[1]孟凡凡,齐晓林,黄奕江,等.乌司他丁干预对水下爆炸致兔急性肺损伤TNF-α表达的影响[J].现代检验医学杂志,2017,32(03):131-132,136.[doi:10.3969/j.issn.1671-7414.2017.03.036]
 MENG Fan-fan,QI Xiao-lin,HUANG Yi-jiang,et al.Effect of Ulinastatin on the Expression of TNF-α in the Rabbits with Acute Lung Injury Induced by Underwater Explosion[J].Journal of Modern Laboratory Medicine,2017,32(03):131-132,136.[doi:10.3969/j.issn.1671-7414.2017.03.036]
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乌司他丁干预对水下爆炸致兔急性肺损伤TNF-α表达的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
131-132,136
栏目:
研究简报·实验技术
出版日期:
2017-05-25

文章信息/Info

Title:
Effect of Ulinastatin on the Expression of TNF-α in the Rabbits with Acute Lung Injury Induced by Underwater Explosion
文章编号:
1671-7414(2017)03 -131-03
作者:
孟凡凡1齐晓林1黄奕江1叶长青1王前进1 马宏昊2邵先安1
1.解放军第123医院检验科, 安徽蚌埠 233015;
2.中国科技大学近代力学系爆炸实验室,合肥 230027
Author(s):
MENG Fan-fan1QI Xiao-lin1HUANG Yi-jiang1 YE Chang-qing1WANG Qian-jin1MA Hong-hao2 SHAO Xian-an1
1.Department of Clinical Laboratory,the 123 th Hospital of PLA, Anhui Bengbu,233015,China;
2.the Explosion Laboratory of Modern Mechani cs, in Science and Technology University of China,Hefei 230027,China
关键词:
水下爆炸急性肺损伤乌司他丁肿瘤坏死因子-α
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2017.03.036
文献标志码:
A
摘要:
目的 探讨乌司他丁干预对水下爆炸致兔急性肺损伤时肿瘤 坏死因子(tumor necrosis factor-alpha,TNF-α)的影响。方法 〖HT5”K 〗健康新西兰兔随机分为爆炸损伤组与乌司他丁治疗组,以水下爆炸致兔急性肺损伤,用免 疫酶联法检测爆炸后4,12和24 h血清中TNF-α浓度。以SPSS17.0统计软件进行分析,P <0.05为差异具有统计学意义。结果 爆炸后4 h乌司他丁组TNF- α浓度为386.90±109.22 ng/L,爆炸损伤组TNF-α浓度为538.20±201.43 ng/L,二 者相比无变化(t=2.088,P=0.051);爆炸后12 h乌司他丁组TNF-α浓度为400.60± 78.98 ng/L,爆炸损伤组TNF-α浓度为573.80±178.24 ng/L,二者相比差异有统计 学意义(t=2.809,P=0.012);24 h乌司他丁组TNF-α浓度为356.10±130.99 ng/ L,爆炸损伤组为552.30±169.64 ng/L,二者相比差异也有统计学意义(t=2.895,P =0.010)。结论 乌司他丁干预下实验兔在爆炸12 h后血清TNF- α降低。
Abstract:
Objective The expression of TNF-α was detecte d in sera of rabbits treated by ulinastatin with acute lung injury induced by un derwater explosion.Methods Rabbits were randomly divided into two groups such as the injured group and ulinastatin therapy group.Estab lished underwater explosion device was used to cause acute lung injury in rabbit s. TNF-α in sera of the rabbits were measured by ELISA at 4,12 and 24 hours after the explosion.The SPSS17.0 software was used to analyze the data and P <0.05 was considered to be significant.Results There was no significant difference between the concentrations of TNF-α in the sera of rabbits in the injure group (538.20±201.43 ng/L) and that of in the ulinas tatin group (386.90±109.22 ng/L,t=2.088,P=0.051) at 4 hours after bur st.However,there was evidently decreased in the level of TNF-α in the ulinas tatin group (400.60±78.98 ng/L) compared with the injury group (573.80±178 .24 ng/L,t=2.809,P=0.012) at 12 hours after burst.Moreover and TNF- α in the ulinastatin group (356.10±130.99 ng/L) was also decreased compared to the injure group (552.30±169.64 ng/L,t=2.895,P=0.010) at 24 hour s after burst.Conclusion The TNF-α expression in sera of the rabbits in ulinastatin group were dramatically decreased than that of in injury group at 12 hours after burst,which may be benefit to rabbits with acute lung injury induced by underwater explosion.

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备注/Memo

备注/Memo:
基金项目:南京军区医学重点课题资助项目(14ZD20)。
作者简介:孟凡凡(1985-),女,本科,主管技师,主要从事临床 输血/分子免疫工作。
通讯作者:齐晓林(1971-),男,硕士,教授,硕士生导师,研究 方向:临床输血/医院管理。
更新日期/Last Update: 2017-06-25