移和趋化运动能力与对照组相比差异无统计学意义(t=1.025～1.124，P>0.05 )。中剂量组和高剂量组乳腺癌细胞的迁移和趋化运动能力比对照组明显减低，组间差异有 统计学意义(t=6.054～12.412，t=8.654～10.214，均P<0.05)。结论 盐酸罗哌卡因能抑制乳腺癌细胞生长和运动，具有剂量依赖性。
Abstract:
Objective To observe the different behavior of migration in breast cancer cells，when exposure to ropivacaine of different conc entrations．Methods The breast cancer cells MDA-MB-231 were inoculated into culture medium．They were divided into four groups：low-do se group (150 μg/ml，A group)，medium-dose group (250 μg/ml，B group)，high- dose group (450 μg/ml，C group) and control group (D group)，and detected the c ell proliferation，abilities of cell scratch and chemotaxis．Results MDA-MB-231 cell viability of B and C group was significantly low er than that of D group (t=8.845～11.654，P<0.05)．Migration and chemotax is abilities of B group and C group were decreased than those of D group (t=6 .054～12.412，t=8.654～10.214，P<0.05)，and there was no difference betwe en A group and D group (t=1.025～1.124，P>0.05)．Conclusion Ropivacaine could restrain the viability and migration abilities of b reast cancer cells depending on its different concentrations．

参考文献/References:

［1］ Browaeys-Poly E，Perdereau D，Lescuyer A，et al．Akt interaction with PLC(gamma) regulates the G(2)/M transition triggered by FGF receptors from MDA-MB-231 breast cancer cells［J］．Anticancer Research，2009，29(12)：4965 -4969．
［2］ 夏 明，王少华，童建华，等．不同浓度罗哌卡因对乳腺癌MCF-7细胞增殖和细胞 周期的作用研究［J］．临床麻醉学杂志，2016，32(7)：680-683． Xia M，Wang SH，Tong JH，et al．The effect of ropivacaine on prolife ration and cell cycle of human breast cancer MCF-7 cells［J］．J Clin Anesthesi ol，2016，32(7)：680-683．
［3］ Sala G，Dituri F，Raimondi C，et al．Phospholipase C gamma 1 is required for metastasis development and progression［J］．Cancer Res，2008，68(24)：10187 -18196．

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备注/Memo

备注/Memo:

作者简介：唐努尔·艾尔肯(1975-)，女，副主任医师，主要从事临床麻醉工作。
更新日期/Last Update: 2017-06-25